Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells

Abstract

Carcinoma of the uterine cervix is the second most common female tumor worldwide, surpassed only by breast cancer. Natural products from seaweeds evidencing apoptotic activity have attracted a great deal of attention as new leads for alternative and complementary preventive or therapeutic anticancer agents. Here, methanol extracts from 13 species of tropical seaweeds (Rhodophytas, Phaeophyta and Chlorophyta) collected from the Northeast of Brazil were assessed as apoptosis-inducing agents on human cervical adenocarcinoma (HeLa). All extracts showed different levels of cytotoxicity against HeLa cells; the most potent were obtained from the brown alga Dictyota cilliolata (MEDC) and Dictyota menstrualis (MEDM). In addition, MEDC and MEDM also inhibits SiHa (cervix carcinoma) cell proliferation. Studies with these two extracts using flow cytometry and fluorescence microscopy showed that HeLa cells exposed to MEDM and MEDC exhibit morphological and biochemical changes that characterize apoptosis as shown by loss of cell viability, chromatin condensation, phosphatidylserine externalization, and sub-G1 cell cycle phase accumulation, also MEDC induces cell cycle arrest in cell cycle phase S. Moreover, the activation of caspases 3 and 9 by these extracts suggests a mitochondria-dependent apoptosis route. However, other routes cannot be ruled out. Together, these results point out the methanol extracts of the brown algae D. mentrualis and D. cilliolata as potential sources of molecules with antitumor activity.

Effect of dietary inclusion of fermented sea mustard by‐product on growth performance, blood profiles, and meat quality in broilers

“…Approximately 1 g excreta sample from each cage was blended with 9 mL of 1% peptone broth (Becton, Dickinson and Co., Franklin Lakes, NJ) and then homogenized. The viable counts of Lactobacillus and E. coli were conducted by plating serial ten‐fold dilutions (10−1 to 10−8) onto lactobacilli medium III agar plates (Medium 638; DSMZ, Braunschweig, Germany) and MacConkey agar plates (Difco Laboratories, Detroit, MI, USA) to isolate Lactobacillus and E. coli , respectively. The lactobacilli medium III agar plates were then incubated for 48 h at 37 °C under anaerobic conditions.…”

“…As people become more aware of the relationship between diet and health, the consumption of seaweed (marine macroalgae) products are increasingly gaining attention. It is a rich nutrient source of food which mainly contains proteins, amino acids, non‐protein nitrogen, lipids, dietary fiber, vitamins and minerals, and is a source of various bioactive components in biomedical pharmaceutical and health products which have been applied in anti‐inflammatory, antiviral, anticoagulant, antitumoral activities, antiobesity, antidiabetic, antihypertensive and antihyperlipidemic . One of the most popular seaweeds is sea mustard, also known as Undaria pinnatifida (wakame).…”

Bothrops jararacaandBothrops erythromelasSnake Venoms Promote Cell Cycle Arrest and Induce Apoptosis via the Mitochondrial Depolarization of Cervical Cancer Cells

“…10.000 events were acquired. The FlowJo software version X10.0.7 (Tree Star, Inc., Ashland, OR, USA) was used for data analysis as described in Gomes et al, 2015 [26]. …”

In VivoEvaluation of the Antioxidant Activity and Protective Action of the SeaweedGracilaria birdiae

“…Phenolic compounds were quantified by the colorimetric method with the Folin-Ciocalteu reagent using gallic acid as a standard [ 21 ].…”