Metatranscriptome analysis of the intestinal microorganisms in Pardosa pseudoannulata in response to cadmium stress

Yang, Huilin; Wang, Juan; Lv, Ziquan; Tian, Jianxiang; Peng, Yu; Peng, Xian‐Jin; Xu, Xiang; Song, Qisheng; Lv, Bo; Chen, Zhaoyang; Sun, Zheng; Wang, Zhi

doi:10.1016/j.ecoenv.2018.04.053

Cited by 30 publications

(15 citation statements)

References 62 publications

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“…Previous studies have examined the effects of cadmium on the microbes associated with mice (76) and spiders (44) and the effects of selenate on bumble bee microbes (35). Host-associated microbes can detoxify or sequester toxic metals, including arsenic (47), selenate (35), lead and chromium (48), copper (49), and cadmium (50)(51)(52).…”

Section: Discussionmentioning

confidence: 99%

“…There has been a fair amount of investigation into the interactions between cadmium and animal microbiomes (reviewed in reference 38). Previous work has shown that cadmium exposure significantly alters the microbiome of rats (39), mice (40)(41)(42), earthworms (43), and spiders (44). To date, no research has investigated these interactions in any insect species.…”

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confidence: 99%

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Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Rothman

Leger

Kirkwood

et al. 2019

Appl Environ Microbiol

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Honey bees are important insect pollinators used heavily in agriculture and can be found in diverse environments. Bees may encounter toxicants such as cadmium and selenate by foraging on plants growing in contaminated areas, which can result in negative health effects. Honey bees are known to have a simple and consistent microbiome that conveys many benefits to the host, and toxicant exposure may impact this symbiotic microbial community. We used 16S rRNA gene sequencing to assay the effects that sublethal cadmium and selenate treatments had over 7 days and found that both treatments significantly but subtly altered the composition of the bee microbiome. Next, we exposed bees to cadmium and selenate and then used untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics to show that chemical exposure changed the bees' metabolite profiles and that compounds which may be involved in detoxification, proteolysis, and lipolysis were more abundant in treatments. Finally, we exposed several strains of bee-associated bacteria in liquid culture and found that each strain removed cadmium from its medium but that only Lactobacillus Firm-5 microbes assimilated selenate, indicating the possibility that these microbes may reduce the metal and metalloid burden on their host. Overall, our report shows that metal and metalloid exposure can affect the honey bee microbiome and metabolome and that strains of bee-associated bacteria can bioaccumulate these toxicants. IMPORTANCE Bees are important insect pollinators that may encounter environmental pollution when foraging upon plants grown in contaminated areas. Despite the pervasiveness of pollution, little is known about the effects of these toxicants on honey bee metabolism and their symbiotic microbiomes. Here, we investigated the impact of selenate and cadmium exposure on the gut microbiome and metabolome of honey bees. We found that exposure to these chemicals subtly altered the overall composition of the bees' microbiome and metabolome and that exposure to toxicants may negatively impact both host and microbe. As the microbiome of animals can reduce mortality upon metal or metalloid challenge, we grew bee-associated bacteria in media spiked with selenate or cadmium. We show that some bacteria can remove these toxicants from their media in vitro and suggest that bacteria may reduce metal burden in their hosts.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Rothman

Leger

Kirkwood

et al. 2019

Appl Environ Microbiol

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“…P. fluorescens strain accumulates copper ions in quantities up to 500 mg/g of dry biomass [9], and the strain of P. mendocina sorbs copper in quantities up to 558 mg/g of dry biomass and cadmium to 0.75 mg/g of dry biomass [10]. P. pseudoalcaligenes reasonably well accumulates lead and copper ions, their number reach 271.7 mg/g and 46.8 mg/g of dry biomass, respectively [11,12].…”

Section: Resultsmentioning

confidence: 99%

A study on heavy metal sorption properties of intestinal microbiota in vitro

Sizentsov

Кван

et al. 2019

E3S Web Conf.

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Water and soil pollution by heavy metals has led to their accumulation in the ground and waters, to a sharp decline of ecosystem biopotential, and to foodstuff contamination, resulting in internal pollutions in bodies of humans and animals with food, air, water. In this regard, the purpose of our study was to examine the impact of heavy metals on the growth of representatives of rats’ intestinal microbiota and to estimate sorption characteristics of these microorganisms. The research was conducted in three stages: i) a study of essential and xenobiotic elements’ biotoxicity; ii) their impact on the growth of the microbiota in periodic cultivation; and iii) studying bioaccumulative characteristics. In the research, we discovered that all investigated organisms had pronounced resistance to lead and iron, which was confirmed by the results of the evaluating the impact on the growth and the accumulating characteristics of the studied microorganisms (more than 50%). The strain of E. faecium has the most pronounced sorption characteristics – its level of accumulation amounted for iron –63.3%, lead – 61%, zinc – 38.4%, copper – 11.4%, and cadmium – 33.2%, minimum values were obtained in samples of L. acidophilus, which sorbed these elements in concentrations of 46.2%, 42.3%, 10.3%, 3.8%, and 5.3%, respectively.

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“…While each factor has been examined separately in other species, the ecological consequences of their interactions are not yet known (Murray et al ., ). Furthermore, environmental stress can also affect the abundance of Wolbachia ; for example, Wolbachia has been shown to decrease in Pardosa pseudoannulata (Araneae: Lycosidae) under cadmium stress (Yang et al ., ). Consequently, the molecular mechanism of the combined effects of elevated CO 2 and Wolbachia infection remain unclear.…”

Section: Introductionmentioning

confidence: 97%

Transcriptome responses to elevated CO₂ level and Wolbachia‐infection stress in Hylyphantes graminicola (Araneae: Linyphiidae)

Wang

Deng

et al. 2019

Insect Science

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Hylyphantes graminicola is a resident spider species found in maize and cotton fields and is an important biological control agent of various pests. Previous studies have demonstrated that stress from elevated CO2 and Wolbachia infection can strongly affect spider species. Thus, based on CO2 levels (400 ppm, current atmospheric CO2 concentration and 800 ppm, high CO2 concentration) and Wolbachia status (Wolbachia‐infected, W+ and Wolbachia‐uninfected, W−), we divided H. graminicola individuals into four treatment groups: W− 400 ppm, W− 800 ppm, W+ 400 ppm, and W+ 800 ppm. To investigate the effects of elevated CO2 levels (W− 400 vs W− 800), Wolbachia infection (W− 400 vs W+ 400), and the interactions between these two factors (W− 400 vs W+ 800), high‐throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and identify stress‐related differentially expressed genes (DEGs). De novo assembly of complementary DNA sequences generated 86 688 unigenes, 23 938 of which were annotated in public databases. A total of 84, 21, and 157 DEGs were found among W− 400 vs W− 800, W− 400 vs W+ 400, and W− 400 vs W+ 800, respectively. Functional enrichment analysis revealed that metabolic processes, signaling, and catalytic activity were significantly affected by elevated CO2 levels and Wolbachia infection. Our findings suggest that the impact of elevated CO2 levels and Wolbachia infection on the H. graminicola transcriptome was, to a large extent, on genes involved in metabolic processes. This study is the first description of transcriptome changes in response to elevated CO2 levels and Wolbachia infection in spiders.

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Metatranscriptome analysis of the intestinal microorganisms in Pardosa pseudoannulata in response to cadmium stress

Cited by 30 publications

References 62 publications

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

A study on heavy metal sorption properties of intestinal microbiota in vitro

Transcriptome responses to elevated CO₂ level and Wolbachia‐infection stress in Hylyphantes graminicola (Araneae: Linyphiidae)

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Metatranscriptome analysis of the intestinal microorganisms in Pardosa pseudoannulata in response to cadmium stress

Cited by 30 publications

References 62 publications

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

A study on heavy metal sorption properties of intestinal microbiota in vitro

Transcriptome responses to elevated CO2 level and Wolbachia‐infection stress in Hylyphantes graminicola (Araneae: Linyphiidae)

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Transcriptome responses to elevated CO₂ level and Wolbachia‐infection stress in Hylyphantes graminicola (Araneae: Linyphiidae)