Methanobacterium thermoalcaliphilum spec. nov., a new moderately alkaliphilic and thermophilic autotrophic methanogen

Blotevogel, Karl-Heinz; Fischer, Ulrich; Mocha, Margit; Jannsen, Sigrid

doi:10.1007/bf00693392

Cited by 59 publications

(18 citation statements)

References 22 publications

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“…H .~ and M.0.H.G also indicate that strain G B T is a member of the genus Methanobacterium, but not necessarily a strain of the species Methanobacterium bryantii. These results support the identification of strain GP9T as a Methanobacterium species; however, strain GP9T is a mesophile and therefore differs fionnthe thermophilic species (2,3,26,28) of the genus Methanobacterium. Strain GP9T also differs from the mesophilic species Methanobacterium formicicum and Methanobacterium uliginosum in its growth pH requirements, growth substrates, and DNA base composition (12,23).…”

Section: Discussionsupporting

confidence: 81%

“…The DNA base composition of strain GP9T is in the range (30 to 50 mol% G+C) usually observed for species of the genus Methanobacterium (2,3,12,23). The morphology and indirect immunofluorescence reaction of strain GP9T indicate that it is more closely related to members of the genus Methanobacterium than to members of the genus Methanobrevibacter.…”

Section: Discussionmentioning

confidence: 80%

“…Similar to strain GP9T, there are other methanogens that can use only H,-CO, as a growth and methanogenic substrate (3,7,26,27). Like the recently described organism "Methanobacterium palustre" and Methanobacterium bryantii M .…”

Section: Discussionmentioning

confidence: 94%

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Isolation and Characterization of Methanobacterium espanolae sp. nov., a Mesophilic, Moderately Acidiphilic Methanogen

Patel

Sprott

Fein³

1990

International Journal of Systematic Bacteriology

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Bacterial strain G n T (T = type strain), a nonmotile, nonsporeforming, mesophilic, methanogenic bacterium, was isolated from the primary sludge obtained from the waste treatment facility of a major kraft pulp mill in Canada. Single cells were 6.0 by 0.8 pm and stained gram positive. Growth and methane production occurred only with H,-CO, as the substrate. Acetate, formate, propionate, butyrate, pyruvate, methanol, or trimethylamine could not serve as a sole source of carbon and energy for growth. The optimum pH for growth was between 5.6 and 6.2; consistent growth and methane production were not observed below pH 4.68. The optimum temperature for growth was 35"C, and little or no growth was observed during incubation at 15 and 50°C. Kanamycin and bacitracin were severe inhibitors of growth and methanogenesis, whereas 100 pM bromoethanesulfonic acid caused 30 % inhibition. Supernatant from primary sludge enhanced growth by about 10%. The DNA base composition was 34 mol% guanine plus cytosine. On the basis of physiological characteristics, indirect immunofluorescence typing, and DNA-DNA hybridization studies, the isolate is named Methanobacterium espanolae sp. nov.The optimum pH for growth of most pure cultures of methanogenic bacteria isolated to date lies between 6.0 and 8.0, with the majority of cultures having an optimum close to pH 7.0 (23,25). Methanogenic bacteria with optimum growth pH values greater than 8.0 have been reported recently (27). Although methanogenic activity has been detected in acidic ecosystems, such as bogs and peats (11,24,25), at pH values close to 4.0, methanogenic isolates from these ecosystems have been unable to grow at pH values below 5.3 (25). Moreover, no pure culture of a methanogen that is able to grow at pH values below 5.0 has been reported previously, to our knowledge. The isolation and study of acid-tolerant methanogens was undertaken as part of a program on biomass degradation in anaerobic, acidic environments, with the purpose of extending the potential of anaerobic treatment for disposal of acidic, organic, industrial wastes. In this paper we describe the isolation and characterization of a methanogen that is able to grow and produce methane at pH values below 5.0. MATERIALS AND METHODSMedia. The composition of SA medium, which was used for isolation and stock culture maintenance, was as described previously (5) except for an increase in the cysteineNa,S concentration. This medium contained (per liter) 2,500 mg of CH,COONa -3H@, 480 mg of Na,CO,, 450 mg of mg of vitamin B12. The pH of the mixed ingredients (except cysteine-Na,S and Na,CO,) was adjusted to 4.5 with 5 N HC1. The medium was then reduced with cysteine-Na,S, Na,CO, was added, and 10-ml portions were dispensed into 60-ml serum vials under 80% H2-20% CO, (5). The postautoclaved (121"C, 15 min) pH of the medium was 5.5 k 0.1. Similarily, SA medium with an initial pH of 5.0 and S medium (the same as SA medium but without acetic acid) with pH values of 5.5 and 5.0 were prepared by appropriate pH adjustment before...

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 80%

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Isolation and Characterization of Methanobacterium espanolae sp. nov., a Mesophilic, Moderately Acidiphilic Methanogen

Patel

Sprott

Fein³

1990

International Journal of Systematic Bacteriology

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“…However, these four strains differ from previously described species of the genus Methanobacterium by their lack of immunological cross-reactions, their high pH range for growth, and the high guanine-plus-cytosine contents of their deoxyribonucleic acids (Boone et al, in press). Unlike other species of Methanobacterium, our four strains stained gram negative; two other isolates ("Methanobacterium thermoaggregans" [2] and l 'Methanobacterium thermoalcaliphilum' ' [3]) appear to be gram-negative bacteria which belong to the genus Methanobacterium. We propose a new species, Methanobacterium alcaliphilum, for our isolates, with strain WeN4 as the type strain.…”

Section: Resultscontrasting

confidence: 53%

“…Recently, several alkaliphilic strains were isolated, including the thermophilic organism "Methanobacterium thermoalcaliphilicum" (3) and four mesophilic isolates from the Wadi el Natrun in Egypt (D. R. Boone, S . Worakit, I. M. Mathrani, and R. A.…”

mentioning

confidence: 99%

Methanobacterium alcaliphilum sp. nov., an H2-Utilizing Methanogen That Grows at High pH Values

Worakit

Boone

Mah

et al. 1986

International Journal of Systematic Bacteriology

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Four strains of alkaliphilic methanogens (strains WeN1, WeN2, WeN3, and WeN4T [T = type strain]) previously enriched and isolated from sediments of four low-salt, high-pH (pH 8.3 to 9.3) lakes in the Wadi el Natrun of Egypt were further characterized. These organisms were H2-oxidizing, CO2-reducing, rod-shaped bacteria which grew best at high pH values (strains WeN1, WeN2, and WeN4T preferred pH 8.4 and grew at pH values up to 9.9; strain WeN3 grew best at pH 7.8 and grew at pH values up to 9.2). Each strain required sulfide as a sulfur source and was resistant to elevated concentrations (growth rates in the presence of 0.5% NazS * 9H20 were one-third to one-half the maximum observed rates). The guanine-plus-cytosine content of the deoxyribonucleic acid of strain WeN4T was 57 mol%. A new species of methanogen, Methanobacterium alcaliphilum, is described; strain WeN4 (= DSM 3387) is the type strain.Most previously studied methanogens were isolated from habitats with pH values near neutrality, and such pH values support the best growth of these microbes. Recently, several alkaliphilic strains were isolated, including the thermophilic organism "Methanobacterium thermoalcaliphilicum" (3) and four mesophilic isolates from the Wadi el Natrun in Egypt (D. R. Boone, S . Worakit, I. M. Mathrani, and R. A.Mah, Syst. Appl. Microbiol., in press).We report here further characterization of the four mesophilic strains, which belong to a new species of bacterium, Methanobacterium alcaliphilum sp. nov. MATERIALS AND METHODSInocula and media. Sediment samples were collected from four Egyptian lakes of the Wadi el Natrun (Boone et al., in press). The culture media used contained (per liter of deionized water) 10 g of NaHC03, 2.0 g of yeast extract, 2.0 g of Trypticase peptone, 1 g of NH4C1, 0.5 g of L-cysteine hydrochloride, 0.1 g of Mg2C1 . 6H20, 0.02 g of CaC12, 0.4 g of K2HP04, 1 mg of resazurin, 0.1 mg of H2Se03, 1 mg of MnC12 -4H20, 1 mg of FeS04 7H20, 1.5 mg of CoCl2 -6H20, 1 mg of ZnC12, 0.1 mg of H3B03, 0.1 mg of NaMo04.2H20, 0.2 mg of CuC12. 2H20, 0.2 mg of NiS04 -6H20, 0.4 mg of A1C13 -6H20, and 5 mg of disodium ethylenediaminetetraacetic acid dihydrate. The gas phase was 100% N2. All ingredients except NaHC03, yeast extract, Trypticase peptone, and L-cysteine hydrochloride were dissolved in 1,020 ml of deionized water, which was boiled under a stream of 02-free nitrogen. Trypticase peptone, yeast extract, ,and L-cysteine hydrochloride were added; for solid media, 18 g of purified agar was also added. Boiling was continued until the volume was reduced to 1,000 ml. The medium was cooled, and 02-free NaHC03 was added. The NaHC03 was rendered 02-free by storage in an anaerobic chamber or in a sealed tube flushed with N2 gas. The medium was distributed to serum tubes, which were sealed with butyl rubber stoppers secured with aluminum * Corresponding author. t Present address: Biological Sciences Division, The Office of Atomic Energy for Peace, Thanon Viphavadee Rungsit, Bangkhen, Bangkok 10900, Thailand. crimp closures and a...

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Thermophiles: Anaerobic Alkalithermophiles

Wiegel

2003

Encyclopedia of Environmental Microbiology

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Definition of “Extreme” Mesophilic and Thermophilic Alkaliphiles have been Studied Only Recently Alkalithermophiles Thermophilic “Superbugs” Among Validly Described Species of Extremophiles Biogeography and Biogeochemistry Some Comments on Methods for Studying Anaerobic Alkalithermophiles Anaerobic Alkalithermophilic Bacteria and their Habitats Known Thus Far Archaeal Species and their Known Habitats Possible Correlation between the Shortest Observed Doubling Times for a Species and the Type of Habitat from Which the Alkalithermophiles were Isolated

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Methanobacterium thermoalcaliphilum spec. nov., a new moderately alkaliphilic and thermophilic autotrophic methanogen

Cited by 59 publications

References 22 publications

Isolation and Characterization of Methanobacterium espanolae sp. nov., a Mesophilic, Moderately Acidiphilic Methanogen

Isolation and Characterization of Methanobacterium espanolae sp. nov., a Mesophilic, Moderately Acidiphilic Methanogen

Methanobacterium alcaliphilum sp. nov., an H2-Utilizing Methanogen That Grows at High pH Values

Thermophiles: Anaerobic Alkalithermophiles

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