Methanolic extract of Condalia buxifolia added to transport water alters biochemical parameters of the silver catfish Rhamdia quelen

Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

“…Protein carbonyl content is actually the most commonly used marker of protein oxidation (Almroth et al, 2005;Salbego et al, 2014Salbego et al, , 2015. In this study, silver catfish transported with EUG and EOL showed higher PC content than control fish, indicating higher protein oxidation.…”

“…The results of the present study suggest that the addition of EUG and EOL may reduce oxygen consumption, ultimately improving the physiological condition of fish during transport. Stress negatively affects protein metabolism, inhibiting the synthesis and stimulating the catabolism of proteins (Mommsen et al, 1999;Becker et al, 2016;Salbego et al, 2015;Toni et al, 2015). The addition of EUG and EOL to the water during silver catfish transport likely reduced protein catabolism, since protein levels were higher in the muscle and liver than in control group at the end of transport.…”

“…The attenuation of oxidative damage may also be favored by the efficiency of the antioxidant defense system (Goyal et al, 2013). Increases in NPSH levels attenuate oxidative stress (Ungvari et al, 2009;Yonar, 2012;Salbego et al, 2014Salbego et al, , 2015 and the higher NPSH content in the liver of fish after transport in EUG-and EOL-containing water may have prevented lipid damage. On the other hand, GST activity was lower in the liver of silver catfish transported with EUG, while the addition of EOL did not affect the activity of this enzyme.…”

“…The metabolic and antioxidant responses of fish exposed to anesthetics have been scarcely investigated (Azambuja et al, 2011;Becker et al, 2012Becker et al, , 2016Salbego et al, 2014Salbego et al, , 2015Toni et al, 2015). Transportation in closed systems leads to changes in the metabolic state of fish and may lead to oxidative damage through the production of reactive oxygen species (ROS).…”

Essential oils of Cunila galioides and Origanum majorana as anesthetics for Rhamdia quelen: efficacy and effects on ventilation and ionoregulation

“…This species tolerates well the cold winter of the region and presents a high reproductive rate and a rapid weight gain during the warmer months, even when raised in artificial tanks or in polyculture together with other fish species (Gomes et al, 2000;Baldisserotto, 2009 Boost in aquaculture production and economic importance of R. quelen should run parallel with technological improvements in order to preserve life quality of the farmed fish. Among the many factors which may interfere negatively in the animal's living conditions and their performance are procedures such as capture, blood collection, tank transfer, confinement and transport, which are associated with stress (Barton et al, 1980;Salbego et al, 2015). In this context, the use of anesthetics as MS-222 and metomidate during fish handling represents an alternative to minimize such inconveniences.…”