Methicillin-resistant Staphylococcus aureus antibiotic susceptibility profile and associated factors among hospitalized patients at Hawassa University Comprehensive Specialized Hospital, Ethiopia

Teka, Freezer; Alemayehu, Tsegaye; Ali, Musa Mohammed

doi:10.1016/j.ijregi.2022.03.015

Cited by 7 publications

(7 citation statements)

References 21 publications

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“…In the present study methicillin resistant S.aureus was frequently isolated from pus and discharges and blood culture. The rate of methicillin resistant S. aureus, 30% in the present study is comparable to 28.3% rate of MRSA isolation from Desie hospital, North West Ethiopia [10], but lower compared to the national pooled rate 32.5% [6], 68% reported from Awasa, southern Ethiopia [11] and 35.6% from Tikur Anbesa Hospital Addis Ababa, Ethiopia [12]. However 30% methicillin resistant rate of S.aureus in the present study is higher than 23% methicillin resistance reported from Jimma, South west Ethiopia [13], 13.2% from Debrmarkos referral hospital, north west Ethiopia [14].…”

Section: Discussionsupporting

confidence: 50%

Isolation and Identification of Methicillin Resistant Staphylococci at Bethzatha Advanced Laboratory, Addis Ababa, Ethiopia

2024

PDRAJ

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Methicillin resistant Staphylococcus aureus and coagulase negative staphylococci (CoNS) are widely spreading. So identification and antimicrobial susceptibility tests of methicillin resistant S. aureus and coagulase negative staphylococcus (CoNS) is critical to appropriately treat patients and control the increasing spread of these pathogens. Therefore the aim of the present work is to determine methicillin resistant S.aureus and CoNS from clinical samples sent to BethZatha Advanced Laboratory from different health institutions and Bethzatha Hospital, Addis Ababa, for bacterial isolation, identification and antimicrobial susceptibility. Bacterial isolation, identification and antimicrobial susceptibility testing were done using Microscan panel methods (Beckman Coulter, Brea, CA, USA) from May 2021 to February 2022 at Bethzatha advanced Medical Laboratory. The retrospective data was analysed to determine methicillin susceptibility of S.aureus and Coagulase negative staphylococci (CoNS). The finding shows 35% (40/114) S. aureus and 65.8% (75/114) coagulase negative staphylococci (CoNS) were isolated from different clinical specimens (urine, blood culture, pus and discharges). Out of 40 S. aureus isolates 30% (12/40) were methicillin resistant. Similarly, out of 75 CoNS, 52% (39/75) were methicillin resistant. Almost all methicillin resistant S. aureus and most CoNS isolates were multiply resistant to amoxicillin clavulnate, azithromycin, penicillin and ampicillin. Periodic surveillance and appropriate control measures and safety precautions of nosocomial and community acquired methicillin resistant multidrug resistant, staphylococcus aureus, and CoNS must be given prior attention.

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Section: Discussionsupporting

confidence: 50%

Isolation and Identification of Methicillin Resistant Staphylococci at Bethzatha Advanced Laboratory, Addis Ababa, Ethiopia

2024

PDRAJ

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“…The antibiotic sensitivity result of this study showed that MRSA isolates were susceptible to antibiotics like trimethoprim-sulpha (87.5%), gentamycin (87.5%), erythromycin (75%), chloramphenicol (62.5%), and ciprofloxacin (62.5%), which is in line with studies conducted by 53 in Assam, India, and 54 in Italy. In contrast, a study from Ethiopia 29 found a high level of trimethoprim-sulfamethoxazole resistance (76.9%) in MRSA isolates. The variation in the resistance profile of the bacteria may be due to differences in drug availability and the diversity of the source isolates.…”

Section: Discussionmentioning

confidence: 77%

“…In Ethiopia, the prevalence of MRSA has been reported in different regions, which implies an increasing spread of this multidrugresistant pathogen in humans and animals. 29,43 This study assessed the occurrence of S. aureus and MRSA on 50 dairy farms. Overall, 100 samples containing 50 bulk milk samples and 50 hand swabs were evaluated for methicillin resistance by cefoxitin disk diffusion testing and mecA-specific PCR.…”

Section: Discussionmentioning

confidence: 99%

“…27,28 Several studies have been carried out in Ethiopia to investigate MRSA infections in different regions of the country. [29][30][31][32][33][34][35][36] However, there is a lack of information regarding MRSA in raw bulk milk and among farm workers. Thus, the aim of this study was to detect MRSA strains from bulk milk and dairy milkers and determine their phenotypic and genotypic antimicrobial profiles.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of Methicillin-Resistant Staphylococcus aureus (MRSA) in Bovine Bulk Milk and Farm Workers in Smallholder Dairy Farming Systems in Northwestern Ethiopia

Kassa,

Belete,

Yihunie

et al. 2024

VMRR

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Background and Purpose: Staphylococcus aureus is a common pathogen responsible for causing various human and animal infections and is well known for its ability to develop resistance to multiple antibiotics. This study aimed to evaluate the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) in bulk milk and dairy farms in northwestern Ethiopia and to determine their phenotypic and genotypic antimicrobial susceptibility patterns. Methods: We collected 50 bulk milk samples from 50 dairy farms and 50 hand swabs from dairy milkers. The cefoxitin disk diffusion test and PCR-based assays were used to identify MRSA isolates. In addition, cefoxitin-resistant isolates were tested for susceptibility to other antibiotics using the Kirby-Bauer disk diffusion method. Results: The results showed that MRSA was detected in 8 samples: 6 from bulk milk samples (12%) and 2 from hand swabs (4%). All MRSA isolates exhibited a high resistance rate to penicillin (100%), followed by tetracycline (75%), ciprofloxacin (25%), chloramphenicol (25%), erythromycin (25%), gentamycin (12.5%), and trimethoprim-sulfamethoxazole (12.5%). Moreover, 72% of the isolates showed resistance to three or more antibiotic classes and were classified as multidrug-resistant. Conclusion:This study identified methicillin-resistant Staphylococcus aureus and multidrug-resistant MRSA in bulk milk and dairy farms in northwestern Ethiopia. These findings highlight the potential risk of transmission of these antibiotic-resistant bacteria to humans and the need for improved antibiotic stewardship in the dairy sector using the One Health approach.

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“…The occurrence rates of S. aureus and MRSA contamination in raw bovine milk have been widely reported and potentially threaten human health. In Ethiopia, the prevalence of MRSA has been reported in different regions, which implies an increasing spread of this multidrug-resistant pathogen in humans and animals [29,43] This study assessed the occurrence of S. aureus and MRSA on 50 dairy farms. Overall, 100 samples containing 50 raw milk samples and 50 hand swabs were evaluated for methicillin resistance by cefoxitin disk diffusion testing and mecA-speci c PCR.…”

Section: Discussionmentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus (MRSA) in Bovine pooled milk and Close human contacts from Northwestern Ethiopia

Yesigat,

Belete,

Bizuayehu

et al. 2023

Preprint

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Staphylococcus aureus is a common pathogen responsible for causing various human and animal infections, and it is well known for its ability to develop resistance to multiple antibiotics. This study evaluated the occurrence of methicillin-resistant and methicillin-susceptible Staphylococcus aureus in dairy farms in northwestern Ethiopia. We collected 50 pooled milk samples from 50 dairy farms and 50 nasal swabs from 50 humans in close contact with animals. The cefoxitin disk diffusion test and PCR-based assays were used to identify methicillin-resistant S. aureus isolates. In addition, cefoxitin-resistant isolates were tested for susceptibility to other antibiotics using the Kirby-Bauer disk diffusion method. MRSA was detected in 8 out of the 100 individual samples tested. Out of 8 MRSA isolates, 6 were obtained from pooled milk samples, and two were from personal hand swabs. On the other hand, MSSA isolates were detected in six of the specimens. All eight MRSA isolates exhibited high percentages of resistance to cefoxitin (100%), penicillin (100%), and tetracycline (75%). Moreover, 72% of isolates showed resistance to three or more antibiotic agents. This study was the first to document the presence of methicillin-resistant Staphylococcus aureus and multidrug-resistant MRSA in raw milk and farm workers in northwestern Ethiopia.

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Methicillin-resistant Staphylococcus aureus antibiotic susceptibility profile and associated factors among hospitalized patients at Hawassa University Comprehensive Specialized Hospital, Ethiopia

Cited by 7 publications

References 21 publications

Isolation and Identification of Methicillin Resistant Staphylococci at Bethzatha Advanced Laboratory, Addis Ababa, Ethiopia

Isolation and Identification of Methicillin Resistant Staphylococci at Bethzatha Advanced Laboratory, Addis Ababa, Ethiopia

Occurrence of Methicillin-Resistant Staphylococcus aureus (MRSA) in Bovine Bulk Milk and Farm Workers in Smallholder Dairy Farming Systems in Northwestern Ethiopia

Methicillin-resistant Staphylococcus aureus (MRSA) in Bovine pooled milk and Close human contacts from Northwestern Ethiopia

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