Methanol extracts of Xanthium sibiricum roots inhibit inflammatory responses via the inhibition of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) in murine macrophages

Ju, Anna; Cho, Young-Chang; Cho, Sayeon

doi:10.1016/j.jep.2015.07.038

Cited by 25 publications

(14 citation statements)

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“…This compound has been reported to possess various kinds of biological properties, including anti-inflammatory, anticancer, antimalarial, and antiplatelet activities, etc. ( Lee et al, 2011 ; Wan et al, 2013 ; Jingbo et al, 2015 ; Ju et al, 2015 ; Lingaraju et al, 2015 ; Kim et al, 2016 ; Laavola et al, 2016 ). The anti-inflammatory effect of BA is well recognized in several different models ( Saaby and Nielsen, 2012 ; Wan et al, 2013 ; Ju et al, 2015 ; Lingaraju et al, 2015 ; Kim et al, 2016 ; Laavola et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…( Lee et al, 2011 ; Wan et al, 2013 ; Jingbo et al, 2015 ; Ju et al, 2015 ; Lingaraju et al, 2015 ; Kim et al, 2016 ; Laavola et al, 2016 ). The anti-inflammatory effect of BA is well recognized in several different models ( Saaby and Nielsen, 2012 ; Wan et al, 2013 ; Ju et al, 2015 ; Lingaraju et al, 2015 ; Kim et al, 2016 ; Laavola et al, 2016 ). First, BA could inhibit LPS-stimulated release of inflammatory cytokines in RAW 264.7 macrophages and protect against animal death and tissue damage during LPS/polymicrobial-induced sepsis ( Lingaraju et al, 2015 ; Kim et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effects of Betulinic Acid on LPS-Induced Neuroinflammation Involve M2 Microglial Polarization via CaMKKβ-Dependent AMPK Activation

Zhang

Zhou

et al. 2018

Front. Mol. Neurosci.

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In response to the microenvironment, microglia may polarize into either an M1 pro-inflammatory phenotype, exacerbating neurotoxicity, or an M2 anti-inflammatory phenotype, conferring neuroprotection. Betulinic acid (BA) is a naturally pentacyclic triterpenoid with considerable anti-inflammatory properties. Here, we aim to investigate the potential effects of BA on microglial phenotype polarization and to reveal the underlying mechanisms of action. First, we confirmed that BA promoted M2 polarization and inhibited M1 polarization in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Then, we demonstrated that the effect of BA on microglial polarization was dependent on AMP-activated protein kinase (AMPK) activation, as evidenced by the fact that both AMPK inhibitor compound C and AMPK siRNA abolished the M2 polarization promoted by BA. Moreover, we found that calmodulin-dependent protein kinase kinase β (CaMKKβ), but not liver kinase B1, was the upstream kinase required for BA-mediated AMPK activation and microglial M2 polarization, via the use of both the CaMKKβ inhibitor STO-609 and CaMKKβ siRNA. Finally, BA enhanced AMPK phosphorylation and promoted M2 microglial polarization in the cerebral cortex of LPS-injected mice brains, which was attenuated by pre-administration of the AMPK inhibitor. This study demonstrated that BA promoted M2 polarization of microglia, thus conferring anti-neuroinflammatory effects via CaMKKβ-dependent AMPK activation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Betulinic Acid on LPS-Induced Neuroinflammation Involve M2 Microglial Polarization via CaMKKβ-Dependent AMPK Activation

Zhang

Zhou

et al. 2018

Front. Mol. Neurosci.

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“…b-Sitosterol and stigmasterol are common plant sterols, and have been widely studied over the last 15 years. Several studies show that these phytosterols have anti-inflammatory activity in carrageenan and TPA models, as well as reducing myeloperoxidase activity and pro-inflammatory cytokines (IL-1b, IL-6 and TNF-a) (Navarro et al 2001;Ammar et al 2009;Nirmal et al 2012;Hernandez-Valle et al 2014;Ju et al 2015). We found that compound B Figure 2.…”

Section: Resultsmentioning

confidence: 65%

Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents

Jiménez-Suárez

Nieto‐Camacho

Manuel

et al. 2016

Pharmaceutical Biology

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Context Hamelia patens Jacq. (Rubiaceae) is traditionally used to treat wounds, inflammation and diabetes. However, there is still a lack of scientific evidence to support these applications. Objective The objective of this study is to evaluate the anti-inflammatory, antioxidant and antidiabetic activities of Hamelia patens, and identify its bioactive compounds. Materials and methods Four extracts were obtained by maceration and liquid-liquid extraction: HEX, DCM-EtOAc, MeOH-EtOAc and MeOH-Aq. The anti-inflammatory effect was evaluated orally on rat paw carrageenan-induced oedema over 6 h (50, 200 and 500 mg/kg), and topically in mouse ear oedema induced by 12-tetradecanoylphorbol-13-acetate (TPA) after 4 h (0.5 and 1 mg/ear). We also evaluated myeloperoxidase levels in ear tissue, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability, and in vitro α-glucosidase inhibition. The chemical compounds were separated by column chromatography and identified by spectroscopic analysis. Results We found that the oral administration of the HEX extract at 500 and 200 mg/kg significantly decreased the carrageenan-induced inflammation after 1 and 3 h, respectively. The MeOH-EtOAc extract significantly inhibited myeloperoxidase activity (83.5%), followed by the DCM-EtOAc extract (76%), β-sitosterol/stigmasterol (72.7%) and the HEX extract (55%), which significantly decreased oedema induced by TPA at both doses, giving a similar effect to indomethacin. We also found that the MeOH-EtOAc, MeOH-Aq and DCM-EtOAc extracts showed good DPPH scavenging activity (IC50 values of 18.6, 93.9 and 158.2 μg/mL, respectively). The HEX extract showed the lowest α-glucosidase inhibition (an IC50 value of 26.07 μg/mL), followed by the MeOH-EtOAc extract (an IC50 value of 30.18 μg/mL), β-sitosterol/stigmasterol (IC50 34.6 μg/mL) and compound A ((6E,10E,14E,18E)-2,6,10,14,18,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, an IC50 value of 114.6 μg/mL), which were isolated for the first time from Hamelia patens. Discussion and conclusion Hamelia patens possesses anti-inflammatory, antioxidant and α-glucosidase inhibitory activities, which support its traditional use. These effects can be attributed to the identified compounds.

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“…LPS는 대표적인 pattern recognition receptor (패턴 인식 수용체)인 toll-like receptor 4와 MD-2 복합체에 의해 인식되며 그 결과, 세포 내 주요 신호전달 경로 중 하나인 mitogen-activated protein kinase (MAPK) family 를 활성화 시키게 된다 [2]. 이를 구성하는 extracellular signal-regulated kinase 1/2 (ERK1/2), jun N-terminal kinase (JNK) 그리고 p38은 전사 조절 인자인 ATF2 [5], CREB [9], STAT3 [3] 그리 고 NF-κB [19]와 같은 다양한 전사조절인자들을 통해 inducible nitric oxide synthase (iNOS) 및 TNF-alpha [7], cyclooxygenase-2 (COX-2) 그리고 cytokine [20] Mouse RAW 264.7 cells were treated with 100 μg/ml or 200 μg/ml of KSD-E8-3 and total proteins were prepared from treated cells and Western blotting was carried out by using p38, phospho-p38, JNK, phospho-JNK, ERK1/2, phospho-ERK1/2, and Actin antibodies. …”

Section: 서 론 염증(Inflammation)은 다양한 외부 자극으로 인해 발생하 는 신체 방어 기작으로서[1] Lpunclassified

Suppressive Effects of Lees from Sweet Potato Soju on LPS-induced Inflammatory Responses in RAW 264.7 Cells

Lee¹,

Kwon²,

Kim³

et al. 2016

Journal of Life Science

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In the current study, the ethanol extracts and their subsequent organic solvent fractions from lees of sweet potato soju were prepared and the prepared samples were designated as from KSD-E8-1 to KSD-E8-5. Their effects on cell viability and nitric oxide (NO) production in mouse macrophage RAW 264.7 cells were investigated. The results showed that the ethyl acetate fraction (KSD-E8-3) of lees extracts from sweet potato soju significantly decreased nitric oxide (NO) production in LPS-activated RAW 264.7 cells, whereas they did not affect cell viabilities. The fraction KSD-E8-3 reduced the expression of pro-inflammatory genes such as COX-2, iNOS and TNF-alpha and also decreased protein expression of iNOS in a dose dependent manner, which were detected with RT-PCR and Western blot analysis, respectively. In addition, we detected the expression of mitogen-activated protein kinases (MAPKs) such as p38, JNK, and ERK1/2 and their phosphorylated forms. The results indicated that the treatment of the fraction KSD-E8-3 did inhibit phosphorylation of p38, JNK, and ERK1/2 MAPKs, indicating that the fraction KSD-E8-3 regulates LPS-induced inflammatory response via suppressing MAPK signaling pathway. Overall, these results may contribute to understand the molecular mechanism of anti-inflammatory effects by the ethyl acetate fraction of lees extracts from sweet potato soju.

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Methanol extracts of Xanthium sibiricum roots inhibit inflammatory responses via the inhibition of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) in murine macrophages

Cited by 25 publications

References 54 publications

Inhibitory Effects of Betulinic Acid on LPS-Induced Neuroinflammation Involve M2 Microglial Polarization via CaMKKβ-Dependent AMPK Activation

Inhibitory Effects of Betulinic Acid on LPS-Induced Neuroinflammation Involve M2 Microglial Polarization via CaMKKβ-Dependent AMPK Activation

Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents

Suppressive Effects of Lees from Sweet Potato Soju on LPS-induced Inflammatory Responses in RAW 264.7 Cells

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