Methanol/Oxygen Enzymatic Biofuel Cell Using Laccase and NAD<sup>+</sup>-Dependent Dehydrogenase Cascades as Biocatalysts on Carbon Nanodots Electrodes

Wu, Guozhi; Gao, Yingming; Zhao, Dan; Ling, Pinghua; Gao, Feng

doi:10.1021/acsami.7b12295

Cited by 40 publications

(29 citation statements)

References 70 publications

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“…Examination of values of P max greater than 1 mW cm -2 ( Table 1) indicates that the majority of high-power-density EFCs are based on carbon nanomaterials (CNMs)-based electrodes. CNMs including buckypaper 46,117,303 , carbon felt 74 , carbon cloth, carbon black 16 , CNTs 62,72,[303][304][305][306][307][308][309][310][311][312][313] , carbon fiber 65,273,[314][315][316][317][318] , graphene 73,93,255,319 , porous carbon 11,63,66,320,321 , carbon nanodots 322 , and their combinations thereof 15,35,185,[323][324][325][326][327] have been widely used for the preparation of bioelectrodes. Although allowing significantly improved current densities, two main challenges of using CNMs based high-surface-area electrodes need to be addressed.…”

Section: Employment Of Nanomaterialsmentioning

confidence: 99%

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

et al. 2019

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The ever-increasing demands for clean and sustainable energy sources combined with rapid advances in bio-integrated portable or implantable electronic devices have stimulated intensive research activities in enzymatic (bio)fuel cells (EFCs). The use of renewable biocatalysts, the utilization of abundant green, safe, and high energy density fuels, together with the capability of working at modest and biocompatible conditions, make EFCs promising as next generation alternative power sources. However, the main challenges (low energy density, relatively low power density, poor operational stability and limited voltage output) hinder future applications of EFCs. This review aims at exploring the underlying mechanism of EFCs and providing possible practical strategies, methodologies and insights to tackle of these issues. Firstly, this review summarizes approaches in achieving high energy densities in EFCs, particularly, employing enzyme cascades for the deep/complete oxidation of fuels. Secondly, strategies for increasing power densities in EFCs, including increasing enzyme activities, facilitating electron transfers, employing nanomaterials, and designing more efficient enzyme-electrode interfaces, are described. The potential of EFCs/(super)capacitor combination is discussed. Thirdly, the review evaluates a range of strategies for improving the stability of EFCs, including the use of different enzyme immobilization approaches, tuning enzyme properties, designing protective matrixes, and using microbial surface displaying enzymes. Fourthly, approaches for the improvement of the cell voltage of EFCs are highlighted. Finally, future developments and a prospective on EFCs are envisioned.

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Section: Employment Of Nanomaterialsmentioning

confidence: 99%

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

et al. 2019

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“…CNDs were prepared according to previous reports [18] [25] [26]. Figure 1 shows the low magnification SEM (Figure 1(A)) and high magnification TEM (Figure 1(B)) images of the obtained CND.…”

Section: Typical Sem and Tem Images Of Carbon Nanodotsmentioning

confidence: 99%

“…In this study, by using GDH-polyMG-CNDs composites as the bioelectrocatalysts for the catalytic electrooxidation of glucose at the bioanode and laccase-CNDs composites as the direct bioelectrocatalyst for oxygen reduction at the biocathode, a membrane-less glucose/air BFC was successfully constructed. The investigations on the direct electron transfer (DET) of blue-copper oxidases including bilirubin oxidase and laccase on CNDs have been studied in our previous studies [18] [26]. These studies have demonstrated that CNDs can efficiently facilitate the DET behaviours of bilirubin oxidase and laccase, and also retain their bioactivity for bio-catalyzing oxygen reduction.…”

Section: Real Applications Of the Fabricated Gdh-polymg-cnds/gc Electmentioning

confidence: 99%

“…These studies have demonstrated that CNDs can efficiently facilitate the DET behaviours of bilirubin oxidase and laccase, and also retain their bioactivity for bio-catalyzing oxygen reduction. Based on previous studies, a laccase-CNDs enzymatic electrode was prepared as before and used as the biocathode for oxygen reduction [18] [26]. In this study, the performances of the constructed glucose/air BFC was further investigated in glucose-containing fruit juice in order to demonstrate its suitability for implantable applications.…”

Section: Real Applications Of the Fabricated Gdh-polymg-cnds/gc Electmentioning

confidence: 99%

“…In this study, a NAD-GDH based enzymatic electrode was fabricated, on which NAD-GDH was immobilized on carbon nanodots (CNDs) with in-situ electrochemical polymerized methylene green (polyMG) on CNDs as electro-oxidation electrocatalyst for NADH, to construct glucose electrochemical biosensor and the bioanode for glucose oxidation in glucose/air BFC. Carbon nanodots were prepared with candle soot as starting material using the reported methods in our previous publications [18] [25] [26]. Typically on the pretreated polyMG-CNDs/GC then let it dry at room temperature, and then 3 μL aqueous solution of nafion (0.5%, wt%) was further coated onto the electrodes to cross-link the enzyme onto modified electrodes.…”

Section: Introductionmentioning

confidence: 99%

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A Glucose-Responsive Enzymatic Electrode on Carbon Nanodots for Glucose Biosensor and Glucose/Air Biofuel Cell

Gao¹,

Wu²,

Gao³

2019

AJAC

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In this study, an enzymatic electrode for glucose biosensing and bioanode of glucose/air biofuel cell has been fabricated by immobilizing poly (methylene green) (polyMG) for electrocatalytic NADH oxidation and NAD + -dependent glucose dehydrogenase (GDH) for oxidizing glucose on carbon nanodots (CNDs). The polyMG-CNDscomposites obtained by electro-polymerization of dye MG molecules adsorbed on CNDs display excellent electrocatalytic activity toward NADH electro-oxidation at a low overpotential of ca. −0.10 V (vs. Ag/AgCl) and the integrated enzymatic electrode shows fast response to glucose electrooxidation. Using the fabricated GDH-based enzymatic electrode, a glucose biosensor was constructed and exhibits a wide linear dynamic range from 0 to 8 mM, a low detection limit of 0.02 μM (S/N = 3), and fast response time (ca. 4 s) under the optimized conditions. The developed glucose biosensor was used to detect glucose content in human blood with satisfactory results. The fabricated GDH-based enzymatic electrode was also employed as bioanode to assembly a glucose/air biofuel cell with the laccase-CNDs/GC as the biocathode. The maximum power density delivered by the assembled glucose/air biofuel cell reaches 3.1 μW•cm −2 at a cell voltage of 0.22 V in real sample fruit juice. The present study demonstrates that potential applications of GDH-based CNDs electrode in analytical and biomedical measurements.

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Implantable Biosupercapacitor Inspired by the Cellular Redox System

et al. 2021

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The carbon nanotube (CNT) yarn supercapacitor has high potential for in vivo energy storage because it can be used in aqueous environments and stitched to inner parts of the body, such as blood vessels. The biocompatibility issue for frequently used pseudocapacitive materials, such as metal oxides, is controversial in the human body. Here, we report an implantable CNT yarn supercapacitor inspired by the cellular redox system. In all living cells, nicotinamide adenine dinucleotide (NAD) is a key redox biomolecule responsible for cellular energy transduction to produce adenosine triphosphate (ATP). Based on this redox system, CNT yarn electrodes were fabricated by inserting a twist in CNT sheets with electrochemically deposited NAD and benzoquinone for redox shuttling. Consequently, the NAD/BQ/CNT yarn electrodes exhibited the maximum area capacitance (55.73 mF cm−2) under physiological conditions, such as phosphate‐buffered saline and serum. In addition, the yarn electrodes showed a negligible loss of capacitance after 10 000 repeated charge/discharge cycles and deformation tests (bending/knotting). More importantly, NAD/BQ/CNT yarn electrodes implanted into the abdominal cavity of a rat's skin exhibited the stable in vivo electrical performance of a supercapacitor. Therefore, these findings demonstrate a redox biomolecule‐applied platform for implantable energy storage devices.

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Methanol/Oxygen Enzymatic Biofuel Cell Using Laccase and NAD⁺-Dependent Dehydrogenase Cascades as Biocatalysts on Carbon Nanodots Electrodes

Cited by 40 publications

References 70 publications

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

A Glucose-Responsive Enzymatic Electrode on Carbon Nanodots for Glucose Biosensor and Glucose/Air Biofuel Cell

Implantable Biosupercapacitor Inspired by the Cellular Redox System

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Methanol/Oxygen Enzymatic Biofuel Cell Using Laccase and NAD+-Dependent Dehydrogenase Cascades as Biocatalysts on Carbon Nanodots Electrodes

Cited by 40 publications

References 70 publications

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

A Glucose-Responsive Enzymatic Electrode on Carbon Nanodots for Glucose Biosensor and Glucose/Air Biofuel Cell

Implantable Biosupercapacitor Inspired by the Cellular Redox System

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Product

Resources

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Methanol/Oxygen Enzymatic Biofuel Cell Using Laccase and NAD⁺-Dependent Dehydrogenase Cascades as Biocatalysts on Carbon Nanodots Electrodes