Methanotrophic bacteria in oilsands tailings ponds of northern Alberta

Saidi‐Mehrabad, Alireza; He, Zhiguo; Tamaš, Ivica; Sharp, Christine E.; Brady, A. L.; Rochman, Fauziah F.; Bodrossy, Levente; Abell, Guy C. J.; Penner, Tara; Dong, Xiaoli; Sensen, Christoph Wilhelm; Dunfield, Peter F.

doi:10.1038/ismej.2012.163

Cited by 102 publications

(107 citation statements)

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“…8). We assumed (i) a cellular carbon content of 150 fg for exponentially growing bacterial cells (Vrede et al, 2002) and (ii) that about onethird of consumed CH 4 carbon is assimilated, with the remaining two-thirds respired to CO 2 (Bastviken et al, 2003;Roslev et al, 1997). C CH 4 ,assim exceeds MOB-C cell−growth by a factor of 9 to 17, indicating that some of the C CH 4,assim was available for secondary consumption by non-MOB.…”

Section: Structure Of the Methane Degrading Microbial Communitymentioning

confidence: 99%

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Methane-oxidizing seawater microbial communities from an Arctic shelf

et al. 2018

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Abstract. Marine microbial communities can consume dissolved methane before it can escape to the atmosphere and contribute to global warming. Seawater over the shallow Arctic shelf is characterized by excess methane compared to atmospheric equilibrium. This methane originates in sediment, permafrost, and hydrate. Particularly high concentrations are found beneath sea ice. We studied the structure and methane oxidation potential of the microbial communities from seawater collected close to Utqiagvik, Alaska, in April 2016. The in situ methane concentrations were 16.3 ± 7.2 nmol L −1 , approximately 4.8 times oversaturated relative to atmospheric equilibrium. The group of methaneoxidizing bacteria (MOB) in the natural seawater and incubated seawater was > 97 % dominated by Methylococcales (γ -Proteobacteria). Incubations of seawater under a range of methane concentrations led to loss of diversity in the bacterial community. The abundance of MOB was low with maximal fractions of 2.5 % at 200 times elevated methane concentration, while sequence reads of non-MOB methylotrophs were 4 times more abundant than MOB in most incubations. The abundances of MOB as well as non-MOB methylotroph sequences correlated tightly with the rate constant (k ox ) for methane oxidation, indicating that non-MOB methylotrophs might be coupled to MOB and involved in community methane oxidation. In sea ice, where methane concentrations of 82 ± 35.8 nmol kg −1 were found, Methylobacterium (α-Proteobacteria) was the dominant MOB with a relative abundance of 80 %. Total MOB abundances were very low in sea ice, with maximal fractions found at the icesnow interface (0.1 %), while non-MOB methylotrophs were present in abundances similar to natural seawater communities. The dissimilarities in MOB taxa, methane concentrations, and stable isotope ratios between the sea ice and water column point toward different methane dynamics in the two environments.

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Section: Structure Of the Methane Degrading Microbial Communitymentioning

confidence: 99%

“…These non-methane oxidizers are suggested to crossfeed on metabolites produced by the MOB (Hutchens et al, 2003;Jensen et al, 2008;Saidi-Mehrabad et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Methane-oxidizing seawater microbial communities from an Arctic shelf

et al. 2018

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“…Classification of OTUs is as given in Saidi-Mehrabad et al (2013). Bold and gray scripts denote MOB and methanol-oxidizer, respectively.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Biotic Interactions in Microbial Communities as Modulators of Biogeochemical Processes: Methanotrophy as a Model System

et al. 2016

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Microbial interaction is an integral component of microbial ecology studies, yet the role, extent, and relevance of microbial interaction in community functioning remains unclear, particularly in the context of global biogeochemical cycles. While many studies have shed light on the physico-chemical cues affecting specific processes, (micro)biotic controls and interactions potentially steering microbial communities leading to altered functioning are less known. Yet, recent accumulating evidence suggests that the concerted actions of a community can be significantly different from the combined effects of individual microorganisms, giving rise to emergent properties. Here, we exemplify the importance of microbial interaction for ecosystem processes by analysis of a reasonably well-understood microbial guild, namely, aerobic methane-oxidizing bacteria (MOB). We reviewed the literature which provided compelling evidence for the relevance of microbial interaction in modulating methane oxidation. Support for microbial associations within methane-fed communities is sought by a re-analysis of literature data derived from stable isotope probing studies of various complex environmental settings. Putative positive interactions between active MOB and other microbes were assessed by a correlation network-based analysis with datasets covering diverse environments where closely interacting members of a consortium can potentially alter the methane oxidation activity. Although, methanotrophy is used as a model system, the fundamentals of our postulations may be applicable to other microbial guilds mediating other biogeochemical processes.

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“…methanotrophs co-occur with other nonmethanotrophic bacteria and function collectively with them as a community to consume methane (18)(19)(20)(21)(22)(23)(24)(25). For instance, in the sediment of Lake Washington methane-utilizing communities are not random; they are dominated by methanotrophs within the family Methylococcaceae and nonmethanotrophic methylotrophs within the family Methylophilaceae and also include other specific non-methane-utilizing heterotrophs (18,19).…”

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confidence: 99%

Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions

Krause

Johnson

Karunaratne

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

152

144

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The utilization of methane, a potent greenhouse gas, is an important component of local and global carbon cycles that is characterized by tight linkages between methane-utilizing (methanotrophic) and nonmethanotrophic bacteria. It has been suggested that the methanotroph sustains these nonmethanotrophs by cross-feeding, because subsequent products of the methane oxidation pathway, such as methanol, represent alternative carbon sources. We established cocultures in a microcosm model system to determine the mechanism and substrate that underlay the observed cross-feeding in the environment. Lanthanum, a rare earth element, was applied because of its increasing importance in methylotrophy. We used co-occurring strains isolated from Lake Washington sediment that are involved in methane utilization: a methanotroph and two nonmethanotrophic methylotrophs. Gene-expression profiles and mutant analyses suggest that methanol is the dominant carbon and energy source the methanotroph provides to support growth of the nonmethanotrophs. However, in the presence of the nonmethanotroph, gene expression of the dominant methanol dehydrogenase (MDH) shifts from the lanthanide-dependent MDH (XoxF)-type, to the calcium-dependent MDH (MxaF)-type. Correspondingly, methanol is released into the medium only when the methanotroph expresses the MxaF-type MDH. These results suggest a cross-feeding mechanism in which the nonmethanotrophic partner induces a change in expression of methanotroph MDHs, resulting in release of methanol for its growth. This partner-induced change in gene expression that benefits the partner is a paradigm for microbial interactions that cannot be observed in studies of pure cultures, underscoring the importance of synthetic microbial community approaches to understand environmental microbiomes. M icrobial communities and their members are part of every ecosystem and drive important biogeochemical processes on Earth (1). They typically comprise a range of phylogenetically and functionally diverse microbes (2) that are structured by biotic and abiotic factors (3) and are entangled through specific interactions in complex networks (4).The significance of microbial communities in diverse ecosystems including the human body is now widely accepted in science and has led to a range of initiatives focused on the world's microbiomes (5, 6). One important goal within these efforts is to understand how microbes interact with each other and the consequences of such interactions at the level of their transcriptomes, proteomes, and metabolomes. For instance, it has been demonstrated that the metabolism of yeast can be transformed by bacteria-induced prions to decrease the release of inhibiting ethanol (7). In another study, an oral biofilm of the genus Streptococcus displayed different transcriptional responses toward the presence of other species in mixed-species cultures (8).Measuring interactions in complex environmental communities is still a difficult task. Laboratory cocultures represent a simplified approach to assessing...

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Methanotrophic bacteria in oilsands tailings ponds of northern Alberta

Cited by 102 publications

References 70 publications

Methane-oxidizing seawater microbial communities from an Arctic shelf

Methane-oxidizing seawater microbial communities from an Arctic shelf

Biotic Interactions in Microbial Communities as Modulators of Biogeochemical Processes: Methanotrophy as a Model System

Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions

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