Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G<sub>0</sub>/G<sub>1</sub> and upregulating Bax

Wan-Ibrahim, Wan Suriyani; Ismail, Norzila; Mohd-Salleh, Siti Farhanah; Yajid, Aidy Irman; Wong, Michael Pak-Kai; Hashim, Mohd Nizam Md

doi:10.4103/2221-1691.260397

Cited by 6 publications

(1 citation statement)

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“…Notably, it has been documented that the methanolic extracts of Berberis hispanica, Solanum schimperianum, and Abrus precatorius inhibited MDA-MB-231 cell proliferation by 50% after 24 to 72hrs of incubation at a dose of 16.5 ± 0.6µg/ml, 7 ± 0.25µg/ml, and 26.4 ± 5.46µg/ml, respectively. [20][21][22] Apoptosis (programmed cell death) is a process in which cells self-destruct when triggered by the appropriate stimuli. Typically, the cells are maintained and regulated by a constant balance between cell division and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Illicium verum anticancer activity against MDA-MB-231 cell line

Pahore,

Khan,

Karim

2023

Pak J Med Sci

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Objective: To evaluate the anticancer activity of methanolic extract of Illicium verum against triple-negative breast cancer MDA-MB-231 cell line. Methods: A cell culture experimental study was carried out at Pharmacology department of Bahria University Medical and Dental College (January to June 2021) in collaboration with Aga Khan University, Karachi, Pakistan. Cell viability and proliferation assays were used to quantify dead and alive cells by utilizing a tetrazolium assay and an enzyme immunosorbent plate reader was used to calculate their absorbance. For the apoptosis initiation assay, these cells were dyed with a fluorescent stain and observed for fluorescence and apoptosis. During cell viability testing, various I. verum methanolic extract doses (0.125, 0.25, 0.5, 1, 3, 6, 12, and 25µg/ml) were employed to treat MDA-MB-231 cells, while the IC50 dose of 2.8µg/ml was used for both the cell proliferation and apoptosis initiation assays. Results: In the cell viability assay, all I. verum methanolic extract doses exhibited a substantial decrease in the viability of MDA-MB-231 cells (less than 0.01 p-value). In cell proliferation assay and apoptosis initiation, the IC50 dose of 2.8µg/ml of I. verum methanolic extract also exhibited a substantial decrease in cell division (less than 0.01 p-value) and the initiation of apoptosis in MDA-MB-231 cells. Conclusion: Illicium verum methanolic extract have strong anticancer activity against triple-negative breast cancer MDA-MB-231 cell line through cytotoxicity, proliferation reduction, and apoptosis initiation mechanisms. doi: https://doi.org/10.12669/pjms.40.1.7860 How to cite this: Pahore AK, Khan S, Karim N. Illicium verum anticancer activity against MDA-MB-231 cell line. Pak J Med Sci. 2024;40(1):---------. doi: https://doi.org/10.12669/pjms.40.1.7860 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%