Methane Production in a West Siberian Eutrophic Fen Is Much Higher than Carbon Dioxide Production: Incubation of Peat Samples, Stoichiometry, Stable Isotope Dynamics, Modeling

Lokshina, L. Ya.; Вавилин, В.А.; Litti, Yu. V.; Glagolev, M. V.; Сабреков, А. Ф.; Kotsyurbenko, O. R.; Kozlova, M. A.

doi:10.1134/s0097807819070133

Cited by 19 publications

(7 citation statements)

References 37 publications

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“…The oxygen and carbon dioxide concentrations in the headspace of the bottles were determined by gas chromatograph Crystal 5000.2 (Chromatec, Yoshkar-Ola, Russia) as described earlier [ 55 ]. BOD 5 was determined by the OxiTop respirometric BOD measuring system (WTW, Oberbayern, Germany) according to manufacturer’s recommendations.…”

Section: Methodsmentioning

confidence: 99%

Didecyldimethylammonium Chloride- and Polyhexamethylene Guanidine-Resistant Bacteria Isolated from Fecal Sludge and Their Potential Use in Biological Products for the Detoxification of Biocide-Contaminated Wastewater Prior to Conventional Biological Treatment

Лойко

Kanunnikov²,

Serdyukov

et al. 2022

Biology

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Toxic shock caused by the discharge of biocide-contaminated fecal sludge (FS) from chemical toilets to conventional wastewater treatment plants (WWTP) can be a major problem in activated sludge operation. It is necessary to develop new environmental approaches to mitigate the toxicity of biocides in order to avoid degrading the performance of WWTP. “Latrina”, a chemical toilet additive containing didecyldimethylammonium chloride and polyhexamethylene guanidine, is widely used in environmentally safe toilet complexes (ESTC) on Russian railway trains to deodorize FS and control microbial activity. In this work, seven biocide-resistant bacterial strains were isolated and identified from the FS of ESTC. The values of the minimum inhibitory and bactericidal concentrations of biocides for the isolated strains were 4.5–10 times higher than for the collection microorganisms. The bacterium Alcaligenes faecalis DOS7 was found to be particularly resistant to “Latrina”, the minimum inhibitory concentration of which was almost 30 times higher than recommended for ESTC. Biological products based on isolated bacterial strains proved to be effective for FS biodegradation under both aerobic and anaerobic conditions. The results of the biochemical oxygen demand test and the newly developed disk-diffusion bioassay confirmed that isolated strains contribute to reducing toxicity of biocidal agents in FS. Hyper-resistance, non-pathogenicity, and potential plant growth-promoting ability make A. faecalis DOS7 promising for use in various biological products for wastewater treatment and bioremediation of soils contaminated with biocides, as well as in agriculture to increase plant productivity.

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Section: Methodsmentioning

confidence: 99%

Didecyldimethylammonium Chloride- and Polyhexamethylene Guanidine-Resistant Bacteria Isolated from Fecal Sludge and Their Potential Use in Biological Products for the Detoxification of Biocide-Contaminated Wastewater Prior to Conventional Biological Treatment

Лойко

Kanunnikov²,

Serdyukov

et al. 2022

Biology

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“…δD(СН 4 ) mod,CR is calculated according to Whiticar (1999) The contribution of the CO 2 reduction pathway could be underestimated in our analysis since the models from Douglas et al (2021) and Waldron et al (1999) may not exclusively represent the acetate fermentation pathway. These models are based on in situ data from freshwater wetlands where methane production via the CO 2 reduction pathway can also play a significant role (Conrad, 2020;Kotsyurbenko et al, 2004;Lokshina et al, 2019). However, since we do not have samples with δD(СН 4 ) values outside the range predicted by these models (as seen in Figure S2), their accuracy for our data is considered acceptable.…”

Section: Methanogenic Pathway Identificationmentioning

confidence: 99%

Methane in West Siberia terrestrial seeps: Origin, transport, and metabolic pathways of production

Сабреков

Terentieva

McDermid

et al. 2023

Global Change Biology

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The expansive plains of West Siberia contain globally significant carbon stocks, with Earth's most extensive peatland complex overlying the world's largest‐known hydrocarbon basin. Numerous terrestrial methane seeps have recently been discovered on this landscape, located along the floodplains of the Ob and Irtysh Rivers in hotspots covering more than 2500 km2. We articulated three hypotheses to explain the origin and migration pathways of methane within these seeps: (H1) uplift of Cretaceous‐aged methane from deep petroleum reservoirs along faults and fractures, (H2) release of Oligocene‐aged methane capped or trapped by degrading permafrost, and (H3) horizontal migration of Holocene‐aged methane from surrounding peatlands. We tested these hypotheses using a range of geochemical tools on gas and water samples extracted from seeps, peatlands, and aquifers across the 120,000 km2 study area. Seep‐gas composition, radiocarbon age, and stable isotope fingerprints favor the peatland hypothesis of seep‐methane origin (H3). Organic matter in raised bogs is the primary source of seep methane, but observed variability in stable isotope composition and concentration suggest production in two divergent biogeochemical settings that support distinct metabolic pathways of methanogenesis. Comparison of these parameters in raised bogs and seeps indicates that the first is bogs, via CO2 reduction methanogenesis. The second setting is likely groundwater, where dissolved organic carbon from bogs is degraded via chemolithotrophic acetogenesis followed by acetate fermentation methanogenesis. Our findings highlight the importance of methane lateral migration in West Siberia's bog‐dominated landscapes via intimate groundwater connections. The same phenomenon could occur in similar landscapes across the boreal‐taiga biome, thereby making groundwater‐fed rivers and springs potent methane sources.

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“…It had been previously thought that, under stable anaerobic CH4-producing conditions, the lowest possible ratio of CO2:CH4 production is 1:1 (Conrad, 1999). However, some recent studies from Arctic environments have reported CO2:CH4 ratios of less than 1, indicating more CH4 production than CO2 production (Heslop et al, 2019b;Knoblauch et al, 2018;Lokshina et al, 2019;Walter Anthony et al, 2014). At a eutrophic fen site in Siberia, the higher CH4 production than CO2 production was attributed to proportions of CH4 produced through acetoclastic (Equation 2) versus hydrogenotrophic (Equation 1) methanogenesis; adding an inhibitor to change the major pathway from acetoclastic to hydrogenotrophic methanogenesis increased CO2:CH4 ratios from 0.3-0.4 to 0.7-0.9 (Lokshina et al, 2019).…”

Section: Lake Emissions Lake Sedimentmentioning

confidence: 99%

A synthesis of methane dynamics in thermokarst lake environments

Heslop

Anthony

Winkel

et al. 2020

Earth-Science Reviews

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Greenhouse gas emissions from physical permafrost thaw disturbance and subsidence, including the formation and expansion of thermokarst (thaw) lakes, may double the magnitude of the permafrost carbon feedback this century. These processes are not accounted for in current global climate models. Thermokarst lakes, in particular, have been shown to be hotspots for emissions of methane (CH4), a potent greenhouse gas with 32 times more global warming potential than carbon dioxide (CO2) over a 100-year timescale. Here, we synthesize several studies examining CH4 dynamics in a representative first-generation thermokarst lake (Vault Lake, informal name) to show that CH4 production and oxidation potentials vary with depth in thawed sediments beneath the lake. This variation leads to depth-dependent differences in both in situ dissolved CO2:CH4 ratios and net CH4 production responses to additional warming. Comparing CH4 production, oxidation, and flux values from studies at Vault Lake suggests up to 99% of produced CH4 is oxidized and/or periodically entrapped before entering the atmosphere. We summarize these findings in the context of CH4 literature from thermokarst lakes and identify future research directions for incorporating thermokarst lake CH4 dynamics into estimates of the permafrost carbon feedback.

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Methane Production in a West Siberian Eutrophic Fen Is Much Higher than Carbon Dioxide Production: Incubation of Peat Samples, Stoichiometry, Stable Isotope Dynamics, Modeling

Cited by 19 publications

References 37 publications

Didecyldimethylammonium Chloride- and Polyhexamethylene Guanidine-Resistant Bacteria Isolated from Fecal Sludge and Their Potential Use in Biological Products for the Detoxification of Biocide-Contaminated Wastewater Prior to Conventional Biological Treatment

Didecyldimethylammonium Chloride- and Polyhexamethylene Guanidine-Resistant Bacteria Isolated from Fecal Sludge and Their Potential Use in Biological Products for the Detoxification of Biocide-Contaminated Wastewater Prior to Conventional Biological Treatment

Methane in West Siberia terrestrial seeps: Origin, transport, and metabolic pathways of production

A synthesis of methane dynamics in thermokarst lake environments

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