Methanolic leaf exctract of Otostegia integrifolia Benth reduces blood glucose levels in diabetic, glucose loaded and normal rodents

Abstract

BackgroundAlthough the leaves of Otostegia integrifolia traditionally claimed in Ethiopian folklore medicine for management of diabetes mellitus, it has not been validated. The aim of this study was therefore to investigate the antidiabetic activity of Otostegia integrifolia in rodents.MethodsMale rats or mice were randomly divided into five groups for diabetic, hypoglycemic and glucose tolerance test (OGTT) studies. In all models, group I received Tween 80, 2% v/v, Group II (GL5) treated with 5 mg/kg of glibenclamide and the remaining group: III, IV and V were given the plant extract at doses of 100 mg/kg 200 mg/kg and 400 mg/kg respectively. Blood glucose levels (BGL) were measured by collecting blood samples at different time points. Data was analyzed using one way ANOVA followed by Dunnet’s post hoc test to carry out between and within group comparisons. P < 0.05 was considered as statistically significant.ResultsInter-group analysis revealed that O. integrifolia at 100 mg/kg and 200 mg/kg reduced the 4th hour fasting blood BGL significantly (p < 0.001) compared to the control group. The intra-group analysis result has shown O. integrifolia at 200 mg/kg produced a significant (p < 0.05) reduction in BGL at the 1st, 2nd, 3rd and 4th hours of post treatment compared to their respective initial levels. Moreover, in the hypoglycemic and OGTT models, O. integrifolia extract at 200 mg/kg, has shown a significant reduction in blood glucose levels compared to negative controls and across all time points.ConclusionThe crude extract of O. integrifolia has shown significant antidiabetic, hypoglyceamic and oral glucose tolerating effects. The effective dose of the extract was found to be 200 mg/kg in time dependent manner.

Review of yoga therapy during cancer treatment

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“…Participants included a mean of 10 children with heterogeneous cancer diagnoses. All studies included parents/caregivers as yoga participants [32-34] and/or proxy respondents [32-33, 35]. …”

“…Dose varied from a single 45-minute session to 8-24, 60-minute sessions, with 1 to 3 sessions per week, spread over 3-12 weeks. Three interventions were implemented as group classes [33-35]; one study did not specify delivery format [32]. None reported whether they provided instructions for home practice.…”

Antidiabetic effects of Salvia triloba and Thymus praecox subsp. skorpilii var. skorpilii in a rat model of streptozotocin/nicotinamide-induced diabetes

“…Recent studies has estimated that approximately 30% of patients with DM take complementary and alternative medicine [10]. Medicinal plants and its products proceed to be an important therapeutic alternative for relieving the ailments.…”

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