Methane production in ruminants: its effect on the doubly labeled water method

Midwood, Andrew J.; Haggarty, Paul; McGaw, Brian A.; Robinson, J. J.

doi:10.1152/ajpregu.1989.257.6.r1488

Cited by 15 publications

(41 citation statements)

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“…To calculate the effect of methanogenesis on the DLW method it is necessary to know the rate of CH 4 production (rCH 4 ) and the stoichiometry of water H incorporation into CH 4 . Midwood et al (1989) have shown that each litre of CH 4 produced will cause rH 2 O to be overestimated by 1 . 052 g and rCO 2 to be underestimated by 0 .…”

Section: Resultsmentioning

confidence: 99%

“…629 litres. These authors suggested that rCH 4 may be calculated from an estimate of rCO 2 since the rCO 2 :rCH 4 ratio is relatively stable, falling between 10 and 20 for a wide range of animals fed at or above maintenance on very different diets with forage:concentrate ratios of 100:0 through to 20:80 (Midwood et al 1989). For example, Brockway & Maloiy (1967) measured an approximate rCO 2 :CH 4 ratio of 16 in red deer consuming chopped hay.…”

Section: Resultsmentioning

confidence: 99%

“…The doubly-labelled water (DLW) technique (Lifson & McClintock, 1966) is currently the most promising method of estimating energy expenditure in free-living animals. The DLW method has now been successfully validated in ruminant (Fancy et al 1986;Midwood et al 1994) and single-stomached (Haggarty et al 1994a) production animals and may therefore be used with confidence in a wide variety of metabolic states if appropriate corrections are made for the processes which are known to introduce errors into the technique (Midwood et al 1989;1993;Haggarty, 1991;Haggarty et al 1994a). In the present study, DLW was used to estimate the energy expenditure of adult non-pregnant red deer hinds in summer whilst on lowland pasture under typical farming conditions.…”

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confidence: 99%

“…This may occur during sequestration of 2 H into stable C-H bonds or by exchange of 2 H with labile positions on material which is subsequently exported from the body; faeces for example. The sequestration and exchange processes which have the potential to introduce substantial errors into the DLW method in production animals are: fat synthesis, CH 4 production and export of faecal DM (Midwood et al 1989;Haggarty, 1991;Haggarty et al 1994a). The magnitude of these processes must be estimated in order to make the necessary corrections to the isotope flux data (Haggarty, 1991).…”

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Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method

Haggarty¹,

Robinson²,

Ashton³

et al. 1998

Br J Nutr

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Energy expenditure was estimated using the doubly-labelled water (DLW) method in summer in five free-living adult, non-pregnant, non-lactating, red deer (Cervus elaphus) hinds (weight 107 . 3 (SE 0 . 9) kg; age 6 (SE 1) years) on lowland pasture under typical farming conditions. Climatic conditions were monitored throughout the experiment. Errors due to 2 H losses in CH 4 and faeces were calculated from previous estimates of stoichiometries. CH 4 production, fractionated water loss, urinary N and O 2 consumption were estimated using an iterative approach. The water flux (rH 2 O) in these animals consuming only fresh grass was 12 (SE 0 . 5) kg/d, the CO 2 production (rCO 2 ) was 1271 (SE 40) litres/d and the mean energy expenditure was 25 (SE 0 . 8) MJ/d. There were no significant differences in the isotope distribution spaces and flux rates, rH 2 O, rCO 2 or energy expenditure using the multi-point or two-point approaches to calculation. The DLWderived energy expenditure of 25 MJ/d is approximately 20 % higher than the recommended intake of 21 MJ/d for adult hinds kept outdoors (Adam, 1986) and, at 757 kJ/kg 0.75 per d, one third higher than the value of 570 kJ/kg 0.75 per d for stags penned indoors (Key et al. 1984). Energy expenditure: Doubly-labelled water: Red deerDownloaded from https://www.cambridge.org/core. IP address: 44.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method

Haggarty¹,

Robinson²,

Ashton³

et al. 1998

Br J Nutr

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“…Para a estimativa de produção de metano (Midwood et al, 1989(Midwood et al, , 1993 O calculo do N microbiano é apresentado na equação abaixo:…”

Section: Coleta De Urina E Produção Microbianaunclassified

Desempenho, metabolismo e emissão de metano de bovinos Nelore em terminação recebendo óleos funcionais em substituição ou combinação com monensina sódica na dieta

Chagas¹

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Isotope partitioning between cow milk and farm water: A tool for verification of milk provenance

Boito

Iacumin

Rossi

et al. 2021

Rapid Comm Mass Spectrometry

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The oxygen and hydrogen isotope composition of the water component of the milk from nine Italian dairy farms were studied together with the farm water for one year. The aim was to verify the importance of farm water and seasonal temperature variation on milk isotope values and propose mathematical relations as new tools to identify the milk origin.METHODS: Milk was centrifuged to separate the solids and then distilled under a vacuum to separate water. δ( 18 O/ 16 O) and δ( 2 H/ 1 H) analyses of the water molecules were carried out using a water equilibrator online with a mass spectrometer. For oxygen and hydrogen isotope determination, water was equilibrated with pure CO2 for 7.5 hours and with pure H2 for 5 hours, respectively. The isotope ratio value is indicated with  (expressed in the VSMOW/SLAP scale) as defined by IUPAC. RESULTS:The average annual isotope value of milk at the different cattle sheds is mostly related to the farm water suggesting that the drinking water is the most important factor influencing the isotopic values of the milk water. The milk/water fractionation factor correlates with the milking time and, thus, the seasonal temperature is best described by a 4 th order This article is protected by copyright. All rights reserved. polynomial regression line. A two level-check model was used to verify the milking provenance. CONCLUSIONS:This study shows that it is essential to analyze both milk and farm water to indicate provenance. A two-step verification tool, based on the difference between the measured and calculated δ( 18 O/ 16 O)M values, and the difference between the calculated and estimated milk-water fractionation factor, allowed the source determination of milk. Both conditions must be met if the milk is considered to be from the Parmigiano-Reggiano production region. Although this approach was developed for this region, it can easily be tested and adapted to other dairy production areas.

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Methane production in ruminants: its effect on the doubly labeled water method

Cited by 15 publications

References 0 publications

Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method

Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method

Desempenho, metabolismo e emissão de metano de bovinos Nelore em terminação recebendo óleos funcionais em substituição ou combinação com monensina sódica na dieta

Isotope partitioning between cow milk and farm water: A tool for verification of milk provenance

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