Methionine aminopeptidase‑2 is a pivotal regulator of vasculogenic mimicry

Shimizu, Shota; Kawahara, Ryota; Simizu, Siro

doi:10.3892/or.2021.8242

Cited by 8 publications

(4 citation statements)

References 43 publications

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“…Therefore, the classical base paring between the seed region of miRNAs and the mRNA target site might not be the sole cause for target gene selection, and higher order structures of miRNAs could also modulate mRNA targeting, in addition or even independently of the base composition in the seed region. Increase of vascular mimicry [56,57] Beta-1,3-Nacetylglucosaminyltransferasee…”

Section: Discussionmentioning

confidence: 99%

Artificial miRNAs derived from miR‐181 family members have potential in cancer therapy due to an altered spectrum of target mRNAs

et al. 2023

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miRNAs are a class of noncoding RNAs with gene regulation properties, and they function as key factors in cell homeostasis. The interaction of miRNAs with their target mRNAs is largely considered to rely on sequence complementarity; however, some evidence indicates that mature miRNAs can adopt diverse conformations with implications for their function. Using the oncogenic miR‐181 family as a study model, we suggest that a potential relationship between the primary sequence and secondary structure of miRNAs may have an impact on the number and spectrum of targeted cellular transcripts. We further emphasize that specific alterations in miR‐181 primary sequences might impose certain constraints on target gene selection compared with the wild‐type sequences, leading to the targeting of new transcripts with upregulated function in cancer.

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Section: Discussionmentioning

confidence: 99%

Artificial miRNAs derived from miR‐181 family members have potential in cancer therapy due to an altered spectrum of target mRNAs

et al. 2023

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“…Establishment of DPY19L3-knockout cell lines using the CRISPR/Cas9 system DPY19L3-knockout (KO) HT1080 cell line was formed with the CRISPR/Cas9 system using formerly reported methods [18,19]. The oligos that were used to create single-guide RNA (sgRNA) were inserted into the BbsI site of the pSpCas9n(BB)-2A-Puro (PX459) V2.0 vector, which was placed into Addgene by Dr. Feng Zhang (#62987, Addgene, Cambridge, MA, USA).…”

Section: Cell Culturementioning

confidence: 99%

DPY19L3 promotes vasculogenic mimicry by its C-mannosyltransferase activity

BAYDOUN,

KATO,

KAMO

et al. 2024

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C -mannosylation is a post-translational modification that occurs intracellularly in the endoplasmic reticulum. In humans, biosynthesis of C -mannosylation in proteins containing thrombospondin type 1 repeat is catalyzed by the DPY19 family; nonetheless, biological functions of protein C -mannosylation are not yet fully understood, especially in tumor progression. Vasculogenic mimicry (VM) is the formation of fluid-conducting channels by highly invasive and genetically deregulated tumor cells, enabling the tumors to form matrix-embedded vasculogenic structures, containing plasma and blood cells to meet the metabolic demands of rapidly growing tumors. In this study, we focused on DPY19L3, a C -mannosyltransferase, and aimed to unravel its role in VM. Knockout of DPY19L3 inhibited the formation of VM in HT1080 human fibrosarcoma cells. Re-expression of wild-type DPY19L3 recovered VM formation; however, DPY19L3 isoform2, an enzymatic activity-defect mutant, did not restore it, suggesting that the C -mannosyltransferase activity of DPY19L3 is crucial to its function. Furthermore, the knockdown of DPY19L3 in MDA-MB-231 breast cancer cells hindered its network formation ability. Altogether, our findings suggest that DPY19L3 is required for VM formation and stipulate the relevance of C -mannosylation in oncogenesis.

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“…VM assay was performed as previously described [25,[29][30][31][32]. Briefly, 96-well plates were coated with 40 lL per well of Matrigel Ò Growth Factor Reduced (Corning, Corning, NY, USA) and incubated for 30 min at 37 °C.…”

Section: Vm Assaymentioning

confidence: 99%

Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells

2023

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Vasculogenic mimicry (VM) is the formation of microvascular channels by cancer cells. VM requires cellular processes that are regulated by changes in cellular migration and morphology. Cofilin (CFL), a key regulator of actin depolymerization, has been reported to affect malignant phenotypes of cancer. We show that treatment with inhibitors of actin dynamics suppresses VM in MDA-MB-231 human breast cancer cells. We established CFL-knockout (KO) MDA-MB-231 cells and found that VM was attenuated in CFL-KO cells. Although the re-expression of wild-type CFL restored VM in CFL-KO cells, inactive phosphomimetic CFL failed to do so. Collectively, our results demonstrate that CFL is a critical regulator of VM and implicate CFL as a novel therapeutic target for breast cancer.

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Methionine aminopeptidase‑2 is a pivotal regulator of vasculogenic mimicry

Cited by 8 publications

References 43 publications

Artificial miRNAs derived from miR‐181 family members have potential in cancer therapy due to an altered spectrum of target mRNAs

Artificial miRNAs derived from miR‐181 family members have potential in cancer therapy due to an altered spectrum of target mRNAs

DPY19L3 promotes vasculogenic mimicry by its C-mannosyltransferase activity

Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells

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