Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

Kessler, Lukas; Gehrke, Sandra; Winnefeld, Marc; Huber, Birgit; Hoch, Eva; Walter, Torsten; Wyrwa, Ralf; Schnabelrauch, Matthias; Schmidt, Malte; Kückelhaus, Maximilian; Lehnhardt, Marcus; Hirsch, Tobias; Jacobsen, Frank

doi:10.1177/2041731417744157

Cited by 55 publications

(29 citation statements)

References 63 publications

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“…Human [88] ASCs were able to remain viable and differentiate into adipocytes in a 4-week time span. This approach was limited by the lack of vascularization [88].…”

Section: Methacrylated Gelatin and Hyaluronan Ascsmentioning

confidence: 99%

Adipose Tissue Fibrosis: Mechanisms, Models, and Importance

DeBari

Abbott

2020

IJMS

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Increases in adipocyte volume and tissue mass due to obesity can result in inflammation, further dysregulation in adipose tissue function, and eventually adipose tissue fibrosis. Like other fibrotic diseases, adipose tissue fibrosis is the accumulation and increased production of extracellular matrix (ECM) proteins. Adipose tissue fibrosis has been linked to decreased insulin sensitivity, poor bariatric surgery outcomes, and difficulty in weight loss. With the rising rates of obesity, it is important to create accurate models for adipose tissue fibrosis to gain mechanistic insights and develop targeted treatments. This article discusses recent research in modeling adipose tissue fibrosis using in vivo and in vitro (2D and 3D) methods with considerations for biomaterial selections. Additionally, this article outlines the importance of adipose tissue in treating other fibrotic diseases and methods used to detect and characterize adipose tissue fibrosis.

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“…Human [88] ASCs were able to remain viable and differentiate into adipocytes in a 4-week time span. This approach was limited by the lack of vascularization [88].…”

Section: Methacrylated Gelatin and Hyaluronan Ascsmentioning

confidence: 99%

Adipose Tissue Fibrosis: Mechanisms, Models, and Importance

DeBari

Abbott

2020

IJMS

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“…), gelatin presents a high biocompatibility, making it a great candidate to be used in the design of hydrogel-based implantable biomaterials. Indeed, as biomaterials for tissue engineering, a wide diversity of gelatin-based structures have been described in literature 17 including nano-and microspheres 18,19 or particles, 3D scaffolds [20][21][22][23][24] , electrospun nanofibers [25][26][27] , cryogel scaffolds 28 , composite materials [29][30][31] and in situ gelling formulations. This reverts in a broad range of applications going from drug and growth factors delivery 18,19,30 to tissue repair and regeneration for ocular 23 , bone 30 , skeletal muscle 32 or soft tissue 24,31 engineering.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Generation of Reactive Species by Cold Plasma in Gelatin Solutions for Selective Cancer Cell Death

Labay

Roldán

Tampieri

et al. 2020

ACS Appl. Mater. Interfaces

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Atmospheric pressure plasma jets generate reactive oxygen and nitrogen species (RONS) in liquids and biological media, which find application in the new area of plasma medicine. These plasma-treated liquids demonstrated recently to possess selective properties on killing cancer cells and attract attention towards new plasma-based cancer therapies. These allow for local delivery by injection in the tumor but can be quickly washed away by body fluids. By confining these RONS in a suitable biocompatible delivery system, great perspectives can be opened in the design of novel biomaterials aimed for cancer therapies. Gelatin solutions are evaluated here to store RONS generated by atmospheric pressure plasma jets, and their release properties are evaluated. The concentration of RONS was studied in 2% gelatin as a function of different plasma parameters (treatment time, nozzle distance and gas flow) with two different plasma jets. Much higher production of reactive species (H 2 O 2 and NO 2-) was revealed in the polymer solution than in water after plasma treatment. The amount of RONS generated in gelatin is greatly improved with respect to water, with concentrations of H 2 O 2 and NO 2between 2 and 12 times higher for the longest plasma treatments. Plasma-treated gelatin exhibited the release of these RONS to a liquid media, which induced an effective killing of bone cancer cells. Indeed, in vitro studies on Sarcoma Osteogenic (SaOS-2) cell line exposed to plasma-treated gelatin lead to timedependent increasing cytotoxicity with the longer plasma treatment time of gelatin. While SaOS-2 cell viability decreased down to 12%-23% after 72 hours for cells exposed to 3-min treated gelatin, viability of healthy cells (hMSC) was preserved (around 90%), establishing the selectivity of the plasma-treated gelatin on cancer cells. This sets the basis for designing improved hydrogels with high capacity to deliver RONS locally to tumors.

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“…Both HA and gelatin have been widely used as adipose tissue engineering biomaterials [24,61,62]. HA is also commonly used as a dermal and adipose filler to improve appearance and to correct deformities and loss of volume [63,64].…”

Section: Discussionmentioning

confidence: 99%

Effects of tunable, 3D-bioprinted hydrogels on human brown adipocyte behavior and metabolic function

Kuss

Kim

et al. 2018

Acta Biomaterialia

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Obesity and its related health complications cause billions of dollars in healthcare costs annually in the United States, and there are yet to be safe and long-lasting anti-obesity approaches. Using brown adipose tissue (BAT) is a promising approach, as it uses fats for energy expenditure. However, the effect of the microenvironment on human thermogenic brown adipogenesis and how to generate clinically relevant sized and functioning BAT are still unknown. In our current study, we evaluated the effects of endothelial growth medium exposure on brown adipogenesis of human brown adipose progenitors (BAP). We found that pre-exposing BAP to angiogenic factors promoted brown adipogenic differentiation and metabolic activity. We further 3D bioprinted brown and white adipose progenitors within hydrogel-based bioink with controllable physicochemical properties and evaluated the cell responses in 3D bioprinted environments. We used soft, stiff, and stiff-porous constructs to encapsulate the cells. All three types had high cell viability and allowed for varying levels of function for both white and brown adipocytes. We found that the soft hydrogel constructs promoted white adipogenesis, while the stiff-porous hydrogel constructs improved both white and brown adipogenesis and were the optimal condition for promoting brown adipogenesis. Consistently, stiff-porous hydrogel constructs showed higher metabolic activities than stiff hydrogel constructs, as assessed by 2-deoxy glucose uptake (2-DOG) and oxygen consumption rate (OCR). These findings show that the physicochemical environments affect the brown adipogenesis and metabolic function, and further tuning will be able to optimize their functions. Our results also demonstrate that 3D bioprinting of brown adipose tissues with clinically relevant size and metabolic activity has the potential to be a viable option in the treatment of obesity and type 2 diabetes.

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Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

Cited by 55 publications

References 63 publications

Adipose Tissue Fibrosis: Mechanisms, Models, and Importance

Adipose Tissue Fibrosis: Mechanisms, Models, and Importance

Enhanced Generation of Reactive Species by Cold Plasma in Gelatin Solutions for Selective Cancer Cell Death

Effects of tunable, 3D-bioprinted hydrogels on human brown adipocyte behavior and metabolic function

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