Methicillin-resistant Staphylococcus aureus among urban rodents, house shrews, and patients in Guangzhou, Southern China

Ge, Jun; Zhong, Xiaomei; Xiong, Yiquan; Qiu, Min; Huo, Shuting; Chen, Xue-Jiao; Mo, Yun; Cheng, Mingji; Chen, Qing

doi:10.1186/s12917-019-2012-8

Cited by 17 publications

(14 citation statements)

References 51 publications

(51 reference statements)

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“…The lukS/F-PV virulence gene was rarely reported in S. aureus of wild animals; however, five studies detected PVL-positive MRSA and MSSA isolates from NHP and WR [ 39 , 42 , 43 , 46 , 47 ]. Interestingly, most of the PVL-positive isolates were detected in MSSA or MRSA in NHP [ 42 , 43 , 46 , 47 ], although there is one study performed on urban rodents in China which detected the PVL-positive-MRSA-CC398-t034 isolate [ 39 ]. It is worthy to mention that MRSA strains in rats in contact with cattle can be colonised by LA-MRSA [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Of the 33 eligible and analysed studies ( Figure 1 ), 6, 3, 2 and 22 were from Africa, America, Asia and Europe, respectively [ 5 , 12 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]; none were reported in Australia and the Pacific regions. Supplementary Table S1 shows the characteristics and data of the 33 eligible studies, with the indication of the country, type of animals (divided into four groups: wild mammals (WM), non-human primates (NHP), wild birds (WB) and wild rodents (WR)), number of animals tested, number of S. aureus and MRSA obtained and the AMR and virulence profiles.…”

Section: Main Findingsmentioning

confidence: 99%

“…Most MRSA-CC398 isolates showed tetracycline resistance (80%). Nevertheless, three MRSA-CC398 isolates obtained from wild rodents in China were tetracycline-susceptible ( spa -types t4652 and t2582) and, interestingly, one isolate of this study carried the genes of the Panton-Valentine Leukocidin ( spa -t034 and IEC-type E) [ 39 ]. In relation to the antibiotic resistance profile of the MSSA-CC398 isolates, this was determined in 16 of these isolates and erythromycin resistance was found in 10 isolates carrying the ermT gene in 9 of them.…”

Section: Main Findingsmentioning

confidence: 99%

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Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern

et al. 2021

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Background: The availability of comprehensive data on the ecology and molecular epidemiology of Staphylococcus aureus/MRSA in wild animals is necessary to understand their relevance in the “One Health” domain. Objective: In this study, we determined the pooled prevalence of nasal, tracheal and/or oral (NTO) Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) carriage in wild animals, with a special focus on mecA and mecC genes as well as the frequency of MRSA and methicillin susceptible S. aureus (MSSA) of the lineages CC398 and CC130 in wild animals. Methodology: This systematic review was executed on cross-sectional studies that reported S. aureus and MRSA in the NTO cavities of wild animals distributed in four groups: non-human primates (NHP), wild mammals (WM, excluding rodents and NHP), wild birds (WB) and wild rodents (WR). Appropriate and eligible articles published (in English) between 1 January 2011 to 30 August 2021 were searched for from PubMed, Scopus, Google Scholar, SciElo and Web of Science. Results: Of the 33 eligible and analysed studies, the pooled prevalence of NTO S. aureus and MRSA carriage was 18.5% (range: 0–100%) and 2.1% (range: 0.0–63.9%), respectively. The pooled prevalence of S. aureus/MRSA in WM, NHP, WB and WR groups was 15.8/1.6, 32.9/2.0, 10.3/3.4 and 24.2/3.4%, respectively. The prevalence of mecC-MRSA among WM/NHP/WB/WR was 1.64/0.0/2.1/0.59%, respectively, representing 89.9/0.0/59.1/25.0% of total MRSA detected in these groups of animals.The MRSA-CC398 and MRSA-CC130 lineages were most prevalent in wild birds (0.64 and 2.07%, respectively); none of these lineages were reported in NHP studies. The MRSA-CC398 (mainly of spa-type t011, 53%), MRSA-CC130 (mainly of spa types t843 and t1535, 73%), MSSA-CC398 (spa-types t571, t1451, t6606 and t034) and MSSA-CC130 (spa types t843, t1535, t3625 and t3256) lineages were mostly reported. Conclusion: Although the global prevalence of MRSA is low in wild animals, mecC-mediated resistance was particularly prevalent among MRSA isolates, especially among WM and WB. Considering the genetic diversity of MRSA in wild animals, they need to be monitored for effective control of the spread of antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Main Findingsmentioning

confidence: 99%

Section: Main Findingsmentioning

confidence: 99%

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Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern

et al. 2021

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“…Multidrug-resistant bacteria have increased worldwide, resulting in the sharing of their genes with commensal microorganisms in humans, animals, and the environment and endangering public health [12]. Rodents have been extensively documented to carry and transmit different zoonotic pathogens, including S. aureus, to humans and livestock [3,13,14]. Commensal rodents colonized with pathogens have been widely reported to invade chicken [15,16] and human houses [17][18][19], exposing them to bacterial infections.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Multidrug-Resistant Staphylococcus aureus among Humans, Rodents, Chickens, and Household Soils in Karatu, Northern Tanzania

Sonola¹,

Misinzo

Matee

2021

IJERPH

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We conducted this study to investigate the isolation frequency and phenotypic antibiotic resistance pattern of Staphylococcus aureus isolated from rodents, chickens, humans, and household soils. Specimens were plated onto mannitol salt agar (Oxoid, Basingstoke, UK) and incubated aerobically at 37 °C for 24 h. Presumptive colonies of S. aureus were subjected to Gram staining, as well as catalase, deoxyribonuclease (DNAse), and coagulase tests for identification. Antibiotic susceptibility testing was performed by using the Kirby–Bauer disc diffusion method on Mueller–Hinton agar (Oxoid, Basingstoke, UK). The antibiotics tested were tetracycline (30 μg), erythromycin (15 μg), gentamicin (10 μg), ciprofloxacin (5 μg), clindamycin (2 μg), and amoxicillin-clavulanate (20 μg/10 μg). The S. aureus strain American Type Culture Collection (ATCC) 25,923 was used as the standard organism. We found that 483 out of 956 (50.2%) samples were positive for S. aureus. The isolation frequencies varied significantly between samples sources, being 52.1%, 66.5%, 74.3%, and 24.5%, respectively, in chickens, humans, rodents, and soil samples (p < 0.001). S. aureus isolates had high resistance against clindamycin (51.0%), erythromycin (50.9%), and tetracycline (62.5%). The overall prevalence of multidrug-resistant (MDR) S. aureus isolates was 30.2%, with 8.7% resistant to at least four different classes of antibiotics.

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“…Over the years, staphylococci and AMR have been extensively investigated in humans and domestic animals, but there are limited studies on wild and pest species, particularly in developing countries [8,9]. It is known that some pest species can act as reservoirs for antimicrobial-resistant bacteria, transmitting and disseminating these microorganisms by different routes, including feces and urine [10].…”

Section: Introductionmentioning

confidence: 99%

Clostridioides difficile and multi-drug-resistant staphylococci in free-living rodents and marsupials in parks of Belo Horizonte, Brazil

et al. 2021

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The global emergence of antimicrobial resistance (AMR) has become a serious threat to human and animal health. Recent studies have shown that synanthropic animals can act as reservoirs and disseminators of pathogens and resistant bacteria. The aim of this study was to evaluate the frequency, distribution, and antimicrobial susceptibility of staphylococcal species and Clostridioides difficile isolated from the feces of free-living rodents and marsupials from two urban parks in Belo Horizonte, Brazil. During a 12-month period, fecal samples from 159 free-living animals, including 136 rodents and 23 marsupials, were collected from two urban parks in Belo Horizonte, Minas Gerais, Brazil. Staphylococcus spp. were more likely to be isolated from rodents than marsupials (p = 0.0164). Eight different staphylococcal species were isolated from 36 (26.5%) rodents and one marsupial (4.3%). S. saprophyticus (48.6%) was the most frequently isolated species, and almost a quarter of the isolates (24.3%) were resistant to at least one antimicrobial agent, four (10.8%) of which were multi-drug resistant (MDR). Two (5.4%) strains were resistant to cefoxitin and were then classified as methicillin-resistant staphylococci, and one also tested positive for the mecA gene. C. difficile was isolated from two rodents (1.5%), and one strain was toxigenic and classified as ribotype 064. One isolate was resistant to rifampicin, but both strains were susceptible to all other antimicrobials tested, including metronidazole and vancomycin. All C. difficile isolates and all staphylococcal strains resistant to antimicrobials were recovered from the same park. The present study suggests that free-living rodents in Belo Horizonte (Brazil) are mainly colonized by S. saprophyticus and may act as reservoirs of antimicrobial-resistant Staphylococcus spp. and C. difficile strains. This is the first study to evaluate the presence of staphylococci and C. difficile from free-living opossums and suggest a low fecal shedding of these organisms by these mammals.

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Methicillin-resistant Staphylococcus aureus among urban rodents, house shrews, and patients in Guangzhou, Southern China

Cited by 17 publications

References 51 publications

Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern

Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern

Occurrence of Multidrug-Resistant Staphylococcus aureus among Humans, Rodents, Chickens, and Household Soils in Karatu, Northern Tanzania

Clostridioides difficile and multi-drug-resistant staphylococci in free-living rodents and marsupials in parks of Belo Horizonte, Brazil

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