Methionine-induced Ethylene Production by <i>Penicillium digitatum</i>

Chalutz, E.; Lieberman, Morris; Sisler, H. D.

doi:10.1104/pp.60.3.402

Cited by 72 publications

(38 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results on ethylene inhibition obtained in this study, with both the static and shake cultures of the fungus, in which ethylene is being produced from different precursors (3,4), show similarities to those obtained with higher plants (10,11). They support the hypothesis that these compounds inhibit ethylene production by irreversibly attaching to the ethylene-forming enzyme.…”

Section: Resultssupporting

confidence: 72%

“…Determination of radioactivity, inoculation and incubation procedures, culture weight determination, and ethylene analyses were performed as previously described (3,15). Further details of techniques are described in the legends to the tables.…”

Section: Methodsmentioning

confidence: 99%

“…In a recent paper (3) analogue of rhizobitoxine, inhibited production of ethylene by both static and shake cultures of P. digitatum. This observation seemed to be in contrast with a previous finding (11) that rhizobitoxine did not inhibit ethylene production by the fungus in static culture.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of Ethylene Production in Penicillium digitatum

Chalutz¹,

Lieberman²

1978

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Production of ethylene by static cultures of Penicillium digitatum, which utilize glutamate and a-ketoglutarate as ethylene precursors, was inhibited by methionine, methionine sulfoxide, methionine sulfone, and methionine sulfoximine. Rhizobitoxine did not affect ethylene production but its ethoxy and methoxy analogues were effective inhibitors of ethylene production; its saturated methoxy analogue and kainic acid stimulated ethylene production. Tracer studies showed that the inhibitors blocked the conversion of [3Hlglutamate into [3Hlethylene.In shake cultures of this fungus, which utilize methionine as the ethylene precursor, rhizobitoxine and its unsaturated analogues all inhibited, while the saturated methoxy analogue stimulated ethylene production. In both types of cultures inhibition was irreversible and was diminished by increasing concentrations of the ethylene precursor. The inhibitory activity or lack of it by rhizobitoxine and its analogues appears to be a function of their structural resemblance to glutamate and methionine as well as of their size and stereoconfiguration. These data suggest similarities between the ethylene-forming system in the fungus and in higher plants despite differences in precursors under some cultural conditions. The production of ethylene by Penicillium digitatum Sacc., the green mold fungus of citrus fruits, has been extensively studied (2,6,7,17). Cultural conditions affecting ethylene production were described (5,14,15), and the biosynthetic pathway partially elucidated (4, 6, 7). When the fungus is grown in static culture, ethylene is derived from a-ketoglutarate or glutamate (4) but not from methionine, the only known precursor of ethylene in higher plants (8,9,16 analogue of rhizobitoxine, inhibited production of ethylene by both static and shake cultures of P. digitatum. This observation seemed to be in contrast with a previous finding (11) that rhizobitoxine did not inhibit ethylene production by the fungus in static culture. To resolve this apparent contradiction and to better understand the mode of ethylene production and its control in static and shake cultures of P. digitatum, we studied the effects of ethylene substrates, substrate analogues and inhibitors on the production of ethylene by this fungus. We report the results of this study and discuss their significance. MATERIALS AND METHODSP. digitatum (ATCC No. 10030) was grown on modified Pratt's liquid medium (15). Two types of cultures, static and shake, were used in this study. Static cultures were prepared by aseptically inoculating cotton-plugged, 50-ml, Erlenmeyer flasks containing 10 ml of medium. The fungus was grown statically for 4 days and the medium underneath the mycelial mat was then poured out of the flask and replaced with solutions of the chemicals tested at pH 4.5. Shake cultures were grown in 250-ml Erlenmeyer flasks containing 50 ml of medium. These flasks were incubated on a shaker as previously described (15). After 4 days of growth, the cultures were centrifuged, and portions (0....

show abstract

Section: Resultssupporting

confidence: 72%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of Ethylene Production in Penicillium digitatum

Chalutz¹,

Lieberman²

1978

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…8 and 9) with the exception of the two hiah levels of gylcerol in Soil L. These results are in contrast to those reported by Hunt et al (1980) and Sutherland and Cook (1980) who reported that the addition of methionine had no effect on the oroduction of C 2 H 4 in their soils. Several other reports, however, agree that methionine sicmificantly increased C 2 H 4 production (Chalutz and Lieberman, 1977;Lynch. 1972;Lynch and Harper,1974).…”

Section: Control Me1hioninementioning

confidence: 83%

Microbial production of ethylene in desert soils

Babiker¹,

Pepper²

1984

Soil Biology and Biochemistry

View full text Add to dashboard Cite

“…L-Methionine has been established as a precursor of ethylene in higher plants and several microorganisms (3,13,20). In contrast, an incubation of P. tricornutum with L-methionine (100 mm or 10 mm final concentration) for 24 h has no influence on the production of ethylene or the other hydrocarbons.…”

mentioning

confidence: 99%

Production of Hexanal and Ethane by Phaeodactylum triconutum and Its Correlation to Fatty Acid Oxidation and Bleaching of Photosynthetic Pigments

Schobert¹,

Elstner²

1980

Plant Physiol.

View full text Add to dashboard Cite

MATERIALS AND METHODSIn a Ught-dependent reaction (3.5 kilolux) at pH 5, the evolution of hexanal, ethane, and ethylene has been established with cell suspensions of the diatom, Phaeodactylwn ticornutum During this process, chlorophyll and carotenoids are partially bleached. Addition of 25 milUmolar a-Unolenic acid or 12 millimolar docosahexaenoic acid yield total pigment destruction and enhancement of ethylene and ethane formation (by about 150 and 7,600%, respectively), whereas hexanal production decreases by 70%. Eicosapentaenoic acid, the major polyunsaturated fatty acid in diatoms, stimulates both ethane and hexanal formation (by about 1,400 and 130%, respectively), but reduces ethylene production (by about 60%). This competition suggests that the production of the volatile compounds is closely connected, although hexanal and ethylene obviously possess different unsaturated fatty acids as precursors. Both the kind of the fatty acids and their relative amounts seem to determine the pattern of the evolved hydrocarbons. The presence of 10 millimolar propylgallate inhibits the evolution of the volatile compounds by about 80%, indicating that radical formation might play a key role in this light-dependent cascade of reactions.

show abstract

Methionine-induced Ethylene Production by Penicillium digitatum

Cited by 72 publications

References 11 publications

Inhibition of Ethylene Production in Penicillium digitatum

Inhibition of Ethylene Production in Penicillium digitatum

Microbial production of ethylene in desert soils

Production of Hexanal and Ethane by Phaeodactylum triconutum and Its Correlation to Fatty Acid Oxidation and Bleaching of Photosynthetic Pigments

Contact Info

Product

Resources

About