Methacrylate monolithic capillary columns for gradient peptide separations

Pruim, Peter; Öhman, Marcus; Huo, Yuli; Schoenmakers, Peter J.; Kok, Wim Th.

doi:10.1016/j.chroma.2008.08.069

Cited by 17 publications

(9 citation statements)

References 50 publications

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“…Making the gradient even more shallow apparently does not help. Similar findings were reported in previous research, where it became clear that the performance of methacrylate monoliths decreases at shallower gradients and so fast and steep gradients are advisable [30].…”

Section: Peptide Separationssupporting

confidence: 86%

“…From the plots it is apparent that especially on the BMA monolith early eluting compounds migrate with a relatively high average retention factor. In previous research in our laboratory it has been shown that the efficiency of methacrylate stationary phases for peptides and other small molecules strongly decreases with increasing (isocratic and gradient) retention factor [29,30]. Elution at a high retention factor over a part of the column length will therefore give rise to larger contribution to the final plate height.…”

Section: Peak Broadeningmentioning

confidence: 95%

“…This has resulted in a wide range of available materials with different monomers incorporated in the polymer to perform various modes of liquid chromatography [19][20][21][22][23][24][25] and in an increased understanding of the factors determining the analytical performance [26][27][28][29][30]. In a fused-silica chip pressure-driven reversed phase separations of peptides and proteins using a stearyl acrylate monolith have been performed in the isocratic mode by Reichmuth et al [31].…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Methacrylate monolithic stationary phases for gradient elution separations in microfluidic devices

Pruim¹,

Öhman²,

Schoenmakers³

et al. 2011

Journal of Chromatography A

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Section: Peptide Separationssupporting

confidence: 86%

Section: Peak Broadeningmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Methacrylate monolithic stationary phases for gradient elution separations in microfluidic devices

Pruim¹,

Öhman²,

Schoenmakers³

et al. 2011

Journal of Chromatography A

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“…Organic polymer-based monolithic columns have been successfully used in capillary electrochromatography (CEC) [1][2][3][4][5][6][7][8]. Polymer-based monoliths are attractive because there are a wide variety of different monomers available and can be used within a broad pH-rage where they are chemically stable.…”

Section: Introductionmentioning

confidence: 99%

Adamantyl-functionalized polymer monolith for capillary electrochromatography

Ohyama

Fukahori

Nakashima

et al. 2010

Journal of Chromatography A

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An adamantyl (ADM)-functionalized monolithic stationary phase was newly synthesized by a single-step copolymerization of 1-adamantyl-(-trifluoromethyl) acry--late, ethylene dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in order to prevent the peak tailing of basic solutes in capillary electrochromatography and was compared with butyl methacrylate (BMA)-based one. The ADM structure shields the negatively charged groups on the surface of monolith from basic solutes, resulting in better peak shapes than BMA-based monolithic stationary phase. As the monomers ratio decreased, the monolithic column had lower retention and higher column efficiency which was likely due to lower phase ratio and smaller globule size of monolith, respectively.The ADM-functionalized monolithic columns exhibited a good repeatability and reproducibility of column preparation with relative standard deviation values below 9% in the studied chromatographic parameters.3

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“…The ease of preparation and availability of various precursors also contributes to the popularity of monoliths [9]. A greater chromatography efficiency is shown by the monolithic columns for the separation of macromolecules such as nucleotides, oligonucleotides [10], peptides [11] and proteins [12][13][14][15]. Therefore, the monolithic colums have been applied in environmental, pharmaceutical, and genomic applications [16].…”

Section: Introductionmentioning

confidence: 99%

Separation of Inorganic Anions Using Methacrylate-Based Monolithic Column Modified with Trimethylamine in Ion Chromatography Capillary System

Mairizki¹,

Rahmah²,

nbsp³

et al. 2013

AJAC

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Methacrylate-based monolithic column was prepared in fused-silica capillary (80  0.32 mm i.d.) by in situ polymerizetion reaction using glycidyl methacrylate as monomer; ethylene dimethacrylate as crosslinker; 1-propanol, 1,4-butanediol, and water as porogenic solvents. The monolith matrix was modified with trimethylamine to create strong anion exchanger via ring opening reaction of epoxy groups. The morphology of the monolithic column was studied by using Scanning Electron Microscope (SEM). This column had good mechanical stability and permeability. The effects of various mobile phases for separation of inorganic anions were investigated. Iodate, bromate, nitrite, bromide, and nitrate were separated within 11 min using 100 mM potassium chloride as mobile phase and detected at 210 nm. This method showed good precision of retention time, acceptable linearity and good sensitivity. Under the optimum condition, the RSD of the retention time was in the range of 1.09% -1.75% (n = 6). The calibration curve showed linear relationships between the peak area and the concentration. The limits of detection (LOD) and the limits of quantitation (LOQ) were between 0.08 -0.18 mM and 0.26 -0.61 mM, respectively. This method was applied to the determination of inorganic anions in tap water and ground water samples.

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Methacrylate monolithic capillary columns for gradient peptide separations

Cited by 17 publications

References 50 publications

Methacrylate monolithic stationary phases for gradient elution separations in microfluidic devices

Methacrylate monolithic stationary phases for gradient elution separations in microfluidic devices

Adamantyl-functionalized polymer monolith for capillary electrochromatography

Separation of Inorganic Anions Using Methacrylate-Based Monolithic Column Modified with Trimethylamine in Ion Chromatography Capillary System

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