Methanogens: biochemical background and biotechnological applications

Enzmann, Franziska; Mayer, Florian; Rother, Michael; Holtmann, Dirk

doi:10.1186/s13568-017-0531-x

Cited by 333 publications

(157 citation statements)

References 210 publications

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“…The high iRep values measured here for some known species also suggests that their isolation may be easier as previously assumed [55]. The low replication rate of Euryarchaeota (~1.45 on average) is also known [56], but in the present study, comparative data for 17 MAGs were reported extending previous findings and providing results for multiple species having different abilities in substrates utilization. In particular, the Euryarchaeota with the highest iRep is Methanothrix soehngenii AS27yjCOA_157, followed by Methanomicrobiales sp.…”

Section: Resultssupporting

confidence: 86%

The anaerobic digestion microbiome: a collection of 1600 metagenome-assembled genomes shows high species diversity related to methane production

Campanaro

Treu

Rodriguez-R

et al. 2019

Preprint

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29Background 30 Microorganisms in biogas reactors are essential for degradation of organic matter and methane 31 production through anaerobic digestion process. However, a comprehensive genome-centric 32 comparison, including relevant metadata for each sample, is still needed to identify the globally 33 distributed biogas community members and serve as a reliable repository. 34 Results 35Here, 134 publicly available datasets derived from different biogas reactors were used to recover 36 1,635 metagenome-assembled genomes (MAGs) representing different bacterial and archaeal 37 species. All genomes were estimated to be >50% complete and nearly half were ≥90% complete 38 with ≤5% contamination. In most samples, specialized microbial communities were established, 39 while only a few taxa were widespread among the different reactor systems. Metabolic 40 reconstruction of the MAGs enabled the prediction of functional traits related to biomass 41 degradation and methane production from waste biomass. An extensive evaluation of the replication 42 index provided an estimation of the growth rate for microbes involved in different steps of the food 43 chain. The recovery of many MAGs belonging to Candidate Phyla Radiation and other 44 underexplored taxa suggests their specific involvement in the anaerobic degradation of organic 45 matter. 46 Conclusions 47 The outcome of this study highlights a high flexibility of the biogas microbiome. The dynamic 48 composition and adaptability to the environmental conditions, including temperatures and a wide 49 range of substrates, were demonstrated. Our findings enhance the mechanistic understanding of 50 anaerobic digestion microbiome and substantially extend the existing repository of genomes. The 51 established database represents a relevant resource for future studies related to this engineered 52 ecosystem. 53 3 54Background 55 Anaerobic environments are ubiquitous in the biosphere, some examples are the digestive tract of 56 animals, paddy fields and aquatic sediments. These environments play crucial roles in the 57 degradation of organic matter and in the global carbon cycle [1,2]. The anaerobic digestion (AD) 58 process is also an example of such an environment, having great societal importance since it 59 reduces our dependence on fossil fuels via its ability to generate methane within engineered 60 bioreactors [3]. For these reasons, the AD process has been widely acknowledged as an efficient 61 biochemical route allowing the conversion of organic wastes into energy and other valuable 62 products, and has been posited as a sustainable solution for resource recovery and renewable energy 63 production underpinning the circular economy concept [4]. Apart from biowaste valorization, this 64 process is also of great importance for nutrient recycling, since it stabilizes the nitrogen compounds 65 making them more easily assimilable by plants, reducing nitrogen leakage to soil and groundwater. 66 Methane is one of the most relevant end-products generated during the meth...

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Section: Resultssupporting

confidence: 86%

The anaerobic digestion microbiome: a collection of 1600 metagenome-assembled genomes shows high species diversity related to methane production

Campanaro

Treu

Rodriguez-R

et al. 2019

Preprint

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“…[−] (29) As temperature and pressure of the gas volume flows are not considered, the efficiency calculation can be simplified by using lower heating values (LHVs) instead of the enthalpy flows (Equation (30)):…”

Section: Q Lossmentioning

confidence: 99%

“…However, a major restriction for the chemical methanation is the requirement of high reactant gas purities because of the sensitivity of the metal catalyst towards contaminants such as hydrogen sulfide [20,22,24,26]. Biological methanation uses biological catalysts i.e., methanogenic microorganisms to catalyze the methanation reaction [27][28][29]. As a consequence, reactors work Energies 2019, 12, 1670 3 of 32 normally at temperatures between 37 and 65 • C and pressures from one to 15 bars to meet the optimal growth conditions of these microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Biological CO2-Methanation: An Approach to Standardization

Thema

Weidlich

Hörl³

et al. 2019

Energies

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Power-to-Methane as one part of Power-to-Gas has been recognized globally as one of the key elements for the transition towards a sustainable energy system. While plants that produce methane catalytically have been in operation for a long time, biological methanation has just reached industrial pilot scale and near-term commercial application. The growing importance of the biological method is reflected by an increasing number of scientific articles describing novel approaches to improve this technology. However, these studies are difficult to compare because they lack a coherent nomenclature. In this article, we present a comprehensive set of parameters allowing the characterization and comparison of various biological methanation processes. To identify relevant parameters needed for a proper description of this technology, we summarized existing literature and defined system boundaries for Power-to-Methane process steps. On this basis, we derive system parameters providing information on the methanation system, its performance, the biology and cost aspects. As a result, three different standards are provided as a blueprint matrix for use in academia and industry applicable to both, biological and catalytic methanation. Hence, this review attempts to set the standards for a comprehensive description of biological and chemical methanation processes.

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“…In general, 298 methanogens tend to grow more slowly than denitrifers. It could be that the mcrA response was not as 299 strong due to insufficient time to increase in abundance (Enzmann et al, 2018). Another interesting aspect 300 of Cu effects on CH 4 emissions is the competition between methanotrophs and denitrifiers for the "Cu 301 monopoly" (Chang et al, 2018), which should be further investigated in environmental samples.…”

Section: Effect On Carbon Mineralization 279mentioning

confidence: 99%

Trace metal availability affects greenhouse gas emissions and microbial functional group abundance in freshwater wetland sediments

Giannopoulos

Hartop

Brown

et al. 2019

Preprint

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We investigated the effects of trace metal additions on microbial nitrogen and carbon cycling using freshwater wetland sediment microcosms amended with μM concentrations of copper (Cu), molybdenum (Mo), iron (Fe), and all combinations. In addition to monitoring inorganic nitrogen transformations (NO3−, NO2−, N2O, NH4+) and carbon mineralization (CO2, CH4), we tracked changes in functional gene abundance associated with denitrification (nirS, nirK, nosZ), DNRA (nrfA), and methanogenesis (mcrA). Greater availability of Cu led to more complete denitrification (i.e., less N2O accumulation) and a higher abundance of the nirK and nosZ genes, which encode for Cu-dependent reductases. We found sparse evidence of DNRA activity and no consistent effect on CO2 production. Contrary, net CH4 production was stimulated by the trace metal amendments and the Mo additions, in particular, led to increased mcrA gene abundance. Taken together, these findings demonstrate that trace metal effects on microbial physiology, which have heretofore only been studied in pure culture, can impact community-level function. We observed direct and indirect effects on both nitrogen and carbon biogeochemistry that culminated in increased production of greenhouse gasses, and the shifts in functional group abundance that we documented suggest these responses may have been mediated through changes in microbial community composition. Overall, this work supports a more holistic consideration of metal effects on environmental microbial communities that recognizes the key role that metal limitation plays in microbial physiology.

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Methanogens: biochemical background and biotechnological applications

Cited by 333 publications

References 210 publications

The anaerobic digestion microbiome: a collection of 1600 metagenome-assembled genomes shows high species diversity related to methane production

The anaerobic digestion microbiome: a collection of 1600 metagenome-assembled genomes shows high species diversity related to methane production

Biological CO2-Methanation: An Approach to Standardization

Trace metal availability affects greenhouse gas emissions and microbial functional group abundance in freshwater wetland sediments

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