Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters

Abstract

Two methanogenic strains, 8-2T and 4-1, with rod-shaped (0?4-0?563-5 mm), non-motile cells, sometimes observed in chains, were isolated from two anaerobic digesters in Beijing, China. The two strains used H 2 /CO 2 and formate for growth and produced methane. The temperature range for growth was 25-50 6C, with fastest growth at 37 6C. The pH ranges for growth and methane production were 6?5-8?0 for strain 8-2 T and 6?8-8?6 for strain 4-1, with the fastest growth at pH 7?2 for strain 8-2 T and pH 7?5-7?7 for strain 4-1. The G+C content of genomic DNA for strain 8-2 T was 38?9 mol%. The similarity levels of the 16S rRNA

Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake

“…The cultivated, recognized species that appeared most closely related to strain 17A1 T , with a 16S rRNA gene sequence similarity of 96.3 %, was Methanobacterium beijingense 8-2 T (Ma et al, 2005). Since its 16S rRNA gene sequence showed ,97 % similarity with the corresponding sequences of recognized species of the genus Methanobacterium, strain 17A1 T should be considered to represent a novel species (Stackebrandt & Goebel, 1994).At the time of writing, the genus Methanobacterium comprised 19 species with validly published names (Bonin & Boone, 2006;Kitamura et al, 2011;Krivushin et al, 2010;Ma et al, 2005;Mori & Harayama, 2011;Shcherbakova et al, 2011;Shlimon et al, 2004;Zhu et al, 2011). These species usually grow by reducing CO 2 with H 2 .…”

“…The 16S rRNA genes of the novel strain and cloned isolates from the sediments of the dystrophic Lake Grosse Fuchskuhle (Chan et al, 2002; GenBank accession number AF481340) and the meromictic Lake Kinneret (Nüsslein et al, 2001; AJ310872) were found to be similar (.97 % sequence similarity). The cultivated, recognized species that appeared most closely related to strain 17A1 T , with a 16S rRNA gene sequence similarity of 96.3 %, was Methanobacterium beijingense 8-2 T (Ma et al, 2005). Since its 16S rRNA gene sequence showed ,97 % similarity with the corresponding sequences of recognized species of the genus Methanobacterium, strain 17A1 T should be considered to represent a novel species (Stackebrandt & Goebel, 1994).…”

“…At the time of writing, the genus Methanobacterium comprised 19 species with validly published names (Bonin & Boone, 2006;Kitamura et al, 2011;Krivushin et al, 2010;Ma et al, 2005;Mori & Harayama, 2011;Shcherbakova et al, 2011;Shlimon et al, 2004;Zhu et al, 2011). These species usually grow by reducing CO 2 with H 2 .…”

“…Growth was stimulated by the addition of acetate (5 mM), vitamins (5 ml l 21 ), yeast extract (0.2 g l 21 ) or rumen fluid (5 ml l 21 ). Strain 17A1 T utilized H 2 /CO 2 and methanol (Ma et al, 2005); 3, Methanobacterium veterum MK4 T (Krivushin et al, 2010); 4, Methanobacterium formicicum MF T (Bryant & Boone, 1987); 5, Methanobacterium palustre F T (Zellner et al, 1988); 6, Methanobacterium espanolae GP9 T (Patel et al, 1990). 2, Negative; +, positive; ND, not determined; E, essential; NR, not required; S, stimulatory.…”

Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors

“…The dominant clones were most closely related to Methanobacterium beijingense (AY350742), Methanosae ta harundinacea (AY970347), and Methanosaeta concilii (M59146). M. beijingense, a methanogen recently isolated from an anaerobic digester, can utilize H 2 /CO 2 or formate to form methane, but not acetate (Ma et al 2005). In contrast, M. harundinacea, a microorganism isolated from a UASB reactor treating brewery wastewater (Ma et al 2006), and M. concilii, a methanogen commonly found in anaerobic reactors (Ekiel et al 1985;Patel and Sprott 1990), are obligate acetoclastic methanogens.…”