Methionine deficiency reduces autophagy and accelerates death in intestinal epithelial cells infected with enterotoxigenic Escherichia coli

Abstract

Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without L-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy.

Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

“…Bama mini-pigs had higher (P < 0.05) mRNA levels for LAT1, SNAT2, and EAAC1, but a lower (P < 0.05) mRNA level for PepT1, compared with Landrace pigs. Collectively, our synthesis in cells and signaling molecules (Wu et al , 2012(Wu et al , b, 2015Li et al 2011;Wang et al 2014aWang et al , b, 2015a but also regulators of gene expression (He et al 2013;Chen et al 2014;Zhang et al 2013;Ren et al 2013aRen et al , b, c, 2014aTan et al 2011;Yang et al 2013;Feng et al 2014;Tang et al 2014Kong et al 2014) and protein posttranslational regulation . Additionally, AAs are key precursors for the biosynthesis of hormones and low-molecular-weight nitrogenous substances (Wu 2013a, b).…”

On the Formation of Amide Polymers via Carbonyl–amino Group Linkages in Energetically Processed Ices of Astrophysical Relevance

“…Additionally, more than 80 amino acids were identified in meteorites such as Murcheson and the Lonewolf Nunataks 94102 (Kvenvolden et al 1970;Cronin & Chang 1993;Glavin et al 2006;Elsila et al 2007). A detailed isotopic analysis (D/H; N) provided compelling evidence that these molecules were formed in the ISM (Robert & Epstein 1982;Yang & Epstein 1983;Cronin & Chang 1993;Alexander et al 1998). However, with the exception of formamide (HCONH 2 ; Jones et al 2011), the underlying formation mechanisms of the COM carrying the peptide bond are far from being understood.…”

Dysfunction of methionine sulfoxide reductases to repair damaged proteins by nickel nanoparticles

“…Previous reports have shown MSRA and MSRB were involved in the regulation of mitochondrial function [29], leading to alterations in autophagy function [30]. Furthermore, Tang and colleagues observed that MSR reduction caused autophagy inactivation and accelerated cell death [30].…”