Methanobactin: A Novel Copper-Binding Compound Produced by Methanotrophs

Semrau, Jeremy D.; DiSpirito, Alan A.

doi:10.1007/978-3-030-23261-0_7

Cited by 2 publications

(2 citation statements)

References 152 publications

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“…Methanobactins (Mbns) are peptide chalkophores that bind copper, which is needed for cell synthesis [79]. They were originally characterized in the methanotrophic bacterial species Methylococcus capsulatus and were found to be required for the functioning of methane oxidizing pMMO [68], which is transported outside of the cell to trap and bind to Cu(I) ions [80]. This chelating effect also reduces Cu(II), which is toxic to the bacterial cell, and to Cu(I) in copper-deficient environments [81,82].…”

Section: Methanobactinmentioning

confidence: 99%

“…This is especially important as the ability of reducing gold has successfully generated gold nanoparticles that have many biomedical applications. These applications include antimicrobial activity, incorporation in fuel cells, photo-thermal and dynamic therapy, cancer treatment, act as an epitope, and tether to immunogenic molecules that have the ability to attach to cancer biomarkers (antigens), and in the development of many medically advanced technologies such as plasmonic biosensors, for visualization, and bioimaging [80]. Mbns also show great potential in the treatment of copper-associated illnesses such as Wilson disease (WD), Alzheimer disease (AD), fatty liver disease, and BRAF-positive cancers by preventing copper buildup in the liver and consequently other body tissues, and thereby preventing permanent liver and neurological tissue damage [83].…”

Section: Methanobactinmentioning

confidence: 99%

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Biomedical Applications of Biomolecules Isolated from Methanotrophic Bacteria in Wastewater Treatment Systems

2021

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Wastewater treatment plants and other remediation facilities serve important roles, both in public health, but also as dynamic research platforms for acquiring useful resources and biomolecules for various applications. An example of this is methanotrophic bacteria within anaerobic digestion processes in wastewater treatment plants. These bacteria are an important microbial source of many products including ectoine, polyhydroxyalkanoates, and methanobactins, which are invaluable to the fields of biotechnology and biomedicine. Here we provide an overview of the methanotrophs’ unique metabolism and the biochemical pathways involved in biomolecule formation. We also discuss the potential biomedical applications of these biomolecules through creation of beneficial biocompatible products including vaccines, prosthetics, electronic devices, drug carriers, and heart stents. We highlight the links between molecular biology, public health, and environmental science in the advancement of biomedical research and industrial applications using methanotrophic bacteria in wastewater treatment systems.

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Section: Methanobactinmentioning

confidence: 99%

Section: Methanobactinmentioning

confidence: 99%

Biomedical Applications of Biomolecules Isolated from Methanotrophic Bacteria in Wastewater Treatment Systems

2021

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Methanobactin from methanotrophs: genetics, structure, function and potential applications

Semrau

DiSpirito

Obulisamy

et al. 2020

FEMS Microbiology Letters

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Aerobic methane-oxidizing bacteria of the Alphaproteobacteria have been found to express a novel ribosomally synthesized post-translationally modified polypeptide (RiPP) termed methanobactin (MB). The primary function of MB in these microbes appears to be for copper uptake, but MB has been shown to have multiple capabilities, including oxidase, superoxide dismutase and hydrogen peroxide reductase activities, the ability to detoxify mercury species, as well as acting as an antimicrobial agent. Herein, we describe the diversity of known MBs as well as the genetics underlying MB biosynthesis. We further propose based on bioinformatics analyses that some methanotrophs may produce novel forms of MB that have yet to be characterized. We also discuss recent findings documenting that MBs play an important role in controlling copper availability to the broader microbial community, and as a result can strongly affect the activity of microbes that require copper for important enzymatic transformations, e.g. conversion of nitrous oxide to dinitrogen. Finally, we describe procedures for the detection/purification of MB, as well as potential medical and industrial applications of this intriguing RiPP.

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Methanobactin: A Novel Copper-Binding Compound Produced by Methanotrophs

Cited by 2 publications

References 152 publications

Biomedical Applications of Biomolecules Isolated from Methanotrophic Bacteria in Wastewater Treatment Systems

Biomedical Applications of Biomolecules Isolated from Methanotrophic Bacteria in Wastewater Treatment Systems

Methanobactin from methanotrophs: genetics, structure, function and potential applications

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