Methane inhibition by
            <i>Asparagopsis taxiformis</i>
            with rumen fluid collected from ventral and central location – a pilot study

Álvarez, Clementina; Andersen, Thea Os; Eikanger, Katrine Sømliøy; Hamfjord, Ida Wøyen; Niu, Puchun; Weiby, Kim Viggo; Årvik, Linda; Dörsch, Peter; Hagen, Live Heldal; Pope, Phillip B.; Forberg, Dag Kristoffer; Hustoft, Hanne Kolsrud; Schwarm, Angela; Kidane, Alemayehu

doi:10.1080/09064702.2022.2152196

Cited by 1 publication

(1 citation statement)

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“…The samples were incubated in 100 mL of buffered [29] rumen fluid (67 mL buffer solution and 33 mL rumen fluid) for 48 h using 250 mL glass bottles. For this, the rumen fluid was collected about 3 h post-morning feeding from three rumen cannulated cows fed according to the Norfor [30] feeding standard with a diet composition as recently described in Alvarez et al [31]. The rumen fluid was collected into two pre-warmed thermos flasks and filtered through a nylon cloth (SEFAR NITEX, Sefar AG, Heiden, Switzerland) with a pore size of 200 µm into glass bottles maintained in a 39 • C water bath.…”

Section: In Vitro Gas Productionmentioning

confidence: 99%

In Vitro Rumen Fermentation Characteristics, Estimated Utilizable Crude Protein and Metabolizable Energy Values of Grass Silages, Concentrate Feeds and Their Mixtures

Muqier,

Eknæs,

Prestløkken

et al. 2023

Animals

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Four formulations of concentrate feeds, three contrasting qualities of grass silages, and mixtures of the silages (55%) and concentrates (45%, dry weight) were tested for in vitro fermentation kinetics, in vitro dry matter degradation (IVDMD), utilizable crude protein (uCP), and metabolizable energy (ME) values. The concentrates were pelleted control concentrate for dairy cows (CONT-P); pelleted alkaline concentrate with ammoniated cereal grains (ALKA-P); mash form concentrate with ALKA-P main ingredients but with feed-grade urea and barley replacing ammoniated cereal grain (UREA-M); and mash form of ALKA-P ingredients prior to alkalization (ALKA-M). The grass silages were early cut, late cut, and a mixture (1:1) of early and late cut. The objectives were to test if the feeds differed in the tested parameters within each feed category and assess the modulatory effect of concentrate feeds on the grass silage fermentation characteristics in the mixed diets. No interaction effects of the concentrate feeds by silage quality were observed for the tested parameters in the mixed diets. For concentrates, the pelleted diets were higher (p < 0.05) in IVDMD and molar proportion of propionate but lower in butyrate. The ALKA-P produced the highest estimated uCP (p < 0.01). For silages, uCP, ME, total short-chain fatty acids (VFAs), and molar proportions of propionate and branched-chain VFAs decreased (p < 0.05) with increasing stage of maturity. In conclusion, the ALKA-P could match the CONT-P in uCP and ME values and fermentation characteristics. Results for silages and their mixtures with concentrates highlight the importance of silage quality in dietary energy and protein supply for ruminants.

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Section: In Vitro Gas Productionmentioning

confidence: 99%