Methicillin-resistant Staphylococcus aureus sequence type 45 with high rates of ciprofloxacin and tetracycline resistance in the residents and environments of long-term care facilities in Taiwan

Lai, Chih Cheng; Lee, Chun Ming; Chiang, Hsiu Tzy; Lü, Min; Wang, Ling Fang; Tsai, Tsai Ling; Kang, Mei; Jan, Yi Ni; Lo, Yi Ting; Ko, Wen Chien; Tseng, Sheng-Hong; Hsueh, Po-Ren

doi:10.1016/j.jinf.2017.11.003

Cited by 10 publications

(4 citation statements)

References 9 publications

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“…aureus is a common pathogen that can cause post-partum clinical and subclinical endometritis in humans and animals (Turner et al 2012;Zhang et al 2017). During the long-term use of antibiotics, S. aureus has been found to develop resistance (Hiramatsu 2001;Lai et al 2018;Pirolo et al 2019). To (Koehbach 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Staphylococcus aureus is a facultative pathogen that can cause myriad infectious diseases in humans and animals (Fluit 2012;Foster 2012;Lai et al 2018;Tong et al 2015), including endometritis, a common reproductive disease (Sheldon and Owens 2017), and severely impaired reproductive performance (Gilbert et al 2005); if not controlled, it may promote the development of septicemia and sepsis (Skovbakke and Franzyk 2017). It is estimated that 340 million women get bacterial infections in the uteri each year, and 15~20% animals develop clinical or subclinical endometritis beyond 3 weeks post-partum, costing billion dollars for treatments annually (Turner et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Yang

Shan

et al. 2020

Appl Microbiol Biotechnol

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Staphylococcus aureus is a common pathogen that can cause clinical and subclinical endometritis in humans and animals. In this study, a designed CSαβ peptide ID13 from DLP4 exhibited high stable antibacterial activity in simulated gastric fluid (90.79%), serum (99.54%), and different pH buffers (> 99%) against S. aureus CVCC 546 and lower cytotoxicity (89.62% viability) than its parent peptide DLP4 (74.14% viability) toward mouse endometrial epithelial cells (MEECs). ID13 caused a depolarization of bacterial membrane and downregulation of the expression of genes involved in membrane potential maintenance and biofilm formation. The in vitro efficacy analysis of ID13 showed a synergistic effect with vancomycin, ampicillin, rifampin, and ciprofloxacin; intracellular antimicrobial activity against S. aureus CVCC 546 in MEECs; and the ability to inhibit lipoteichoic acid-induced pro-inflammatory cytokines from RAW 264.7. In the S. aureus-induced endometritis of mice, similar to vancomycin, ID13 remarkably alleviated pathological conditions, inhibited the production of cytokines (TNF-α, IL-1ß, IL-6, and IL-10), and suppressed the TLR2-NF-κB signal pathway. Collectively, these results suggest that ID13 could be a potential candidate peptide for therapeutic application in S. aureus-induced endometritis. Key Points•Higher antibacterial activity and lower hemolysis of ID13 than DLP4. •ID13 could downregulate the genes of bacterial survival and infection.•ID13 could alleviate the S. aureus-induced endometritis of mice.•ID13 could regulate the cytokines and suppress the TLR2-NF-κB signal pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Yang

Shan

et al. 2020

Appl Microbiol Biotechnol

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“…More recently, in a longitudinal survey (bimonthly for 6 months with four surveys) for MRSA carriage amongst residents and HCWs in four nursing homes in northern Taiwan between August 2016 and February 2017 (unpublished data), we found that 40 % of the participants had MRSA carriage and again ST45 accounted for nearly half of the 172 MRSA isolates. In two other surveys for MRSA colonization among residents and environments conducted in six nursing homes in 2015 and 2016, respectively, MRSA ST45 accounted for nearly 30 and 20 % of the MRSA isolates, respectively [ 40, 41 ]. These findings suggest that the MRSA ST45 strain has prevailed in nursing homes in Taiwan since 2012 or probably earlier.…”

Section: Discussionmentioning

confidence: 99%

Detection, spread and phylogeny of meticillin-resistant Staphylococcus aureus sequence type 45 in Taiwan

Huang

Chen

Lauderdale³

2021

Microbial Genomics

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Meticillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 45 was reported in the literature to have been first identified in 2006 in Taiwan. The present study was carried out to explore and trace the emergence, transmission and evolutional dynamics of MRSA ST45 in Taiwan. We identified MRSA ST45 isolates retrospectively from two collections of MRSA isolates, namely TSAR (Taiwan Surveillance of Antimicrobial Resistance) surveys and the CGMH (Chang Gung Memorial Hospital)-based laboratory collection. Representative ST45 isolates were selected for whole-genome sequencing (WGS) analysis. A total of 9554 MRSA isolates was included in this study. Among the 3766 MRSA isolates biennially collected from TSAR surveys between 1998 and 2014, ST45 accounted for 133 (3.53 %) MRSA isolates, was first identified in 2004, and the prevalence rate peaked in 2010 (up to 10.77 %). Among the 5788 MRSA isolates collected between 1995 and 2017 by the CGMH-based laboratory, 257 isolates (4.44 %) were characterized as ST45, with most identified from nursing homes since 2012. Of the 75 isolates randomly selected for WGS, two clades were identified. The major clade, clade II, comprised 63 isolates and was phylogenetically relatively close to those isolates identified from Singapore. All but one of the isolates in clade I, the minor clade, were identified from non-Taiwanese people, mostly from newly recruited foreign workers in 2017, and were phylogenetically relatively close to one isolate from the USA (CA-347). Conclusively, the emergence of MRSA ST45 strain in Taiwan can be traced back to 2004 and the strain is connected to South-East Asian countries. Since its emergence, transmission and spread of MRSA ST45 has occurred in Taiwan.

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“…Moreover, Roch et al (2017) have described a clinical case with MRSA infection in which the strain isolated was resistant to daptomycin (Roch et al, 2017). Indeed, MRSA have not developed resistance only to daptomycin but also to tetracycline and ciprofloxacin (Lai et al, 2017).…”

Section: Anti Anti Antimentioning

confidence: 99%

From skin infection to invasive diseases: A descriptive review of Staphylococcus aureus, focusing on Panton-Valentine leucocidin and methicillin-resistant strains

ZEOUK¹

2023

Not Sci Biol

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Despite advances in scientific research, Staphylococccus aureus remains a pyogenic and toxigenic bacterium involved in different infections, it endowed with the capacity to infect several biotopes and cause a wide range of infections ranging from skin diseases to other serious pathologies such as pneumonia, meningitis, sepsis, osteomyelitis and infectious endocarditis. Moreover, the emergence of resistant strains constitutes a serious public health problem. Thus, the development of new active compounds from natural sources such as medicinal plants is urgently needed. To this end, the aim of our review was to describe the state of art of infections caused by S. aureus, its pathogenesis, treatment and to provide a synthesis about studies reporting a bio guided isolation of most promising compounds selected for their anti-staphylococcal activity.

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Methicillin-resistant Staphylococcus aureus sequence type 45 with high rates of ciprofloxacin and tetracycline resistance in the residents and environments of long-term care facilities in Taiwan

Cited by 10 publications

References 9 publications

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Detection, spread and phylogeny of meticillin-resistant Staphylococcus aureus sequence type 45 in Taiwan

From skin infection to invasive diseases: A descriptive review of Staphylococcus aureus, focusing on Panton-Valentine leucocidin and methicillin-resistant strains

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