Methanotrophic symbiont location and fate of carbon incorporated from methane in a hydrocarbon seep mussel

Streams, Megan E.; Fisher, Charles R.; Fiala-Médioni, Aline

doi:10.1007/s002270050187

Cited by 71 publications

(65 citation statements)

References 36 publications

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“…by Fisher and Childress (1992) who detected an increase of radiolabel coming from 14 CH 4 in symbiont-free tissues during a chase period. Evidence for intracellular digestion of symbionts in the same mussel species was provided later by Streams et al (1997). Partially degraded bacteria in bacteriocyte bodies with lysosomal activity were also observed in B. azoricus , indicating that this could be the mode of carbon transfer from the symbiont to the host.…”

Section: Discussionmentioning

confidence: 78%

Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus

et al. 2008

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Abstract. High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide-and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 and 39% of total bacterial area and both symbionts developing according to the presence or absence of their substrates. Using H 13 CO − 3 in the presence of sulphide, or 13 CH 4 , we monitored carbon assimilation by the endosymbionts and its translocation to symbiont-free mussel tissues. Carbon was incorporated from methane and sulphide-oxidized inorganic carbon at rates 3 to 10 times slower in the host muscle tissue than in the symbiontcontaining gill tissue. Both symbionts thus contribute actively to B. azoricus nutrition and adapt to the availability of their substrates. Further experiments with varying substrate concentrations using the same set-up should provide useful tools to study and even model the effects of changes in hydrothermal fluids on B. azoricus' chemosynthetic nutrition.

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Section: Discussionmentioning

confidence: 78%

Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus

et al. 2008

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“…There are several other examples of correlative lipid metabolism and expansion of Golgi in the literature. Hypertrophy of the Golgi apparatus and consistent localization of acid phosphatase, particularly in the TGN in gill cells, have been detected during digestion of methanotrophic symbionts in hydrocarbon seep mussels (Streams et al, 1997). These findings in invertebrates are substantiated by data in mammals in which biliary secretion and upregulated lipid transport are linked with vesicle production off the Golgi complex.…”

Section: Discussionmentioning

confidence: 72%

In vivo interaction of pulmonary intravascular macrophages with activated platelets in microvessels of equine lung after multiple exposures to halothane, isoflurane, and thiamylal: A comparative ultrastructural and cytochemical study

Atwal

McDonell

2005

Anat. Rec.

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The pulmonary intravascular macrophages (PIMs) of equines contain a unique electron-dense surface coat that is predominantly composed of lipoproteins. A single exposure of inhalatory halothane causes mobilization of the surface coat into the endocytotic system of the PIMs, followed by expansion of the Golgi apparatus and its enrichment with acid phosphatase. Simultaneously, the cells of the lymphocytic series show hyperplasia in the form of mitotic changes inside the microvascular compartment of the lung. Halothane is known to cause acute and chronic hepatotoxicity because of its biotransformation into trifluoroacytelated polypeptides. The present study was designed to examine the comparative effects of reexposures of inhalatory doses of halothane, isoflurane, and the intravenous barbiturate thiamylal sodium in ponies to evoke a stronger response in the PIMs after four exposures at increasing intervals of 1, 2, and 6 weeks. Ultrastructural and cytochemical evidence is presented that halothane induced translocation of the surface coat into the vacuolar system of the PIMs, followed by expansion of the Golgi apparatus and its enrichment with acid phosphatase. The cell membrane was thrown into extraordinary lamellipodial extensions, which enabled the PIMs to interact with platelets within the narrow confines of the pulmonary capillaries. The relationship between PIMs and platelets developed into large platelet aggregates. Isoflurane and thiamylal sodium did not affect the circulating platelets, although the surface coat was translocated into the endolysosomes in both situations. Although isoflurane is a lipid-soluble inhalant anesthetic similar to halothane, it is subject to very little biotransformation after use and in the present model demonstrates no immune response.

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“…On the upper Louisiana slope of the Gulf of Mexico, the bivalve Bathymodiolus childressi is one of the most abundant and widespread species colonizing patchily distributed hydrocarbon seeps. B. childressi depends upon methanotrophic endosymbionts for the bulk of its nutrition (Childress et al 1986;Fisher and Childress 1992;Streams et al 1997) and can grow with methane as a sole carbon and energy source (Cary et al 1988). Nix et al (1995) found positive correlations between methane concentrations and B. childressi growth rates in situ and further suggested that toxic sulfide and hydrocarbon levels may negatively influence the growth and body condition of this mussel.…”

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confidence: 99%

Environment drives physiological variability in the cold seep mussel Bathymodiolus childressi

Bergquist

Fleckenstein

Szalai

et al. 2004

Limnology & Oceanography

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The ability of an organism to respond to changes in its environment depends upon its short-term physiological plasticity and the constraints of its genetic makeup. At hydrothermal vents and cold seeps, the spatially variable physiological characteristics of symbiont-bearing animals are often assumed to reflect short-term physiological adjustments to a patchy and dynamic chemical environment. However, the extent to which these spatially variable responses represent fixed characteristics unique to animals inhabiting the different environments (such as might arise from genetic differentiation) has not been tested. The seep mussel Bathymodiolus childressi depends upon methanotrophic bacteria for the bulk of its nutrition and inhabits a range of environments where it displays varying growth and body condition. In this study, we first investigated the multiscale environmental and physiological variability of B. childressi by measuring dissolved gas concentrations and mussel body condition in 12 mussel beds at four geographically distinct sites. Brine-dominated seeps tended to have higher methane and sulfide concentrations and host mussels of better body condition than petroleum-dominated sites. Then, using two transplant experiments, we evaluated whether local environmental conditions or stock effects determined the observed differences in growth and body condition of B. childressi. In all cases, mussels transplanted to new sites acquired or nearly acquired the characteristics of their host population, illustrating the primary role of the environment in determining the physiological characteristics of resident mussels. However, mussels from different sites sometimes responded differently to the same environment, suggesting stock-related effects also play a role in the spatial variation observed in the physiology of chemosynthetic fauna.

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Methanotrophic symbiont location and fate of carbon incorporated from methane in a hydrocarbon seep mussel

Cited by 71 publications

References 36 publications

Influence of CH<sub>4</sub> and H<sub>2</sub>S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel <i>Bathymodiolus azoricus</i>

Influence of CH<sub>4</sub> and H<sub>2</sub>S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel <i>Bathymodiolus azoricus</i>

In vivo interaction of pulmonary intravascular macrophages with activated platelets in microvessels of equine lung after multiple exposures to halothane, isoflurane, and thiamylal: A comparative ultrastructural and cytochemical study

Environment drives physiological variability in the cold seep mussel Bathymodiolus childressi

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Methanotrophic symbiont location and fate of carbon incorporated from methane in a hydrocarbon seep mussel

Cited by 71 publications

References 36 publications

Influence of CH&lt;sub&gt;4&lt;/sub&gt; and H&lt;sub&gt;2&lt;/sub&gt;S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel &lt;i&gt;Bathymodiolus azoricus&lt;/i&gt;

Influence of CH&lt;sub&gt;4&lt;/sub&gt; and H&lt;sub&gt;2&lt;/sub&gt;S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel &lt;i&gt;Bathymodiolus azoricus&lt;/i&gt;

In vivo interaction of pulmonary intravascular macrophages with activated platelets in microvessels of equine lung after multiple exposures to halothane, isoflurane, and thiamylal: A comparative ultrastructural and cytochemical study

Environment drives physiological variability in the cold seep mussel Bathymodiolus childressi

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Influence of CH<sub>4</sub> and H<sub>2</sub>S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel <i>Bathymodiolus azoricus</i>

Influence of CH<sub>4</sub> and H<sub>2</sub>S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel <i>Bathymodiolus azoricus</i>