Methacrylated pullulan/polyethylene (glycol) diacrylate composite hydrogel for cartilage tissue engineering

Qin, Xiaoping; He, Rui; Chen, Hao; Fu, Dejie; Peng, Yang; Meng, Shuo; Chen, Cheng; Yang, Liu

doi:10.1080/09205063.2021.1899888

Cited by 25 publications

(9 citation statements)

References 35 publications

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“…The results showed that the addition of PEGDA reduced its degradation rate, the residual mass increased from 63.9% to 86.8%, and the compression modulus increased by 13 times to 1.17 MPa. 27 PEGDA is a kind of synthetic hydrogel, which can be cross-linked by free radical polymerization initiated by ultraviolet light or redox reaction. It has good printability and mechanical properties, 28 but its biocompatibility is not as good as natural hydrogels such as gelatin and sodium alginate.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair

et al. 2021

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Natural cartilage tissue has excellent mechanical properties and has certain cellular components. At this stage, it is a great challenge to produce cartilage scaffolds with excellent mechanical properties, biocompatibility, and biodegradability. Hydrogels are commonly used in tissue engineering because of their excellent biocompatibility; however, the mechanical properties of commonly used hydrogels are difficult to meet the requirements of making cartilage scaffolds. The mechanical properties of high concentration polyethylene glycol diacrylate (PEGDA) hydrogel are similar to those of natural cartilage, but its biocompatibility is poor. Low concentration hydrogel has better biocompatibility, but its mechanical properties are poor. In this study, two different hydrogels were combined to produce cartilage scaffolds with good mechanical properties and strong biocompatibility. First, the PEGDA grid scaffold was printed with light curing 3D printing technology, and then the low concentration GelMA/Alginate hydrogel with chondral cells was filled into the PEGDA grid scaffold. After a series of cell experiments, the filling hydrogel with the best biocompatibility was screened out, and finally the filled hydrogel with cells and excellent biocompatibility was obtained. Cartilage tissue engineering scaffolds with certain mechanical properties were found to have a tendency of cartilage formation in in vitro culture. Compared with the scaffold obtained by using a single hydrogel, this molding method can produce a tissue engineering scaffold with excellent mechanical properties on the premise of ensuring biocompatibility, which has a certain potential application value in the field of cartilage tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair

et al. 2021

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“…In the direct contact tests, the samples result in greater than 70% cell viability, with the exception of 0.5% PI samples. Such discrepancies between eluate and direct contact tests have been reported, and are commonly explained by the fact that, for the evaluation of differentiation and proliferation of the cells, their attachment to the tested material is crucial [ 21 , 50 , 51 , 52 ]. Hence, the low attachment of cells leads to low overall cell counts.…”

Section: Discussionmentioning

confidence: 99%

“…Different combinations of PEGDA-based materials have enabled the development of numerous devices for use in diverse biomedical applications. Examples of such implementations have already been reported: PEGDA in composition with gelatin methacrylate as injectable hydrogels for periodontal treatments [ 15 ] PEGDA hydrogel microneedles patches as a drug delivery for the treatment of skin diseases [ 16 ] PEGDA as an in vitro 3D cancer model for different types of cancer cells [ 17 ] Compositions of PEGDA hydrogels with different molecular masses for cartilage repair [ 18 ] PEGDA/chitosan films as a wound-dressing material [ 19 ] 3D-printed PEGDA anti-inflammatory scaffolds for regeneration of osteochondral defects [ 20 ] Composite hydrogels for cartilage tissue engineering [ 21 ] Lobule-like hepatocyte-laden 3D constructs in combination with gelatin methacrylate [ 22 ] 3D bioinks for cardiac tissue engineering [ 23 ] …”

Section: Introductionmentioning

confidence: 99%

Thermal, Mechanical and Biocompatibility Analyses of Photochemically Polymerized PEGDA250 for Photopolymerization-Based Manufacturing Processes

Rekowska¹,

Huling²,

Brietzke³

et al. 2022

Pharmaceutics

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Novel fabrication techniques based on photopolymerization enable the preparation of complex multi-material constructs for biomedical applications. This requires an understanding of the influence of the used reaction components on the properties of the generated copolymers. The identification of fundamental characteristics of these copolymers is necessary to evaluate their potential for biomaterial applications. Additionally, knowledge of the properties of the starting materials enables subsequent tailoring of the biomaterials to meet individual implantation needs. In our study, we have analyzed the biological, chemical, mechanical and thermal properties of photopolymerized poly(ethyleneglycol) diacrylate (PEGDA) and specific copolymers with different photoinitiator (PI) concentrations before and after applying a post treatment washing process. As comonomers, 1,3-butanediol diacrylate, pentaerythritol triacrylate and pentaerythritol tetraacrylate were used. The in vitro studies confirm the biocompatibility of all investigated copolymers. Uniaxial tensile tests show significantly lower tensile strength (82% decrease) and elongation at break (76% decrease) values for washed samples. Altered tensile strength is also observed for different PI concentrations: on average, 6.2 MPa for 1.25% PI and 3.1 MPa for 0.5% PI. The addition of comonomers lowers elongation at break on average by 45%. Moreover, our observations show glass transition temperatures (Tg) ranging from 27 °C to 56 °C, which significantly increase with higher comonomer content. These results confirm the ability to generate biocompatible PEGDA copolymers with specific thermal and mechanical properties. These can be considered as resins for various additive manufacturing-based applications to obtain personalized medical devices, such as drug delivery systems (DDS). Therefore, our study has advanced the understanding of PEGDA multi-materials and will contribute to the future development of tools ensuring safe and effective individual therapy for patients.

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“…For example, pullulan could be modified with MA, lithium phenyl (2,4,6-trimethylbenzoyl) phosphinate (LAP), and PEG diacrylate (PEGDA) to form a hybrid hydrogel system, which has good mechanical properties and slow degradation rate for cartilage repair and regeneration. 94 In addition, gelatin, HA and MA are used together in a composite hydrogel to make scaffolds, which regenerated mature cartilage with typical lacunae structure and cartilage-specific ECM successfully when combined with chondrocytes; the hydrogel provided a novel natural 3D scaffold with satisfactory outer shape, pore structure, mechanical strength, degradation rate, and weak immunogenicity for cartilage regeneration. 95 …”

Section: Articulate Cartilage Defects Therapeutic Strategiesmentioning

confidence: 99%

Regeneration of articular cartilage defects: Therapeutic strategies and perspectives

Guo

Ling

et al. 2023

J Tissue Eng

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Articular cartilage (AC), a bone-to-bone protective device made of up to 80% water and populated by only one cell type (i.e. chondrocyte), has limited capacity for regeneration and self-repair after being damaged because of its low cell density, alymphatic and avascular nature. Resulting repair of cartilage defects, such as osteoarthritis (OA), is highly challenging in clinical treatment. Fortunately, the development of tissue engineering provides a promising method for growing cells in cartilage regeneration and repair by using hydrogels or the porous scaffolds. In this paper, we review the therapeutic strategies for AC defects, including current treatment methods, engineering/regenerative strategies, recent advances in biomaterials, and present emphasize on the perspectives of gene regulation and therapy of noncoding RNAs (ncRNAs), such as circular RNA (circRNA) and microRNA (miRNA).

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Methacrylated pullulan/polyethylene (glycol) diacrylate composite hydrogel for cartilage tissue engineering

Cited by 25 publications

References 35 publications

Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair

Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair

Thermal, Mechanical and Biocompatibility Analyses of Photochemically Polymerized PEGDA250 for Photopolymerization-Based Manufacturing Processes

Regeneration of articular cartilage defects: Therapeutic strategies and perspectives

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