"Methanol Fraction of Ethanol Extract of Dialium Guineense Stem Bark Mitigates STZ-Induced Oxidative Stress in Rat Liver"

Abstract

The present study investigated the effect of methanol fraction of ethanol extract of Dialium guineense (MEDG) stem bark on streptozotocin (STZ)-induced oxidative stress in rat liver. Male albino rats of Wistar strain (n = 25, mean weight = 215 ± 15 g) were randomly assigned to five groups (5 rats/group): normal control, diabetic control, metformin, MEDG [200 mg/kg body weight (bwt)] and MEDG (300 mg/kg bwt) groups. Diabetes mellitus was induced in the rats via intraperitoneal injection of 50 mg/kg bwt STZ. The diabetic rats were treated for 21 days with either metformin (50 mg/kg bwt) or the extract at doses of 200 and 300 mg/kg bwt, respectively. Activities of antioxidant enzymes such as catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) as well as antioxidant vitamins (retinoic acid, ascorbic acid and α-tocopherol) were measured in 20 % liver homogenate. The results showed that induction of diabetes mellitus with STZ significantly increased the fasting blood glucose (FBG) concentrations of the rats (p < 0.05). However, treatment of the diabetic rats with the extract markedly reduced the blood glucose concentration and body weights of rats (p < 0.05). Treatment of diabetic Wistar rats with MEDG stem bark significantly increased the activities of the antioxidant enzymes and molecules as well as concentrations of nitric oxide (NO), but it markedly reduced the concentrations of hepatic total protein (TP) and malondialdehyde (MDA) (p < 0.05). These results indicate that MEDG stem bark can potentiate antioxidant defense in diabetic rat liver.

"Protective Property of Ethanol Extract of C. Sativus on STZ-Induced Diabetic Rat Pancreas"

“…However, treatment of the diabetic Wistar albino rats with two different doses of ethanol extract of C. sativus fruit markedly reduced the FBG concentration and body weights of rats, but enhanced the activity/concentration of antioxidant enzymes/molecules in pancreatic tissue. These results are in agreement with those of previous studies [28][29][30][31][32][33]. The results suggest that oxidative stress plays a crucial role in STZ-induced diabetes mellitus and that increased ROS production suppresses the activity of antioxidant enzymes, while diminishing the levels of antioxidant molecules in pancreatic tissue.…”