Methanol utilization in defined mixed cultures of thermophilic anaerobes in the presence of sulfate

Goorissen, Heleen P.; Stams, A.J.M.; Hansen, Theo A.

doi:10.1016/j.femsec.2004.05.004

Cited by 20 publications

(17 citation statements)

References 21 publications

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“…The presence of two methanol-degradation pathways may be beneficial for D. kuznetsovii in its deep-subsurface habitat where it has to compete with other methylotrophic anaerobes. Generally, methanogens and acetogens grow faster with methanol than sulfate reducers, but their growth is hampered by cobalt limitation 35 , 36 , 45 . Methanogens appear to compete better for cobalt during cobalt-limiting conditions 36 , while acetogens outcompete methanogens when the concentrations of methanol and cobalt are high 35 .…”

Section: Resultsmentioning

confidence: 99%

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

et al. 2018

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Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.

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Section: Resultsmentioning

confidence: 99%

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

et al. 2018

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“…Butyrate fermentation to acetate, for example, yields only 2H 2 (or 4 eq of H 2 ) per mole of butyrate; therefore, expressed on the basis of expected hydrogen produced from primary fermentation of butyrate, our dose rate of 0.6 mmol/L would have produced only 2.4 meq/L of H 2 -far less than the 12 meq/L of hydrogen added directly to the H 2 -reactor and insufficient, even, to handle the 4 meq/L of PCE added. A similar estimate of H 2 from methanol is not possible since methanol conversion does not necessarily result in a net H 2 production; conversion of methanol to H 2 /CO 2 is only energetically favorable at low hydrogen concentrations and therefore depends on the relative activity of methanol-fermenters and H 2 -users (e.g., dechlorinators, methanogens, sulfate reducers) (Balk et al, 2002;Cord-Ruwish and Ollivier, 1986;Goorissen et al, 2004). In the absence of fast and efficient H 2 -consumption methanol-fermenters will mostly produce acetate instead of H 2 /CO 2 .…”

Section: Discussionmentioning

confidence: 99%

Comparative study of methanol, butyrate, and hydrogen as electron donors for long‐term dechlorination of tetrachloroethene in mixed anerobic cultures

Aulenta

Gossett

Papini

et al. 2005

Biotech & Bioengineering

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This study examined the ability of different electron donors (i.e., hydrogen, methanol, butyrate, and yeast extract) to sustain long-term (500 days) reductive dechlorination of tetrachloroethene (PCE) in anerobic fill-and-draw bioreactors operated at 3:1 donor:PCE ratio (defined on a total-oxidation basis for the donor). Initially (i.e., until approximately day 80), the H(2)-fed bioreactor showed the best ability to completely dechlorinate the dosed PCE (0.5 mmol/L) to ethene whereas, in the presence of methanol, butyric acid or no electron donor added (but low-level yeast extract), dechlorination was limited by the fermentation of the organic substrates and in turn by H(2) availability. As the study progressed, the H(2)-fed reactor experienced a diminishing ability to dechlorinate, while more stable dechlorinating activity was maintained in the reactors that were fed organic donors. The initial diminished ability of the H(2)-fed reactor to dechlorinate (after about 100 days), could be partially explained in terms of increased competition for H(2) between dechlorinators and methanogens, whereas other factors such as growth-factor limitation and/or accumulation of toxic and/or inhibitory metabolites were shown to play a role for longer incubation periods (over 500 days). In spite of decreasing activity with time, the H(2)-fed reactor proved to be the most effective in PCE dechlorination: after about 500 days, more than 65% of the added PCE was dechlorinated to ethene in the H(2)-fed reactor, versus 36%, 22%, and <1% in the methanol-fed, butyrate-fed, and control reactors, respectively.

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“…As also explained in Goorissen, Stams and Hansen in a sulphidogenic process of anaerobic metabolism, methanol could be used as an electron donor for sulphur reducing bacteria or degraded by the combined activity of other microorganisms and changed to carbon dioxide. 16 , 18 The same author also stated that its conversion pathway to carbon dioxide in the presence of sulphate or thiosulphate is energetically more favourable than the production of acetate or methane. The results of the NPOC profile ( Figure 2 ) indicated that increasing growth of biomass increased the NPOC value for the first three days.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Methanol Using Thiosulphate as an Electron Acceptor Under Anaerobic Conditions

Tarekegn

2016

Journal of Health and Pollution

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Background.Methanol is a volatile organic compound commonly found in the effluent of the pulp and paper industries. Because of its toxicity, methanol can cause metabolic acidosis, neurologic sequelae, and even death when ingested. Information on biokinetic activity such as biodegradation rate of methanol and thiosulphate, biomass growth rate and biomass yield coefficient is limited in the literature.Objectives.To study the biomass growth rate and biomass yield coefficients of methanol and thiosulphate biodegradation. This research aims to increase knowledge of how to reduce the emission of toxic gas to the environment.Methods.The biodegradation trends of both methanol and thiosulphate were studied under anaerobic conditions using batch experiments at ambient temperature and alkaline conditions. Both supplement each other for their degradation. Methanol is an electron donor, whereas thiosulphate acts as an electron acceptor. A mixed culture from a previously used biomass in a biotrickling filter reactor from theUnited Nations Educational, Scientific and Cultural Organization (UNESCO), International Graduate Water Education Facility and fresh activated sludge from the Harnaschpolder wastewater treatment plant were used as a biomass source.Results.A specific biomass growth rate of biomass ranging from 0.04 to 1.7g per day was observed. The thiosulphate is biologically degraded by the biomass grown inside the reactor. The biodegradation rate of thiosulphate in the reactor varied from 0.02 to 0.80g per unit gram of biomass per day. A biodegradation rate of methanol in the reactor was observed in the range between 0.04 to 3.9g per unit gram of biomass per day. Bacterial biomass was grown as per the amount of methanol present inside the reactor. A maximum biomass yield coefficient of 0.7g biomass per gram of methanol was recorded. Thiosulphate was converted to sulphate that indirectly served as an electron acceptor for methanol degradation. Both degradation of methanol and thiosulphate in this experiment were in the range of the degradation rate shown for sulphate and organic compounds in other studies.Conclusion.Simultaneous removal of thiosulphate and methanol using an anaerobic bioreactor is promising and can be applied on an industrial scale. This finding is an important contribution to public health as it reduces the emission of toxic gas to the environment.

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Methanol utilization in defined mixed cultures of thermophilic anaerobes in the presence of sulfate

Cited by 20 publications

References 21 publications

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

Comparative study of methanol, butyrate, and hydrogen as electron donors for long‐term dechlorination of tetrachloroethene in mixed anerobic cultures

Biodegradation of Methanol Using Thiosulphate as an Electron Acceptor Under Anaerobic Conditions

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