Metastasis Suppressor Tetraspanin CD82/KAI1 Regulates Ubiquitylation of Epidermal Growth Factor Receptor

Odintsova, Elena; Niel, Guillaume van; Conjeaud, Hélène; Raposo, Graça; Iwamoto, Ryo; Mekada, Eisuke; Berditchevski, Fedor

doi:10.1074/jbc.m112.439380

Cited by 58 publications

(43 citation statements)

References 46 publications

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“…Depletion of both CD82 and TI-VAMP markedly increases the endocytosis of activated EGFR and decreases MAPK signaling, supporting the notion that CD82 facilitates EGFR coalescence with the microdomains controlling EGFR endocytosis and signaling [64]. CD82 reduces the EGFR ubiquitinylation following HB-EGF and amphiregulin stimulations and alters the trafficking kinetics of the ligand-stimulated EGFR to and from early endosomes in the manners dependent on C-Cbl, PKC, and CD82 C-terminal cytoplasmic domain [66].…”

Section: In Solid Tumor Cells Cd82 Regulates the Signaling Of Membrasupporting

confidence: 57%

Tetraspanin CD82: a suppressor of solid tumors and a modulator of membrane heterogeneity

Feng

Huang

Wren

et al. 2015

Cancer Metastasis Rev

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Tetraspanin CD82 suppresses the progression and metastasis of a wide range of solid malignant tumors. However, its roles in tumorigenesis and hematopoietic malignancy remain unclear. Ubiquitously expressed CD82 restrains cell migration and cell invasion by modulating both cell-matrix and cell-cell adhesiveness and confining outside-in pro-motility signaling. This restraint at least contributes to, if not determines, the metastasis-suppressive activity and, also likely, the physiological functions of CD82. As a modulator of cell membrane heterogeneity, CD82 alters microdomains, trafficking, and topography of the membrane by changing the membrane molecular landscape. The functional activities of membrane molecules and the cytoskeletal interaction of the cell membrane are subsequently altered, followed by changes in cellular functions. Given its pathological and physiological importance, CD82 is a promising candidate for clinically predicting and blocking tumor progression and metastasis and also an emerging model protein for mechanistically understanding cell membrane organization and heterogeneity.

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Section: In Solid Tumor Cells Cd82 Regulates the Signaling Of Membrasupporting

confidence: 57%

Tetraspanin CD82: a suppressor of solid tumors and a modulator of membrane heterogeneity

Feng

Huang

Wren

et al. 2015

Cancer Metastasis Rev

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“…The suspected reason for the susceptibility of MCF-7 to IMAB-AgNPs may depend on the size, charge, and shape of nanoparticles. Cytotoxicity effects of nanoparticles on target cells have been attributed to various mechanisms like particle-induced apoptosis (He et al 2004, Odintsova et al 2013, Sadat Shandiz et al 2015). …”

Section: Mtt Resultsmentioning

confidence: 99%

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

Shandiz

Ardestani

Shahbazzadeh

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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In the current study, in vitro biological feature of imatinib-loaded silver nanoparticles (IMAB-AgNPs) on human breast cancer cell line was investigated. The formation of synthesized silver nanoparticles (AgNPs) was characterized by UV-Visible spectroscopy, EDS, TEM imaging, SEM, FTIR, DLS and Zeta potentiometer. The developed IMAB-AgNPs with maximum percentage of loading efficiency was demonstrated in the average of 130 nm and mostly spherical. Additionally, in vitro drug release study showed a slow and continuous release of imatinib over a period of 80 h. We demonstrated that the synthesized IMAB-AgNPs exhibited a dose-dependent cytotoxicity against MCF-7 cell line. Then, real-time PCR method was also applied for the investigation of Bax and Bcl-2 gene expression in the cells. Comparing IMAB-AgNPs to AgNPs and Imatinib revealed the ability of IMAB-AgNPs to up-regulating Bax/Bcl-2 ratio. An induction of apoptosis was evidenced by Annexin-V/PI detection assay. Based on the current obtained data, the IMAB-AgNPs can exhibit inhibitory effect on viability through up regulation of apoptosis in MCF-7 cancer cells, which provides influencing evidence for the green synthesized AgNPs as a promising sustained drug delivery system.

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“…As a matter of fact, tetraspanins, including KAI1, are well known to be endowed with trafficking functions, possibly achieved via an endosomal-lysosomal sorting motif [6]. Moreover, others showed that KAI1 is capable of negatively acting on EGF-R ubiquitylation following cell stimulation by EGF or amphiregulin thereby affecting EGF-R degradation [47,81]. When we studied EGF-R expression as a function of KAI1-SP overexpression in the present study, we found enhanced EGF-R expression, also on the surface of human ovarian cancer cells.…”

Section: Effect Of Kai1-wt and Kai1-sp On Cell Migrationmentioning

confidence: 99%

Alternative splicing of KAI1 abrogates its tumor-suppressive effects on integrin αvβ3-mediated ovarian cancer biology

Upheber

Karle

Miller

et al. 2015

Cellular Signalling

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Metastasis Suppressor Tetraspanin CD82/KAI1 Regulates Ubiquitylation of Epidermal Growth Factor Receptor

Cited by 58 publications

References 46 publications

Tetraspanin CD82: a suppressor of solid tumors and a modulator of membrane heterogeneity

Tetraspanin CD82: a suppressor of solid tumors and a modulator of membrane heterogeneity

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

Alternative splicing of KAI1 abrogates its tumor-suppressive effects on integrin αvβ3-mediated ovarian cancer biology

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