Metataxonomics, metagenomics and metabolomics analysis of the influence of temperature modification in full-scale anaerobic digesters

Puig‐Castellví, Francesc; Midoux, Cédric; Guenne, Angéline; Conteau, Delphine; Franchi, Óscar; Bureau, Chrystelle; Madigou, Céline; Bouveresse, Delphine Jouan-Rimbaud; Kroff, Pablo; Mazéas, Laurent; Rutledge, Douglas; Gaval, G.; Chapleur, Olivier

doi:10.1016/j.biortech.2021.126612

Cited by 20 publications

(4 citation statements)

References 48 publications

(61 reference statements)

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“…Microbial diversity increased after temperature downshifts to 48°C, as illustrated by Simpson's and Shannon-Wiener's indices; however, it decreased at 45°C incubation. Based on metataxonomics and metagenomics, Puig-Castellvi et al [46] suggested that a temperature decrease allows a significant increase in the sub-dominant bacterial population, thus destabilizing the microbial population and lowering biogas production.…”

Section: Dna Sequence and Microbial Diversity Analysismentioning

confidence: 99%

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

Sudiartha

Imai

2022

J. of Wat. & Envir. Tech.

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Operating temperature significantly affects biogas output, process stability, and microbial communities involved in anaerobic digestion. There are several unanswered questions regarding how microbial communities adapt in correlation with biogas production performance, especially when a digester fails to maintain thermophilic conditions. In this study, long-term lab-scale anaerobic digestion was carried out using two fed-batch reactors at 55°C, with subsequent decreases in temperature to 48°C and 45°C. Within the first month of incubation, methane (CH 4 ) production increased by approximately 11.18% following a reduction in temperature from 55°C to 48°C. However, the methane production decreased by 33% after the temperature was downshifted to 45°C. Despite the difference in methane production, the thermophilic methanogen population in both reactors declined significantly in the first month with a temperature decrease. After two months of incubation, these methanogenic communities recovered faster at 48°C than at 45°C, which was highlighted by the rapid colonization of Methanosaeta, Methanobacterium, and Methanothermobacter. Notably, Methanosaeta was the most abundant methanogen under all temperature conditions, indicating its thermotolerance.

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Section: Dna Sequence and Microbial Diversity Analysismentioning

confidence: 99%

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

Sudiartha

Imai

2022

J. of Wat. & Envir. Tech.

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“…The HPLC analysis employed an EC 100/2 NUCLEODUR HILIC analytical column (Macherey Nagel) with dimensions of 100 mm length, 2 mm inner diameter, and a particle size of 1.8 µm. Procedure is as described in [13] . Two mobile phases were utilized: solvent A, consisting of 50 mM ammonium acetate at pH 5.0, and solvent B, which was acetonitrile.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

A comprehensive dataset for assessing the impact of ammonium salts and zeolite on anaerobic digestion performance, microbial dynamics, and metabolomic profiles

Campuzano Zagal,

Wang,

Derlet

et al. 2024

Data in Brief

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“…During incubation, the following physicochemical parameters were monitored according to the protocols described in Puig-Castellví et al (2022): biogas production, biogas composition, pH, isotopic composition of the biogas, volatile fatty acids (VFAs), total inorganic carbon (TIC), total organic carbon (TOC) and chemical oxygen demand.…”

Section: Methodsmentioning

confidence: 99%

Diversity of novel archaeal viruses infecting methanogens discovered through coupling of stable isotope probing and metagenomics

Ngo

Enault

Midoux

et al. 2022

Environmental Microbiology

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Diversity of viruses infecting non‐extremophilic archaea has been grossly understudied. This is particularly the case for viruses infecting methanogenic archaea, key players in the global carbon biogeochemical cycle. Only a dozen of methanogenic archaeal viruses have been isolated so far. In the present study, we implemented an original coupling between stable isotope probing and complementary shotgun metagenomic analyses to identify viruses of methanogens involved in the bioconversion of formate, which was used as the sole carbon source in batch anaerobic digestion microcosms. Under our experimental conditions, the microcosms were dominated by methanogens belonging to the order Methanobacteriales (Methanobacterium and Methanobrevibacter genera). Metagenomic analyses yielded several previously uncharacterized viral genomes, including a complete genome of a head‐tailed virus (class Caudoviricetes, proposed family Speroviridae, Methanobacterium host) and several near‐complete genomes of spindle‐shaped viruses. The two groups of viruses are predicted to infect methanogens of the Methanobacterium and Methanosarcina genera and represent two new virus families. The metagenomics results are in good agreement with the electron microscopy observations, which revealed the dominance of head‐tailed virus‐like particles and the presence of spindle‐shaped particles. The present study significantly expands the knowledge on the viral diversity of viruses of methanogens.

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Metataxonomics, metagenomics and metabolomics analysis of the influence of temperature modification in full-scale anaerobic digesters

Cited by 20 publications

References 48 publications

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

A comprehensive dataset for assessing the impact of ammonium salts and zeolite on anaerobic digestion performance, microbial dynamics, and metabolomic profiles

Diversity of novel archaeal viruses infecting methanogens discovered through coupling of stable isotope probing and metagenomics

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