Metastatic malignant mesothelioma in a dog

Smith, D. A.; Hill, F. W.

doi:10.1016/0021-9975(89)90094-7

Cited by 20 publications

(28 citation statements)

References 5 publications

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“…Neoplastic cells present in serosal effusion can be seen as discrete cells or they form smaller or larger cellular aggregates, however as it has been found the pattern of cells aggregation does not correlate with tumor types (Bartazzolo et al 2012). According to the results of other studies, differentiation between mesotheliomas, epithelial malignant tumors, and some sarcomas can create diagnostic challenge (Smith and Hill 1989, Baker and Lumsden 2000, Brisson et al 2006, Ordonez 2006, Bertazollo et al 2012. Therefore, in most cases the final diagnosis of mesothelioma in animals is obtained by histology of tissue samples supported by additional immunohistochemical stains (Geninet et al 2003, Sato et al 2005, Brisson et al 2006, Bertazollo et al 2012.…”

Section: Discussionmentioning

confidence: 99%

Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs

Przeździecki¹,

Sapierzyński²

2014

Polish Journal of Veterinary Sciences

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The presence of tumor within the serosal cavities, often connected with accumulation of serosal effusion, is a quite common problem in the small animal veterinary medicine. The first step in diagnosis of such cases is cytopathological examination. The aim of the present study was to evaluate the usefulness of cytology and immunocytochemistry, using commercially available antibodies (anti-cytokeratin, anti-vimentin, and anti-desmin), in differential diagnosis of malignant tumors located within serosal cavities in dogs. The final cytological diagnosis of carcinoma/adenocarcinoma, sarcoma, and mesothelioma was obtained on the basis of routine cytopathology and immunocytochemistry, and then confirmed by histopathology and immunohistochemistry. Cytoplasmic immunoreactivitiy of normal mesothelid cells and cytoplasmic immunoreactivity of hyperplastic mesothelial cells revealed constant and strong expression of all examined intermediate filaments: cytokeratin, vimentin and desmin. Application of routine cytopathology and immunocytochemistry allowed 32 neoplastic tumors to be detected: 19 cases of carcinomas/adenocarcinomas, 6 cases of sarcomas, 7 cases of mesotheliomas. Immunostaining of cytopathological samples with chosen set of antibodies: anti-cytokeratin, anti-vimentin, anti-desmin is a useful, and low invasive test for differentiation between mesotheliomas and carcinomas/adenocarcinomas in dogs.

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Section: Discussionmentioning

confidence: 99%

Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs

Przeździecki¹,

Sapierzyński²

2014

Polish Journal of Veterinary Sciences

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“…Mesotheliomas are classified as benign or malignant. Reports of metastasis are rare, 8,11 but compression and constriction of organs surrounded by mesotheliomas and transplantation of neoplastic cells can result in death.…”

Section: Sclerosing Peritoneal Mesothelioma In a Dog Evaluated By Elementioning

confidence: 99%

Sclerosing Peritoneal Mesothelioma in a Dog Evaluated by Electron Microscopy and Immunoperoxidase Techniques

et al. 1992

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“…According to the results of numerous studies, differentiation between mesotheliomas, epithelial malignant tumours, and some sarcomas could be difficult or even impossible. It concerns not only cellular samples but also microscopic examination of tissue samples collected during more or less invasive medical procedures (Smith and Hill 1989, Baker and Lumsden 2000, Brisson et al 2006, Ordonez 2006, Gumber et al 2011, Bertazollo et al 2012, Przeździecki and Sapierzyński 2014. Due to moderate to severe pleomorphism of benign hyperplastic mesothelium this reactive process could be confused with some cancers.…”

Section: Discussionmentioning

confidence: 99%

Cytomorphometry of serosal effusion in dogs

Przeździecki¹,

Czopowicz²,

Sapierzyński³

2015

Polish Journal of Veterinary Sciences

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Cytomorphometry made on cytological slides is the quantitative method of precise analysis of cellular structures, including both cytoplasm and nucleus. The aim of this study was to describe cytomorphometric parameters of mesothelial cells in the course of benign reactive and malignant proliferation and to compare them to carcinomas and adenocarcinomas located within serosal cavities in dogs. The second aim was to evaluate applicability of cytomorphometry to diagnostics of diseases causing accumulation of effusion in serosal cavities. Cytological samples of normal and non-malignant mesothelium, mesothelioma and various carcinomas were collected from dogs. Cytomorphometry was made on the smears stained with Giemsa solution. Mean nuclear and cellular perimeter, mean nuclear and cellular area, mean nuclear and cellular diameter, and mean nuclear and cellular roundness were determined. Moreover, nuclear to cytoplasmic ratio (N/C) was calculated. The data revealed statistically significant differences for all parameters, excluding mean nuclear perimeter, between compared groups. Normal mesothelium cells and their nuclei were significantly smaller and more elongated than cells and nuclei of both benign reactive and malignant neoplastic mesothelium. Only a few differences were observed between benign reactive mesothelium cells and mesothelioma cells -mean nuclear area and mean nuclear diameter of benign reactive mesothelium cells were significantly larger and N/C ratio was higher in comparison to mesothelioma cells. Even though some significant differences were observed, considerable overlap of these cytomorphometric parameters in animals with different diseases limited practical role of these observations. Cytomorphometric analysis of cellular samples collected from dogs with proliferative processes affecting serosal cavities can be only an auxiliary method increasing accuracy of preoperative diagnosis.

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Metastatic malignant mesothelioma in a dog

Cited by 20 publications

References 5 publications

Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs

Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs

Sclerosing Peritoneal Mesothelioma in a Dog Evaluated by Electron Microscopy and Immunoperoxidase Techniques

Cytomorphometry of serosal effusion in dogs

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