Methanolic extract of Potentilla fulgens root and its ethyl-acetate fraction delays the process of carcinogenesis in mice

Abstract

People of north-eastern states of India consume raw areca-nut (RAN) and lime which could lead to oral, esophageal and gastric cancers. However, the incidence of these cancers are significantly lesser in those who consume pieces of Potentilla fulgens root along with RAN. Since evaluation of anticancer role, if any, of P. fulgens on RAN-mediated genetic alterations in human is difficult because of other compounding factors, this study was undertaken in mice to focus on gastric carcinogenesis since ad libitum administration of RAN extract with lime in drinking water induced stomach cancer due to greater exposure of its lining. A total of 160 mice were used at different time points and either methanol extract of P. fulgens roots (PRE) or mixture of four compounds of ethyl-acetate fraction (EA-mixture) was mixed with mice feed. Histological studies revealed that RAN + lime induced cancer in all the mice and interestingly only 20% developed cancer when PRE/EA-mixture was provided along with RAN + lime. Higher frequency of precocious anaphase and over expression of p53 and Securin genes were significantly reduced by PRE/EA-mixture. Thus PRE/EA-mixture mitigates the RAN-induced tumor-initiating process in stomach by maintaining expression of tumor suppressor and check-point genes under control.

High‐performance thin layer chromatography method development, validation, and assessment of enzymatic inhibition potential of Potentilla fulgens hydroalcoholic roots extract

“…Catechin (C 15 H 14 O 6 ; Molecular mass: 290.26) (Figure 1) is a polyphenol compound available in several plants including Potentilla species which has antioxidant activity and is used as a memory enhancer and for lowering the cholesterol in the blood [19][20][21]. In the study conducted by Ganguly et al [22] and Choudhary et al [17] the catechin showed potent anticancer and antioxidant activity respectively.…”

The complete chloroplast genome and phylogenetic analysis of Potentilla sischanensis Bunge ex Lehm

“…Extracts from some species can also delay the process of carcinogenesis (Ganguly et al. 2019 ; Kowalik et al. 2020 ).…”