Methanolic extract of Citrullus colocynthis suppresses growth and proliferation of breast cancer cells through regulation of cell cycle

Perveen, Sadia; Ashfaq, Hanfa; Ambreen, Saira; Ashfaq, Isbah; Kanwal, Zakia; Tayyeb, Asima

doi:10.1016/j.sjbs.2020.11.029

Cited by 19 publications

(12 citation statements)

References 23 publications

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“…Bioassays showed a marked reduction in the multiplication and growth of cells treated with these extracts compared to untreated cells. The presence of cyclin-CDK inhibitors means that C. colocynthis extract can arrest human breast cancer cells ( Perveen et al, 2021 ). Colocynth fruit pulp extracts can also block the proliferation and metastatic activity of breast cancer cells and prevent cell migration, the induction of cell apoptosis and cell proliferation, and inhibit cancer stemness properties in breast cancer cells ( Chowdury et al, 2017 ).…”

Section: Pharmacological Effects Of C Colocynthismentioning

confidence: 99%

“…Bioassays showed a significant reduction in the multiplication and growth of treated cells compared to untreated cells. Owing to the expression of cyclin-CDK inhibitors, C. colocynthis arrests the cell cycle in human breast tumor cells ( Perveen et al, 2021 ). C. colocynthis is used for treating colorectal cancer in humans.…”

Section: Applications In Humansmentioning

confidence: 99%

Section: Anti-tumor Activitymentioning

confidence: 99%

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Citrullus colocynthis (L.) Schrad (Bitter Apple Fruit): Promising Traditional Uses, Pharmacological Effects, Aspects, and Potential Applications

Munawar

Saeed

et al. 2022

Front. Pharmacol.

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Health consciousness and increased knowledge about the side effects of synthetic drugs have enhanced interest in traditional medicines. Medicinal plants offer cures for various diseases, leading to improved living standards. This has brought ethnomedicinal studies into the spotlight and increased demand for herb-based medicines. Citrullus colocynthis is an herbaceous plant containing an abundance of nutrients that play a key role in the improvement of wellbeing. C. colocynthis has many biological properties, such as antioxidative, hypoglycemic, antibacterial, anti-cancerous, anti-inflammatory, analgesic, gastrointestinal tract, reproduction, protection, anti-microbial, antidiabetic, hypolipidemic, antineoplastic, profibrinolytic, anti-allergic, pesticidal, and immune-stimulatory. There are numerous bioactive compounds like cucurbitacin, flavonoids, and polyphenols in C. colocynthis that give it medicinal properties. Herein, we have extensively compiled, reviewed, and analyzed significant information on C. colocynthhis from the best published available evidence in PubMed, Scopus (Embase), Web of Science (Web of Knowledge), Cochrane Library, and Google Scholar, etc. Scientific literature evidenced that owing to the bioactive constituents, including cucurbitacin, polyphenols, flavonoids, and other potent molecules, C. colocynthis has many pharmacological and physiological functions. It possesses multi-beneficial applications in treating various disorders of humans and animals. So, the primary purpose of this comprehensive review is to provide an overview of the findings of positive impacts and risks of C. colocynthis consumption on human health, especially in poultry and veterinary fields. In the future, this narrative article will be aware of discoveries about the potential of this promising natural fruit and its bioactive compounds as the best nutraceuticals and therapeutic drugs in veterinary and human medicine.

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Section: Pharmacological Effects Of C Colocynthismentioning

confidence: 99%

Section: Applications In Humansmentioning

confidence: 99%

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Citrullus colocynthis (L.) Schrad (Bitter Apple Fruit): Promising Traditional Uses, Pharmacological Effects, Aspects, and Potential Applications

Munawar

Saeed

et al. 2022

Front. Pharmacol.

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“…This plant is native to the Mediterranean region and Asia, however, it can be found in deserts throughout Asia, Europe, and North Africa. The fruits are utilized in Indian traditional medicine to treat a variety of ailments, and people use them to treat joint inflammation, rheumatism, and joint pain 12 . According to a literature survey, C. colocynthis also has anti-inflammatory, antioxidant, anti-diabetic, antibacterial and anti-cancer properties 13 .…”

Section: Introductionmentioning

confidence: 99%

Comparative Antioxidant and Anti-gout Activities of Citrullus colocynthis loaded Fruit Silver nanoparticles with its Ethanolic extract

Karunakaran¹,

Hari²

2022

AJMB

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Background: The biological synthesis of silver nanoparticles (AgNPs) using plant materials is a rapidly developing method with several alternative medical applications. This comparative study of ethanolic fruit extract of Citrullus colocynthis (C. colocynthis) (EFECC) and synthesized silver nanoparticles (CC-AgNPs) were carried out for antioxidants and anti-gout arthritic activities. Methods: The AgNPs were synthesized using C. colocynthis fruit and its characterization was done by UV-visible spectroscopy, TEM, XRD and FT-IR. The 90% ethanol was used for extract preparation. Antioxidant activity was analyzed by DPPH and the Hydrogen Peroxide (H2O2) method. In vitro anti-arthritic activity was tested by xanthine oxidase inhibition, protein denaturation and HRBC membrane stabilization assay. Results: The synthesized CC-AgNPs were confirmed by UV-vis spectroscopy and TEM images displayed spherical shapes with 10-45 nm size range. Furthermore, the functional groups and crystalline structure of CC-AgNPs were determined by FT-IR and XRD analysis. The biosynthesized CC-AgNPs exhibited an excellent free radical scavenging ability than EFECC. In anti-arthritic activity, the CC-AgNPs showed effective inhibition of xanthine oxidase production, protein denaturation, and damaged RBC membranes compared to EFECC. Conclusion: The antioxidant activities and in vitro anti-arthritic assays revealed that CC-AgNPs are better anti-gout agents than EFECC. This research suggested that biosynthesized silver nanoparticles from C. colocynthis fruit are an important target in the field of anti-gout drug discovery.

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“…Throughout history, humans have used the fruits, seeds, leaves, and roots of C. colocynthis to prevent and treat various illnesses, including dermatological conditions as leprosy, digestive disorders as constipation and jaundice, respiratory diseases as asthma and bronchitis, and many other ailments such as rheumatism, hypertension, mastitis, and pain [4][5][6][7]. Other biomedical applications of C. colocynthis include anti-cancer [8][9][10][11][12][13][14][15], anti-diabetic [16][17][18], anti-lipidemic [19][20][21][22], antimicrobial [23,24] and insecticidal [25,26]. Most recent investigations have further hinted at its preventive and healing effects on various complications of diabetes, including diabetic nephropathy [27][28][29], glycated hemoglobin [30], and deteriorated cognitive performance [31].…”

Section: Introductionmentioning

confidence: 99%

Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

et al. 2021

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Background The medicinal plant Citrullus colocynthis (L.) Schrad. (C. colocynthis) may benefit patients at different phases of diabetes by attuning to contrasting situations. Our primary objective was to find the mechanism(s) behind the antidiabetic/anti-hyperlipidemic effects of C.colocynthis seed aqueous extract (CCAE) in two different stages of type 2 diabetes (T2D) in rats. Methods Fasting blood sugar (FBS) levels, body weights, and the degree of impaired glucose tolerance (IGT) were measured in healthy nondiabetic control rats (Con), as well as rats with early and late stages of T2D, denoted as ET2D and LT2D, respectively. CCAE was intraperitoneally (IP) injected for 28 days. In the end, the hepatic mRNA expression levels of the following genes were determined by RT-PCR: glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), insulin-dependent sterol regulatory element-binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), peroxisome proliferator-activated receptor alpha (PPARα), and carnitine palmitoyltransferase I (CPT1). The liver was examined by hematoxylin and eosin (H&E) and Oil-Red O staining. CCAE was partially analyzed by HPLC-DAD. Results ET2D and LT2D were characterized by differentially elevated FBS, deteriorated bodyweight, and significant IGT compared to Con. Hepatosteatoses of varying morphologies and higher hepatic expression of G6Pase than PRPCK in ET2D versus the opposite in LT2D further confirmed the divergent nature of metabolic aberrations. At the end of 28 days, the high levels of FBS, alkaline phosphatase (ALP), triglyceride (TG), urea, hepatic protein carbonyl content (PCC), and alanine and aspartate aminotransferases (AST and ALT, respectively) persisted in untreated LT2D. CCAE ameliorated oxidative stress and upregulated PPARα expression in diabetic groups and Con; it downregulated CPT1 expression in the LT2D group. CCAE’s ability to lower FBS and serum and hepatic TG in both ET2D and LT2D indicated its ability to act via different mechanisms. Ferulic acid (Fer A) and rutin hydrate (RH) were detected in CCAE. Conclusion CCAE lowered the FBS in ET2D via inhibiting the hepatic G6Pase expression (glycogenolysis). In LT2D, CCAE abated sugar levels by diverting PEPCK activity, preferably towards glyceroneogenesis than gluconeogenesis. The preserved triglyceride/fatty acid (TG/FA) cycle, the upregulated PPARα, and the downregulated CPT1 gene expressions reduced serum and hepatic TG.

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Methanolic extract of Citrullus colocynthis suppresses growth and proliferation of breast cancer cells through regulation of cell cycle

Cited by 19 publications

References 23 publications

Citrullus colocynthis (L.) Schrad (Bitter Apple Fruit): Promising Traditional Uses, Pharmacological Effects, Aspects, and Potential Applications

Citrullus colocynthis (L.) Schrad (Bitter Apple Fruit): Promising Traditional Uses, Pharmacological Effects, Aspects, and Potential Applications

Comparative Antioxidant and Anti-gout Activities of Citrullus colocynthis loaded Fruit Silver nanoparticles with its Ethanolic extract

Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

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