Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro

Marković, Aleksandra; Vukajlović, Jovana Tubić; Grujičić, Darko; Jakovljević, Marina Radović; Stanković, Milan; Djordjević, Katarina; Djelić, Ninoslav; Radaković, Milena; Milošević-Djordjević, Olivera

doi:10.1080/01480545.2020.1802477

Cited by 2 publications

(4 citation statements)

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“…Markovic et al . also showed that 125, 250, 500 and 1000 μg mL −1 Teucrium arduini and 1000 μg mL −1 Teucrium flavum (Lamiaceae) methanol extracts significantly reduced the mitomycin C‐induced micronucleus frequency in human peripheral blood lymphocytes 17 . In another study, Thymbra spicata L. (Lamiaceae) extracts (water, methanol and ethanol extracts) at different concentrations (25, 50, 100 and 200 mg L −1 ) showed antigenotoxic effects against HgCl 2 18 .…”

Section: Resultsmentioning

confidence: 89%

“…16 Markovic et al also showed that 125, 250, 500 and 1000 μg mL −1 Teucrium arduini and 1000 μg mL −1 Teucrium flavum (Lamiaceae) methanol extracts significantly reduced the mitomycin C-induced micronucleus frequency in human peripheral blood lymphocytes. 17…”

Section: Micronucleus Assay Resultsmentioning

confidence: 99%

“…Many compounds and complex mixtures such as food additives, beverages and insecticides have been tested by SMART 15 . Thus, there are many studies in the literature about the interactions of mutagens with various plants deciphered by micronucleus 16‐19 and SMART assays 14,20 …”

Section: Introductionmentioning

confidence: 99%

“…14 Many compounds and complex mixtures such as food additives, beverages and insecticides have been tested by SMART. 15 Thus, there are many studies in the literature about the interactions of mutagens with various plants deciphered by micronucleus [16][17][18][19] and SMART assays. 14,20 Hydrogen peroxide (H 2 O 2 ) is a well-known inducer of cancer in animals because of its mutagenic potential and it is a natural source of oxidative damage in cells, causing a spectrum of DNA lesions, including single-and double-strand breaks.…”

Section: Introductionmentioning

confidence: 99%

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Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

et al. 2022

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BACKGROUND Plants have important chemical compounds to protect organisms from many mutagens but they may also have dangerous toxic substances that must be tested before use. Lallemantia iberica has been used by local people for treatment of some diseases but it was never tested for its cell protective and antimutagenic/antirecombinogenic properties. In this study, it was aimed to determine the cytoprotective, antimutagenic/antirecombinogenic, antibacterial, antioxidant and phenolic profile of L. iberica by MTS assay, micronucleus test/somatic mutation recombination test (SMART), disc diffusion method, ABTS/DPPH tests and liquid chromatography (LC), respectively. RESULTS MTS assay results showed that both of the extracts supported the normal growth of healthy human lymphocytes and there was no significant difference between extracts. Although there was no significant increase in micronucleus concentration (‰) of the cultures treated with ethanol or methanol extracts, methanol extract caused slightly lower micronucleus concentration (12.64 ± 3.65‰) than the ethanol extract (24.46 ± 4.50‰). SMART results showed that ethanol and methanol extracts prevented harmful mitotic recombination. Lallemantia iberica also showed antibacterial activity against all of the strains tested, and the largest inhibition zones were observed with Pseudomonas aeruginosa (17.9 mm) and Enterobacter aerogenes (13.8 mm). Methanol extracts showed better antioxidant activities (ABTS IC50: 104.07 μg mL−1, DPPH IC50: 58.8 μg mL−1) than ethanol extracts (ABTS IC50: 344.03 μg mL−1, DPPH IC50: 233.7 μg mL−1). According to the LC results, the most abundant phenolic compounds were rutin hydrate (1796.4 ± 62.9 mg kg−1) and p‐coumaric acid (228.1 ± 7.60 mg kg−1). CONCLUSION Lallemantia iberica extracts had cell‐protective, antimutagenic/antirecombinogenic, antibacterial and strong antioxidant characteristics in relation to their rich phenolic content. © 2022 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 89%

Section: Micronucleus Assay Resultsmentioning

confidence: 99%