Metatranscriptome profiling reveals versatile N-nutrient utilization, CO2 limitation, oxidative stress, and active toxin production in an Alexandrium fundyense bloom

Zhuang, Yunyun; Zhang, Huan; Hannick, Linda; Lin, Senjie

doi:10.1016/j.hal.2014.12.006

Cited by 61 publications

(49 citation statements)

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“…Further analysis of this pathway revealed that most dinoflagellates possess genes that permit the metabolic capability to utilize substrates such as acetate for carbohydrate biosynthesis and nitrogen via the ornithine-urea cycle and production of nitric oxide synthase [19]. Furthermore, EST analysis of Alexandrium fundyense strain CCMP 1719, originally isolated from the Gulf of Maine, revealed libraries encoding cyanate lyase also known as cyanase (cynS), which functions in the degradation of cyanate, a toxic nitrogen-containing compound, to produce carbon dioxide and ammonium [20]. The presence of an almost complete coding region of dinoflagellate-specific trans-spliced leader (DinoSL) in the cynS gene suggests that this gene was dinoflagellate originated and was likely functional [20][21][22][23].…”

Section: Dinoflagellates' Growth and Gene Regulationmentioning

confidence: 99%

“…Furthermore, EST analysis of Alexandrium fundyense strain CCMP 1719, originally isolated from the Gulf of Maine, revealed libraries encoding cyanate lyase also known as cyanase (cynS), which functions in the degradation of cyanate, a toxic nitrogen-containing compound, to produce carbon dioxide and ammonium [20]. The presence of an almost complete coding region of dinoflagellate-specific trans-spliced leader (DinoSL) in the cynS gene suggests that this gene was dinoflagellate originated and was likely functional [20][21][22][23]. However, it is still unclear whether A. fundyense takes up cyanate from the environment or uses cyanate intracellularly from spontaneous degradation of urea, as there is no cyanate transporter gene presence in the transcriptome data [20].…”

Section: Dinoflagellates' Growth and Gene Regulationmentioning

confidence: 99%

“…The presence of an almost complete coding region of dinoflagellate-specific trans-spliced leader (DinoSL) in the cynS gene suggests that this gene was dinoflagellate originated and was likely functional [20][21][22][23]. However, it is still unclear whether A. fundyense takes up cyanate from the environment or uses cyanate intracellularly from spontaneous degradation of urea, as there is no cyanate transporter gene presence in the transcriptome data [20]. Previously, Prorocentrum donghaiense demonstrated the ability to utilize cyanate as its sole nitrogen source [24].…”

Section: Dinoflagellates' Growth and Gene Regulationmentioning

confidence: 99%

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Current Knowledge and Recent Advances in Marine Dinoflagellate Transcriptomic Research

Akbar

Ali

Usup

et al. 2018

JMSE

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Abstract:Dinoflagellates are essential components in marine ecosystems, and they possess two dissimilar flagella to facilitate movement. Dinoflagellates are major components of marine food webs and of extreme importance in balancing the ecosystem energy flux in oceans. They have been reported to be the primary cause of harmful algae bloom (HABs) events around the world, causing seafood poisoning and therefore having a direct impact on human health. Interestingly, dinoflagellates in the genus Symbiodinium are major components of coral reef foundations. Knowledge regarding their genes and genome organization is currently limited due to their large genome size and other genetic and cytological characteristics that hinder whole genome sequencing of dinoflagellates. Transcriptomic approaches and genetic analyses have been employed to unravel the physiological and metabolic characteristics of dinoflagellates and their complexity. In this review, we summarize the current knowledge and findings from transcriptomic studies to understand the cell growth, effects on environmental stress, toxin biosynthesis, dynamic of HABs, phylogeny and endosymbiosis of dinoflagellates. With the advancement of high throughput sequencing technologies and lower cost of sequencing, transcriptomic approaches will likely deepen our understanding in other aspects of dinoflagellates' molecular biology such as gene functional analysis, systems biology and development of model organisms.

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Section: Dinoflagellates' Growth and Gene Regulationmentioning

confidence: 99%

Section: Dinoflagellates' Growth and Gene Regulationmentioning

confidence: 99%

Section: Dinoflagellates' Growth and Gene Regulationmentioning

confidence: 99%

See 1 more Smart Citation

Current Knowledge and Recent Advances in Marine Dinoflagellate Transcriptomic Research

Akbar

Ali

Usup

et al. 2018

JMSE

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“…Dinoflagellate-associated genes involved in N metabolism, including nitrate/ammonium transporters, nitrate/nitrite reductases, urease and glutamine and glutamate synthases were expressed in all sequence libraries, indicating that dinoflagellates are likely using multiple N sources (Zhuang et al, 2015; Figure 4). However, expression patterns revealed discrepancies in N metabolism between the bloom and the nonbloom samples.…”

Section: Molecular Insights Into a Dinoflagellate Bloommentioning

confidence: 99%

“…Also, recent realization of there being a high degree of inter-and intraspecific species interactions during rapidly changing bloom microenvironments contributes to the difficulty in identifying the physiological processes involved in bloom formation and longevity (Cole, 1982;Amin et al, 2015;Lima-mendez et al, 2015;Zhuang et al, 2015). Primary methods for examining phytoplankton dynamics within water quality monitoring programs typically have used bulk plankton community analyses (for example, chlorophyll a, primary productivity, nutrient uptake rates, etc.).…”

Section: Introductionmentioning

confidence: 99%

Molecular insights into a dinoflagellate bloom

et al. 2016

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In coastal waters worldwide, an increase in frequency and intensity of algal blooms has been attributed to eutrophication, with further increases predicted because of climate change. Yet, the cellular-level changes that occur in blooming algae remain largely unknown. Comparative metatranscriptomics was used to investigate the underlying molecular mechanisms associated with a dinoflagellate bloom in a eutrophied estuary. Here we show that under bloom conditions, there is increased expression of metabolic pathways indicative of rapidly growing cells, including energy production, carbon metabolism, transporters and synthesis of cellular membrane components. In addition, there is a prominence of highly expressed genes involved in the synthesis of membraneassociated molecules, including those for the production of glycosaminoglycans (GAGs), which may serve roles in nutrient acquisition and/or cell surface adhesion. Biotin and thiamine synthesis genes also increased expression along with several cobalamin biosynthesis-associated genes, suggesting processing of B 12 intermediates by dinoflagellates. The patterns in gene expression observed are consistent with bloom-forming dinoflagellates eliciting a cellular response to elevated nutrient demands and to promote interactions with their surrounding bacterial consortia, possibly in an effort to cultivate for enhancement of vitamin and nutrient exchanges and/or direct consumption. Our findings provide potential molecular targets for bloom characterization and management efforts.

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Cyanate distribution and uptake in North Atlantic coastal waters

Widner

Mulholland

2017

Limnology & Oceanography

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Cyanate (OCN−) is a reduced nitrogen compound that may be a source of nitrogen and carbon for marine phytoplankton. Cyanate is produced photochemically and through organic matter degradation but its distribution in marine systems is poorly defined because, until recently, there was no method to measure its concentration in marine environments. Here, we report results from the first regional oceanographic survey of cyanate distributions and uptake. Cyanate concentrations ranged from below detection (0.4 nM) to 11 nM in the coastal North Atlantic Ocean along the North American continental shelf and were slightly higher in nearshore surface waters than offshore surface waters. Subsurface cyanate maxima were observed at many stations suggesting a nonconservative distribution. Subsurface cyanate peak concentrations were tightly correlated with chlorophyll a concentrations integrated throughout the water column (R2 = 0.79). Cyanate N and C uptake were measurable in all regions and seasons sampled. Cyanate N uptake was significantly higher than cyanate C uptake for all cruises (1.3 ± 1.9 nmol L−1 h−1 and 0.4 ± 0.6 nmol L−1 h−1 for N and C, respectively). However, cyanate N uptake was significantly lower in November than in June and August, and cyanate C uptake was significantly higher in November than during June and August. Spatial trends in cyanate distribution and uptake, along with the correlation between depth‐integrated Chl a concentrations and cyanate maxima, suggest that cyanate is taken up in the euphotic zone and is likely produced by degradation of phytoplankton‐derived organic matter in subsurface waters.

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Metatranscriptome profiling reveals versatile N-nutrient utilization, CO2 limitation, oxidative stress, and active toxin production in an Alexandrium fundyense bloom

Cited by 61 publications

References 68 publications

Current Knowledge and Recent Advances in Marine Dinoflagellate Transcriptomic Research

Current Knowledge and Recent Advances in Marine Dinoflagellate Transcriptomic Research

Molecular insights into a dinoflagellate bloom

Cyanate distribution and uptake in North Atlantic coastal waters

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