Methane-rich water alleviates NaCl toxicity during alfalfa seed germination

Zhu, Ke‐Xue; Cui, Weiti; Dai, Chen; Wu, Mingzhu; Zhang, Jing; Zhang, Yihua; Xie, Yanjie; Shen, Wenbiao

doi:10.1016/j.envexpbot.2015.11.013

Cited by 32 publications

(25 citation statements)

References 62 publications

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“…2). Thus, our study suggested that CH 4 production in plants can be induced by environmental stimuli, including osmotic stress and salinity23.…”

Section: Discussionmentioning

confidence: 66%

“…A beneficial role of CH 4 against salinity stress has been recently reported in alfalfa plants, which was mainly attributed to the induction of antioxidant defence23. To investigate the regulatory mechanism of CH 4 on redox status, the total activities of SOD, POD, and CAT were determined in PEG-stressed plants (Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, osmotic stress-induced ROS overproduction and corresponding oxidative reactions could result in the increases in lipid peroxidation and even the loss of biomass. For example, ROS overproduction is harmful to nucleic acids, proteins, lipids, and sugar if accumulated over a certain threshold in plants2223. In order to avoid the overproduction of ROS caused by osmotic stress, plants have evolved specific antioxidant systems to ensure a control of the cellular redox state24, and these include the non-enzymatic components (ascorbic acid, AsA; glutathione, GSH; etc), and the antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (POD), etc25.…”

mentioning

confidence: 99%

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Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Han

Duan

Wang

et al. 2017

Sci Rep

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Although aerobic methane (CH 4 ) release from plants leads to an intense scientific and public controversy in the recent years, the potential functions of endogenous CH 4 production in plants are still largely unknown. Here, we reported that polyethylene glycol (PEG)-induced osmotic stress significantly increased CH 4 production and soluble sugar contents in maize (Zea mays L.) root tissues. These enhancements were more pronounced in the drought stress-tolerant cultivar Zhengdan 958 (ZD958) than in the drought stress-sensitive cultivar Zhongjiangyu No.1 (ZJY1). Exogenously applied 0.65 mM CH 4 not only increased endogenous CH 4 production, but also decreased the contents of thiobarbituric acid reactive substances. PEG-induced water deficit symptoms, such as decreased biomass and relative water contents in both root and shoot tissues, were also alleviated. These beneficial responses paralleled the increases in the contents of soluble sugar and the reduced ascorbic acid (AsA), and the ratio of AsA/dehydroascorbate (DHA). Further comparison of transcript profiles of some key enzymes in sugar and AsA metabolism suggested that CH 4 might participate in sugar signaling, which in turn increased AsA production and recycling. Together, these results suggested that CH 4 might function as a gaseous molecule that enhances osmotic stress tolerance in maize by modulating sugar and AsA metabolism.Methane (CH 4 ) is the most abundant reduced organic compound in the atmosphere, and also the second important greenhouse gas after carbon dioxide 1,2 . It was previously considered as a degradation product of organic substance by microbes under anoxic conditions 3 . Keppler et al. 4 further reported a surprising discovery that terrestrial plants can produce CH 4 under aerobic conditions. Although much controversy and debate followed this original work, a number of researchers have attempted to provide alternate explanations of the aerobic CH 4 formation from plants using different scales of measurement 5 . In fact, the non-microbial CH 4 production from plants constitutes a significant fraction of the global CH 4 sources 2,6 . A comprehensive understanding of CH 4 in plants is that the living plants and fungi can not only emit CH 4 to the atmosphere 7 , but also produce CH 4 in plants in vivo 8 . Interestingly, this phenomenon was observed in the researches of nitric oxide 9 , carbon monoxide 10 , as well as hydrogen gas 11 . In some plant species, several chemical compounds, such as lignin, cellulose, leaf surface wax, ascorbic acid (AsA), the methyl groups of sulphur-containing amino acid methionine (Met), were suggested to be the potential precursors of CH 4 2,5,12,13 .

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“…2). Thus, our study suggested that CH 4 production in plants can be induced by environmental stimuli, including osmotic stress and salinity23.…”

Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Han

Duan

Wang

et al. 2017

Sci Rep

Self Cite

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“…Subsequently, the vial was heated at 70 ∘ C for 1 h to liberate CH 4 from plant tissues and allowed to cool at room temperature before the headspace gas (100 μL) was analyzed. CH 4 content was estimated using an Agilent 7820 model gas chromatograph (Agilent Technologies Inc., USA) according to Zhu et al 13 with minor modifications. The following conditions were used: Poropak column (1/8 inch, 8 foot); flame ionization detector; the oven, injector, and detector temperature were 80 ∘ C, 200 ∘ C, and 300 ∘ C respectively; hydrogen flow, 50 mL min −1 ; air flow, 400 mL min −1 ; with carrier gas flow (nitrogen), 25 mL min.…”

Section: Measurement Of Ch 4 Contentmentioning

confidence: 99%

“…For example, using methane-rich water (MRW), it has been proposed that CH 4 triggers cucumber adventitious root formation 12 and confers protection against salt and heavy metal stresses in alfalfa plants. 13,14 Wishkerman et al also found that CH 4 formation may occur in all eukaryotes as an integral part of the cellular response toward changes in the oxidative status. 15 This evidence shows that CH 4 can protect tissues against oxidative damage in medicine and recently in various abiotic stresses of plants.…”

Section: Introductionmentioning

confidence: 98%

Methane delays the senescence and browning in daylily buds by re‐established redox homeostasis

Liú

2017

J Sci Food Agric

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Making plant methane formation visible—Insights from application of ¹³C‐labeled dimethyl sulfoxide

Schroll

Lenhart

Greiner³

et al. 2022

Plant Enviro Interactions

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Methane (CH4) formation by vegetation has been studied intensively over the last 15 years. However, reported CH4 emissions vary by several orders of magnitude, thus making global estimates difficult. Moreover, the mechanism(s) for CH4 formation by plants is (are) largely unknown. Here, we introduce a new approach for making CH4 formation by plants clearly visible. By application of 13C‐labeled dimethyl sulfoxide (DMSO) onto the leaves of tobacco plants (Nicotiana tabacum) and Chinese silver grass (Miscanthus sinensis) the effect of light and dark conditions on CH4 formation of this pathway was examined by monitoring stable carbon isotope ratios of headspace CH4 (δ13C‐CH4 values). Both plant species showed increasing headspace δ13C‐CH4 values while exposed to light. Higher light intensities increased CH4 formation rates in N. tabacum but decreased rates for M. sinensis. In the dark no formation of CH4 could be detected for N. tabacum, while M. sinensis still produced ~50% of CH4 compared to that during light exposure. Our findings suggest that CH4 formation is clearly dependent on light conditions and plant species and thus indicate that DMSO is a potential precursor of vegetative CH4. The novel isotope approach has great potential to investigate, at high temporal resolution, physiological, and environmental factors that control pathway‐specific CH4 emissions from plants.

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Methane-rich water alleviates NaCl toxicity during alfalfa seed germination

Cited by 32 publications

References 62 publications

Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Methane delays the senescence and browning in daylily buds by re‐established redox homeostasis

Making plant methane formation visible—Insights from application of ¹³C‐labeled dimethyl sulfoxide

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Methane-rich water alleviates NaCl toxicity during alfalfa seed germination

Cited by 32 publications

References 62 publications

Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Methane delays the senescence and browning in daylily buds by re‐established redox homeostasis

Making plant methane formation visible—Insights from application of 13C‐labeled dimethyl sulfoxide

Contact Info

Product

Resources

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Making plant methane formation visible—Insights from application of ¹³C‐labeled dimethyl sulfoxide