Metatranscriptomic Sequencing Reveals Host Species as an Important Factor Shaping the Mosquito Virome

Li, Cixiu; Liu, Shuqi; Zhou, Hong; Zhu, Wei; Cui, Mingxue; Li, Juan; Wang, Jiao; Liu, Jiangyun; Zhu, Jin; Li, Weiping; Bi, Yang; Carr, Michael J.; Holmes, Edward C.; Shi, Weifeng

doi:10.1128/spectrum.04655-22

Cited by 8 publications

(10 citation statements)

References 45 publications

(55 reference statements)

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“…We observed that while certain taxa of viruses are shared between different mosquito genera, some viral groups are restricted to specific mosquito genera. These findings align with previous studies, indicating that the composition of viral communities present in mosquitoes varies depending on the mosquito genus [ 57 , 58 , 59 ]. This supports the hypothesis that viral composition can be host-specific, implying that different mosquito species or genera may have unique viral associations.…”

Section: Discussionsupporting

confidence: 92%

Longitudinal Study of Viral Diversity Associated with Mosquito Species Circulating in Cambodia

Mohamed Ali,

Rakotonirina,

Heng

et al. 2023

Viruses

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Arthropod-borne viruses (arboviruses) pose a significant global health threat and are primarily transmitted by mosquitoes. In Cambodia, there are currently 290 recorded mosquito species, with at least 17 of them considered potential vectors of arboviruses to humans. Effective surveillance of virome profiles in mosquitoes from Cambodia is vital, as it could help prevent and control arbovirus diseases in a country where epidemics occur frequently. The objective of this study was to identify and characterize the viral diversity in mosquitoes collected during a one-year longitudinal study conducted in various habitats across Cambodia. For this purpose, we used a metatranscriptomics approach and detected the presence of chikungunya virus in the collected mosquitoes. Additionally, we identified viruses categorized into 26 taxa, including those known to harbor arboviruses such as Flaviviridae and Orthomyxoviridae, along with a group of viruses not yet taxonomically identified and provisionally named “unclassified viruses”. Interestingly, the taxa detected varied in abundance and composition depending on the mosquito genus, with no significant influence of the collection season. Furthermore, most of the identified viruses were either closely related to viruses found exclusively in insects or represented new viruses belonging to the Rhabdoviridae and Birnaviridae families. The transmission capabilities of these novel viruses to vertebrates remain unknown.

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Section: Discussionsupporting

confidence: 92%

Longitudinal Study of Viral Diversity Associated with Mosquito Species Circulating in Cambodia

Mohamed Ali,

Rakotonirina,

Heng

et al. 2023

Viruses

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“…To further investigate the concept of the "core virome" in mosquitoes [11,56,57], BMSV, BMRV, BMOV1, BMOV2, BMBV1 BMBV2 and Guangzhou sobemo-like virus were quantified in 182 individual mosquitoes as they were abundantly present in our pools. The closest relatives of these seven (novel) viruses all been previously detected in mosquitoes [11,52,54,55,57,58].…”

Section: Discussionmentioning

confidence: 94%

“…albopictus is dominated by more than one virus, in this study Ae. albopictus was dominated by one virus, Guangzhou sobemo-like virus [52][53][54][55]. The reason for this could be either methodological (differences in wetlab procedures or bio-informatics methods and used thresholds), or biological (difference in ISV carriage).…”

Section: Discussionmentioning

confidence: 99%

Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species

Mbigha Donfack,

De Coninck,

Ghogomu

et al. 2024

Preprint

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In Cameroon, Aedes mosquitoes transmit various arboviruses, posing significant health risks. We aimed to characterize the Aedes virome in southwestern Cameroon and identify potential core viruses which might be associated with vector competence. A total of 398 Aedes mosquitoes were collected from four locations (Bafoussam, Buea, Edea and Yaounde). Aedes albopictus dominated all sites except for Bafoussam where Aedes africanus prevailed. Metagenomic analyses of mosquitoes grouped per species into 54 pools revealed notable differences in eukaryotic viromes between Ae. africanus and Ae. albopictus, with the former exhibiting greater richness and diversity. Thirty-seven eukaryotic virus species from 16 families were identified, including six novel viruses with near complete genome sequences. Seven viruses were further quantified in individual mosquitoes via qRT-PCR. Although none of them could be identified as core viruses, Guangzhou sobemo-like virus and Bafoussam mosquito solemovirus virus were highly prevalent regionally in Ae. albopictus and Ae. africanus, respectively. This study highlights the diverse eukaryotic virome of Aedes species in southwestern Cameroon. Despite their shared Genus, Aedes species exhibit limited viral sharing, with varying viral abundance and prevalence across locations. Ae. africanus, an understudied vector, harbors a rich and diverse virome, suggesting potential implications for arbovirus vector competence.

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“…More than 35,000 individuals were collected in southern Manitoba over a three-year period (2019 to 2021) and subjected to metatranscriptomic analysis. This approach has been harnessed to catalogue the microorganisms harboured by mosquitoes from other geographical regions (Shi et al 2017;Sadeghi et al 2018;Batson et al 2021;Ortiz-Baez et al 2022;Li et al 2023); however, to our knowledge this is the first study done in Canada. A distinct advantage of RNA sequencing over 16S rRNA or shotgun metagenomics is the capability to detect RNA viruses (Wensel et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomic analysis of common mosquito vector species in the Canadian Prairies

Baril,

Cassone

2023

Preprint

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The microbiome plays vital roles in the life history of mosquitoes, including their development, immunity, longevity, and vector competence. Recent advances in sequencing technologies have allowed for detailed exploration into the diverse microorganisms harboured by these medically important insects. Although these meta-studies have catalogued the microbiomes of mosquitoes on several continents, much of the information currently available for North America is limited to the state of California. In this study, we collected >35,000 mosquitoes throughout Manitoba, Canada over a two-year period, and then harnessed RNA sequencing and targeted RT-PCR to characterize the microbiomes of the eight most pervasive and important vector and pest species. The consensus microbiome of each species was overwhelmingly composed of viruses, but also included fungi, bacteria, protozoa, and parasitic invertebrates. The microbial assemblages were heterogeneous between species, even within the same genus. We detected notable pathogens, including the causal agents of Cache Valley Fever, avian malaria, and canine heartworm. The remaining microbiome consisted largely of putatively insect-specific viruses that are not well characterized, including 17 newly discovered viruses from 10 different families. Future research should focus on evaluating the potential application of these viruses in biocontrol, as biomarkers, and/or in disrupting mosquito vectorial capacity. Interestingly, we also detected viruses that naturally infect honeybees and thrips, which were presumably acquired indirectly through nectar foraging behaviours. Overall, we provide the first comprehensive catalogue of the microorganisms harboured by the most common and important mosquito vectors and pests in the Canadian Prairies.

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Metatranscriptomic Sequencing Reveals Host Species as an Important Factor Shaping the Mosquito Virome

Cited by 8 publications

References 45 publications

Longitudinal Study of Viral Diversity Associated with Mosquito Species Circulating in Cambodia

Longitudinal Study of Viral Diversity Associated with Mosquito Species Circulating in Cambodia

Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species

Metatranscriptomic analysis of common mosquito vector species in the Canadian Prairies

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