Methanotroph (Methylococcus capsulatus, Bath) bacteria meal as an alternative protein source for Japanese yellowtail, Seriola quinqueradiata

Biswas, Amal; Takakuwa, Fumiaki; Yamada, Shinichi; Matsuda, Akihisa; Saville, Renée M.; LeBlanc, Allan; Silverman, Julian; Sato, Nobumitsu; Tanaka, Hideki

doi:10.1016/j.aquaculture.2020.735700

Cited by 50 publications

(50 citation statements)

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“…In agreement with Chen et al (2021), this study has shown the substitution of fishmeal with methanotroph bacteria meal did not affect uptake of feed nor have any other detrimental effects such as antinutritional properties or impacts on a range of immune measures. These results are in broad agreement with studies showing dietary supplementation of fishmeal with single cell protein shows no significant differences in growth and feed efficiency in fish species such as Atlantic salmon (Berge et al, 2005), Atlantic halibut (Aas et al, 2007), and Japanese yellowtail (Biswas et al, 2020). Other research on single cell protein as replacement of fishmeal in shrimp diets has shown they can be used to replace fishmeal, partially or fully, e.g., purple non-sulfur bacteria (Chumpol et al, 2018), C. ammoniagenes (Hamidoghli et al, 2019), and KnipBio Meal (Tlusty et al, 2017).…”

Section: Discussionsupporting

confidence: 91%

“…The harvested biomass is centrifuged, heat inactivated, and spray dried. The nutrient content and amino acid profile of methanotroph bacteria meal, FeedKind R , is comparable to traditional proteins used in animal feeds such as fishmeal (Biswas et al, 2020) and has been shown to be a viable protein source for popular species of aquaculture fish including rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar) and yellowtail (Seriola quinqueradiata) (Berge et al, 2005;Øverland et al, 2006;Biswas et al, 2020). An additional benefit of producing methanotroph bacteria meal, is that it utilizes less than 0.01% of the land and around 10% of blue water compared with that used to produce soy protein, enhancing its sustainability credentials.…”

Section: Introductionmentioning

confidence: 97%

“…Methanotrophs are gram-negative bacteria that use methane as their sole source of carbon and energy and have been identified for their potential use as a protein source in feeds for a variety aquaculture species (Berge et al, 2005;Øverland et al, 2006;Aas et al, 2007;Øverland et al, 2011;Biswas et al, 2020;Chen et al, 2021). Whilst studies have investigated the use of bacterial derived single cell protein feed additives from Corynebacterium ammoniagenes (Hamidoghli et al, 2019) and purple non-sulfur bacteria Rhodobacter sphaeroides and Afifella marina (Chumpol et al, 2018) in shrimp diets, to date only one published study has evaluated the use of Methylococcus capsulatus as an alternative protein source for shrimp feed formulations with up to 45% of the fish meal replaced with FeedKind R followed by exposure to Vibrio parahaemolyticus by injection (Chen et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Effects of a Single Cell Protein (Methylococcus capsulatus, Bath) in Pacific White Shrimp (Penaeus vannamei) Diet on Growth Performance, Survival Rate and Resistance to Vibrio parahaemolyticus, the Causative Agent of Acute Hepatopancreatic Necrosis Disease

et al. 2021

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The efficacy of a single cell protein (SCP) methanotroph (Methylococcus capsulatus, Bath) bacteria meal (FeedKind®, Calysta, Menlo Park, CA, United States), in Pacific white shrimp (Penaeus vannamei) diets was studied to determine growth performance, survival rate and disease resistance against Vibrio parahaemolyticus causing Acute Hepatopancreatic Necrosis Disease (AHPND). The growth trial was assigned in a completely randomized design (CRD) with four treatments and 5 replicates of each, T1: a fishmeal-based control containing 15% fish meal and 3 diets with graded levels of methanotroph bacteria meal, namely T2: 5% methanotroph bacteria meal, T3: 10% methanotroph bacteria meal, and T4: 15% methanotroph bacteria meal. Shrimp were fed ad libitum for 6 weeks on trial diets to assess growth. Subsequent to the growth trial, three replicates of the same groups were exposed to V. parahaemolyticus by a single bath challenge and held for a further 15 days on the same diets as the growth study to assess survival and resistance. No significant differences (p > 0.05) in survival or in growth performance, including final weight, weight gain, specific growth rate, feed consumption or feed conversion ratio of white shrimp fed feeds containing methanotroph bacteria meal or control diets for 6 weeks. Immune markers such as hemocyte counts, phenoloxidase, superoxide dismutase and lysozyme activity were similar across all groups after the 6-week feeding trial. In a V. parahaemolyticus challenge, methanotroph bacteria meal in the diet significantly promoted the survival rate, and the reduction of Vibrio sp. in the hepatopancreas of white shrimp. Hemocyte count and phenoloxidase activity showed no significant differences (p > 0.05) between diet treatment groups, but hemolymph protein was significantly higher (p < 0.05) in shrimp fed diets containing 15% methanotroph bacteria meal after challenge. The Vibrio colony counts from hepatopancreas in the treatment groups were all significantly lower than the control (p < 0.05). The findings show that methanotroph bacteria meal can entirely replace fishmeal in white shrimp diets and the 15% inclusion of methanotroph bacteria meal in shrimp diet shows no adverse effects on growth performance, feed utilization and survival rate. In addition, shrimp fed methanotroph bacteria meal diets exhibited improved survival rates to an AHPND challenge.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Effects of a Single Cell Protein (Methylococcus capsulatus, Bath) in Pacific White Shrimp (Penaeus vannamei) Diet on Growth Performance, Survival Rate and Resistance to Vibrio parahaemolyticus, the Causative Agent of Acute Hepatopancreatic Necrosis Disease

et al. 2021

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“…Use of methanotrophs in the industry today is limited to the generation of biomass for animal feed from single cell protein (Biswas et al 2020 ; Tsapekos et al 2020 ; Øverland et al 2010 ). Companies like Calysta and Unibio have successfully industrialized and commercialized gaseous fermentation by methanotrophs for the generation of feed biomass using a U-loop fermentor (Larsen 2002 ).…”

Section: Applications Of Native Methanotrophs and Methane Monooxygenamentioning

confidence: 99%

Methane monooxygenases: central enzymes in methanotrophy with promising biotechnological applications

Khider

Brautaset

Irla

2021

World J Microbiol Biotechnol

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Worldwide, the use of methane is limited to generating power, electricity, heating, and for production of chemicals. We believe this valuable gas can be employed more widely. Here we review the possibility of using methane as a feedstock for biotechnological processes based on the application of synthetic methanotrophs. Methane monooxygenase (MMO) enables aerobic methanotrophs to utilize methane as a sole carbon and energy source, in contrast to industrial microorganisms that grow on carbon sources, such as sugar cane, which directly compete with the food market. However, naturally occurring methanotrophs have proven to be difficult to manipulate genetically and their current industrial use is limited to generating animal feed biomass. Shifting the focus from genetic engineering of methanotrophs, towards introducing metabolic pathways for methane utilization in familiar industrial microorganisms, may lead to construction of efficient and economically feasible microbial cell factories. The applications of a technology for MMO production are not limited to methane-based industrial synthesis of fuels and value-added products, but are also of interest in bioremediation where mitigating anthropogenic pollution is an increasingly relevant issue. Published research on successful functional expression of MMO does not exist, but several attempts provide promising future perspectives and a few recent patents indicate that there is an ongoing research in this field. Combining the knowledge on genetics and metabolism of methanotrophy with tools for functional heterologous expression of MMO-encoding genes in non-methanotrophic bacterial species, is a key step for construction of synthetic methanotrophs that holds a great biotechnological potential.

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“…In recent years, however, bacterial protein meal (BPM) with high protein content (approximately 70%) has been produced from the bacterium Methylococcus capsulatus (Bath), which utilizes methane as a carbon and energy source. Reportedly, BPM has the potential to serve as an alternative protein source for different species, although its use has yielded some mixed results (Aas et al, , 2007Aas, Hatlen, et al, 2006;Berge et al, 2005;Biswas et al, 2020;Øverland et al, 2006Perera et al, 1995;Storebakken et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Partial replacement of fishmeal by a protein‐rich product from methanotrophic bacteria in juvenile red sea bream ( Pagrus major )

et al. 2021

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Methanotroph (Methylococcus capsulatus, Bath) bacteria meal as an alternative protein source for Japanese yellowtail, Seriola quinqueradiata

Cited by 50 publications

References 41 publications

Effects of a Single Cell Protein (Methylococcus capsulatus, Bath) in Pacific White Shrimp (Penaeus vannamei) Diet on Growth Performance, Survival Rate and Resistance to Vibrio parahaemolyticus, the Causative Agent of Acute Hepatopancreatic Necrosis Disease

Effects of a Single Cell Protein (Methylococcus capsulatus, Bath) in Pacific White Shrimp (Penaeus vannamei) Diet on Growth Performance, Survival Rate and Resistance to Vibrio parahaemolyticus, the Causative Agent of Acute Hepatopancreatic Necrosis Disease

Methane monooxygenases: central enzymes in methanotrophy with promising biotechnological applications

Partial replacement of fishmeal by a protein‐rich product from methanotrophic bacteria in juvenile red sea bream ( Pagrus major )

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