Methacholine-induced airway reactivity of inbred rats

Wang, C. G.; Dimaria, G.; Bates, Jason H. T.; Rd, Guttmann; Martin, James G.

doi:10.1152/jappl.1986.61.6.2180

Cited by 9 publications

(5 citation statements)

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“…Importantly, the results of serial cholinergic challenges were stable and similar among nonsensitized animals. Despite there being small intrastrain variability in AR to MCh challenge consistent with other reports (24,28), no evidence of tolerance or potentiation of cholinergic RL and Cdyn responsiveness was present with repeated measurements for as long Values are means Ϯ SE; n ϭ 8 mice/group. Leucocytes were recovered from BAL fluid according to Table 1.…”

Section: Discussionsupporting

confidence: 88%

Repetitive measurements of pulmonary mechanics to inhaled cholinergic challenge in spontaneously breathing mice

Glaab

Mitzner

Braun

et al. 2004

Journal of Applied Physiology

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Repetitive measurements of pulmonary mechanics to inhaled cholinergic challenge in spontaneously breathing mice. J Appl Physiol 97: 1104 -1111, 2004. First published April 30, 2004 10.1152/japplphysiol.01182.2003.-Precise and repeatable measurements of pulmonary function in intact mice are becoming increasingly important for experimental investigations on various respiratory disorders including asthma. Here, we present validation of a novel in vivo method that, for the first time, combines direct and repetitive recordings of standard pulmonary mechanics with cholinergic aerosol challenges in anesthetized, orotracheally intubated, spontaneously breathing mice. We demonstrate that, in several groups of nonsensitized BALB/c mice, dose-related increases in pulmonary resistance and dynamic compliance to aerosolized methacholine are reproducible over short and extended intervals without causing detectable cytological alterations in the bronchoalveolar lavage or relevant histological changes in the proximal trachea and larynx regardless of the number of orotracheal intubations. Moreover, as further validation, we confirm that allergic mice, sensitized and challenged with Aspergillus fumigatus, were significantly more responsive to cholinergic challenge (P Ͻ 0.01) and exhibited marked eosinophilia and lymphocytosis in bronchoalveolar lavage fluids as well as significant pathological alterations in laryngotracheal histology compared with nonsensitized mice. We suggest that this approach will provide useful and necessary information on pulmonary mechanics in studies of various respiratory disorders in mice, including experimental models of asthma and chronic obstructive pulmonary disorder, investigations of pulmonary pharmacology, or more general investigations of the genetic determinants of lung function. experimental animal models; pulmonary function test; allergy; bronchial asthma THE ABILITY TO MEASURE PULMONARY FUNCTION in individual mice on repeated occasions is of great interest because of the prominent role played by these animals in genetic and translational research into the causes and mechanisms of respiratory and allergic diseases such as asthma (5,14). It is becoming increasingly evident that the real potential of gene-or cellbased applications in experimental asthma models is tightly linked to a solid understanding of the underlying respiratory pathophysiology (20). This growing interest in functional studies with mice that correlate in vitro findings with in vivo determination of respiratory physiology has prompted the search for measurements of pulmonary function that are valid and easy to implement.Existing methods for measuring respiratory function in mice in vivo include noninvasive and invasive technologies (2, 5). Noninvasive determination of airway responsiveness (AR) in intact conscious mice has gained much interdisciplinary interest as a convenient and effective method for screening respiratory function over extended periods of time (11).Concerns with existing noninvasive methods in spontaneously ...

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Section: Discussionsupporting

confidence: 88%

Repetitive measurements of pulmonary mechanics to inhaled cholinergic challenge in spontaneously breathing mice

Glaab

Mitzner

Braun

et al. 2004

Journal of Applied Physiology

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“…Animal data on ageing and airway responsiveness are confusing. Both increases and decreases have been reported [20,21], some of which are certainly due to methodological discrepancies [22],…”

Section: Normal Infantsmentioning

confidence: 95%

Airway responsiveness in infancy

Silverman

1989

Clin Experimental Allergy

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“…Edema in peribronchovascular areas also increases viscoelastic properties. Airway hyperresponsiveness can be detected with a dose-response curve to methacholine or the antigen itself ( Wang et al, 1986 ). Both constant phase model and equation of motion can be used to describe respiratory function in these animals ( Bates, 2009 ) (see Figure 1 and section “Respiratory mechanics in animal models” above).…”

Section: Respiratory Mechanics In Animal Modelsmentioning

confidence: 99%

Lung Mechanics Over the Century: From Bench to Bedside and Back to Bench

et al. 2022

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Lung physiology research advanced significantly over the last 100 years. Respiratory mechanics applied to animal models of lung disease extended the knowledge of the workings of respiratory system. In human research, a better understanding of respiratory mechanics has contributed to development of mechanical ventilators. In this review, we explore the use of respiratory mechanics in basic science to investigate asthma and chronic obstructive pulmonary disease (COPD). We also discuss the use of lung mechanics in clinical care and its role on the development of modern mechanical ventilators. Additionally, we analyse some bench-developed technologies that are not in widespread use in the present but can become part of the clinical arsenal in the future. Finally, we explore some of the difficult questions that intensive care doctors still face when managing respiratory failure. Bringing back these questions to bench can help to solve them. Interaction between basic and translational science and human subject investigation can be very rewarding, as in the conceptualization of “Lung Protective Ventilation” principles. We expect this interaction to expand further generating new treatments and managing strategies for patients with respiratory disease.

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Methacholine-induced airway reactivity of inbred rats

Cited by 9 publications

References 0 publications

Repetitive measurements of pulmonary mechanics to inhaled cholinergic challenge in spontaneously breathing mice

Repetitive measurements of pulmonary mechanics to inhaled cholinergic challenge in spontaneously breathing mice

Airway responsiveness in infancy

Lung Mechanics Over the Century: From Bench to Bedside and Back to Bench

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