Methionine supply during the peripartum period and early lactation alter immunometabolic gene expression in cytological smear and endometrial tissue of holstein cows

Guadagnin, A.R.; Velasco-Acosta, D.A.; Stella, Stephanie; Luchini, D.; Cardoso, F.C.

doi:10.1016/j.theriogenology.2021.07.013

Cited by 3 publications

(3 citation statements)

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“…Indeed, methionine is responsible for the generation of a key cofactor that is essential for the establishment of DNA-methylations. Maternal supplementation of RPM before conception and/or during the preimplantation period might alter the expression of genes associated with uterine immune responses and metabolism ( Guadagnin et al, 2021 ) probably via its epigenetic modification effects. In the current study, we compared the endometrial DNA methylation patterns of dairy cows daily supplemented with RPM and resulted in pregnancy (MET_P) compared to those that resulted in no pregnancy (MET_noP).…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, cows exhibit elevated concentrations of ketone bodies, namely beta hydroxybutyrate (ßHB) and non-esterified fatty acids (NEFA) ( Butler and Smith, 1989 ). In turn, the metabolic stress induced during the early lactation period negatively affects the cow’s fertility by altering the transcriptome profile of the maternal environment including endometrium ( Wathes et al, 2007 ; Fenwick et al, 2008 ; Wathes et al, 2009 ; Guadagnin et al, 2021 ) and follicular microenvironment ( Llewellyn et al, 2007 ; Golini et al, 2014 ; Warma et al, 2020 ) and miRNAs in follicular fluid ( Hailay et al, 2019 ). Likewise, early lactation-induced metabolic stress reduces fertility by altering the concentration of various reproductive hormones that are controlled by the hypothalamus-pituitary-ovary axis ( Beam and Butler, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

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Endometrial DNA methylation signatures during the time of breeding in relation to the pregnancy outcome in postpartum dairy cows fed a control diet or supplemented with rumen-protected methionine

Salilew-Wondim,

Tholen,

Held-Hoelker

et al. 2024

Front. Genet.

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Post calving metabolic stress reduces the fertility of high producing dairy cows possibly by altering the expression of genes in the maternal environment via epigenetic modifications. Therefore, this study was conducted to identify endometrial DNA methylation marks that can be associated with pregnancy outcomes in postpartum cows at the time of breeding. For this, twelve days post-calving, cows were either offered a control diet or supplemented daily with rumen-protected methionine. Cows showing heat 50–64 days postpartum were artificially inseminated. Endometrial cytobrush samples were collected 4–8 h after artificial insemination and classified based on the pregnancy out comes as those derived from cows that resulted in pregnancy or resulted in no pregnancy. The DNAs isolated from endometrial samples were then subject to reduced representative bisulfite sequencing for DNA methylation analysis. Results showed that in the control diet group, 1,958 differentially methylated CpG sites (DMCGs) were identified between cows that resulted in pregnancy and those that resulted in no pregnancy of which 890 DMCGs were located on chr 27: 6217254–6225600 bp. A total of 537 DMCGs were overlapped with 313 annotated genes that were involved in various pathways including signal transduction, signalling by GPCR, aldosterone synthesis and secretion. Likewise, in methionine supplemented group, 3,430 CpG sites were differentially methylated between the two cow groups of which 18.7% were located on Chr27: 6217254–6225600 bp. A total of 1,781 DMCGS were overlapped with 890 genes which involved in developmental and signalling related pathways including WNT-signalling, focal adhesion and ECM receptor interaction. Interestingly, 149 genes involved in signal transduction, axon guidance and non-integrin membrane-ECM interactions were differentially methylated between the two cow groups irrespective of their feeding regime, while 453 genes involved in axon guidance, notch signalling and collagen formation were differentially methylated between cows that received rumen protected methionine and control diet irrespective of their fertility status. Overall, this study indicated that postpartum cows that could potentially become pregnant could be distinguishable based on their endometrial DNA methylation patterns at the time of breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endometrial DNA methylation signatures during the time of breeding in relation to the pregnancy outcome in postpartum dairy cows fed a control diet or supplemented with rumen-protected methionine

Salilew-Wondim,

Tholen,

Held-Hoelker

et al. 2024

Front. Genet.

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“…Primers were chosen based on previous research demonstrating the expression of such targets involved in inflammatory and metabolic processes in the bovine reproductive tract (Brewer et al, 2020;Guadagnin et al, 2021). To control analytical and tissue sampling variation, the final data were normalized to expression of the geometric mean of GAPHD, ACTB, and H2AFZ, which were validated as suitable internal control transcripts in bovine uterine tissue (Cerri et al, 2012;Gómez et al, 2017).…”

Section: Rna Extraction and Real Time Quantitative Pcrmentioning

confidence: 99%

Effect of feeding rumen-protected lysine through the transition period on postpartum uterine health of dairy cows

Guadagnin

Fehlberg

Thomas

et al. 2022

Journal of Dairy Science

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Feeding rumen-protected methionine as an indispensable amino acid source has been shown to improve reproductive performance in dairy cows, but the effect of feeding rumen-protected lysine (RPL) during the peripartum period on reproductive performance is not well explored. Therefore, we aimed to determine the effects of feeding RPL (AjiPro-L Generation 3, Ajinomoto Heartland Inc.) prepartum, postpartum, or both on follicular dynamics, uterine health, and mRNA gene expression of the endometrium. Seventy-five multiparous Holstein cows were assigned to 1 of 2 dietary treatments with or without RPL in a randomized, complete block design. A 2 × 2 factorial arrangement of treatments was used. Prepartum (-28 d to calving), animals were fed a diet (68% of dietary DM from forage) with RPL [PRE-L; 0.54% RPL of dietary dry matter intake] or without RPL (PRE-C). After calving, half of the cows from each prepartum treatment group were assigned to a diet (56% forage) with RPL (PRE-L POST-L; PRE-C POST-L; 0.40% RPL of dietary dry matter intake) or without RPL (PRE-C POST-C; PRE-L POST-C) until 28 d in milk (DIM). Vaginal discharge was detected with a Metricheck device (Simcro) to detect metritis, and at 28 DIM polymorphonuclear leukocytes were evaluated as a percentage of the epithelial cells using a cytology brush (Andwin Scientific) and an endometrial tissue biopsy was collected for mRNA expression and histology. The first postpartum follicular growth cycle was monitored

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Feeding rumen-protected lysine altered immune and metabolic biomarkers in dairy cows during the transition period

Fehlberg

Guadagnin

Thomas

et al. 2023

Journal of Dairy Science

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Methionine supply during the peripartum period and early lactation alter immunometabolic gene expression in cytological smear and endometrial tissue of holstein cows

Cited by 3 publications

References 53 publications

Endometrial DNA methylation signatures during the time of breeding in relation to the pregnancy outcome in postpartum dairy cows fed a control diet or supplemented with rumen-protected methionine

Endometrial DNA methylation signatures during the time of breeding in relation to the pregnancy outcome in postpartum dairy cows fed a control diet or supplemented with rumen-protected methionine

Effect of feeding rumen-protected lysine through the transition period on postpartum uterine health of dairy cows

Feeding rumen-protected lysine altered immune and metabolic biomarkers in dairy cows during the transition period

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