Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10

Bao, Lili; You, Bo; Shi, Si; Shan, Ying; Zhang, Qicheng; Yue, Huijun; Zhang, Jie; Zhang, Wei; Shi, Yunwei; Liu, Yifei; Wang, Xin; Liu, Dong; You, Yiwen

doi:10.1038/s41388-018-0183-6

Cited by 172 publications

(163 citation statements)

References 50 publications

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“…The main therapeutic strategies for NPC patients are chemoradiotherapy and radiotherapy . Even though great progress has been made in diagnosis and prognosis of NPC, the morbidity and mortality are high owing to local recurrence and systemic metastasis . Therefore, a better understanding of the molecular mechanisms of NPC may improve the diagnosis and prognosis of NPC patients.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐103 promotes nasopharyngeal carcinoma through targeting TIMP‐3 and the Wnt/β‐catenin pathway

Zhao

Wang

2019

The Laryngoscope

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Objectives/Hypothesis To verify how microRNA‐103 (miR‐103) is involved in nasopharyngeal carcinoma (NPC) development. Study Design Research on the relationship between miR‐103 and NPC. Methods Tissue inhibitor of metalloproteinases‐3 (TIMP‐3) was identified as the theoretical target gene of miR‐103, and its regulatory mechanism in NPC was explored by quantitative reverse transcription polymerase chain reaction, Western blot, MTT, transwell, and luciferase reporter assays. Results MiR‐103 was upregulated whereas TIMP‐3 was markedly decreased in NPC tissue samples. Ectopic expression of miR‐103 promoted NPC cell viability, migration, and invasion. In vitro assay showed that TIMP‐3 recovered miR‐103–mediated promotion of NPC cell viability, migration, and invasion. The expression of Wnt/β‐catenin pathway markers (β‐catenin and cyclin D1) were enhanced after miR‐103 overexpression. Conclusions MiR‐103 might play a key role in NPC carcinogenesis by targeting TIMP‐3 and affecting the Wnt/β‐catenin pathway. Level of Evidence NA Laryngoscope, 130:E75–E82, 2020

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Section: Introductionmentioning

confidence: 99%

MicroRNA‐103 promotes nasopharyngeal carcinoma through targeting TIMP‐3 and the Wnt/β‐catenin pathway

Zhao

Wang

2019

The Laryngoscope

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“…Studies have shown that miR-23a can be carried by tumor cell-secreted exosomes such as nasopharyngeal carcinoma or lung cancer cells and then exert their regulatory functions on angiogenesis (22)(23)(24), which suggested that miR-23a carried by GC cell-derived exosomes might be involved in angiogenesis. To validate this hypothesis, exosomes were extracted from GES-1, NCI-N87, HGC-27, AGS, and MKN45 using the ExoQuick method and then identified.…”

Section: Mir-23a Is Enriched In Gc Cells-derived Exosomesmentioning

confidence: 99%

Gastric Cancer Cell-Derived Exosomal microRNA-23a Promotes Angiogenesis by Targeting PTEN

Liu

et al. 2020

Front. Oncol.

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Hypoxia-exposed lung cancer-released exosomal microRNA-23a (miR-23a) has been shown to enhance angiogenesis as well as vascular permeability, contributing to the close correlation between exosomal miR-23a and tumorigenesis. The current study aimed to investigate whether gastric cancer (GC) cell-derived exosomal miR-23a could induce angiogenesis and to elucidate the potential mechanisms associated with the process. Differentially expressed miRNAs in GC were initially screened from the Gene Expression Omnibus database. Target genes were selected following miRNA-mRNA prediction and subsequently verified by dual luciferase reporter assay. RT-qPCR was conducted to detect miR-23a and PTEN expression in GC tissues, cells and exosomes. Human umbilical venous endothelial cells (HUVECs) were co-cultured with GC cell-derived exosomes to assess the angiogenesis mediated by exosomes in vitro. Additionally, PTEN was overexpressed in HUVECs to analyze the mechanism by which miR-23a regulates angiogenesis. miR-23a was highly expressed in GC tissues and cells and GC cell-derived exosomes. Angiogenesis was promoted by the co-culture of HUVECs and GC cells-derived exosomes, as evidenced by the increased expression of VEGF but decreased expression of TSP-1. PTEN was targeted by miR-23a and was lowly expressed in GC tissues. In a co-culture system, miR-23a carried by GC cells-derived exosomes promoted angiogenesis via the repression of PTEN. Collectively, GC cell-derived exosomal miR-23a could promote angiogenesis and provide blood supply for growth of GC cells. This study contributes to advancement of miRNA-targeted therapeutics.

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“…Angiogenesis is not a simple one‐to‐one correspondence regulated by exosomal miRNAs. Bao et al . detected and identified that highly enriched miR‐23a regulated angiogenesis by targeting gene TSGA10 in nasopharyngeal carcinoma (NPC; Table ).…”

Section: Exosome and Tumor Developmentmentioning

confidence: 99%

“…Angiogenesis is not a simple one-to-one correspondence regulated by exosomal miRNAs. Bao et al 49 detected and identified that highly enriched miR-23a regulated angiogenesis by targeting gene TSGA10 in nasopharyngeal carcinoma (NPC; Table 1). Under the hypoxic circumstance, Hsu et al 50 found that lung cancer-derived suppressing miR-23a in exosomes could upregulate prolyl hydroxylase 1 and 2 (PHD1 and 2), accumulated hypoxia-inducible factor-1 alpha (HIF-1 alpha) in endothelial cells so as to induce angiogenesis and promote vascular permeability ( Table 1).…”

Section: Cancer Metastasismentioning

confidence: 99%

The cancer exosomes: Clinical implications, applications and challenges

Tang

Hou

et al. 2019

Intl Journal of Cancer

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The exosome is a small functional vesicle enriched in selected proteins, lipids and nucleic acids, displaying distinct molecular heterogeneity. Exosomes released can transform the extracellular matrix microenvironments, transmit signals and molecules to recipient cells and trigger changes in their pathophysiological functions. Tumor‐derived exosomes mediate the interactions of tumor cells and microenvironment significantly, and they stimulate tumor growth and development through specific signaling pathways related to metastasis, therapeutic resistance and immunosuppression. Exosome biogenesis from tumors often represents abundant biological information, and novel and efficient isolation and detection methods of exosomes provide a promising approach for tumor diagnosis and prognosis estimation. Moreover, exosome can even be developed as therapeutic agents for multiple disease models based on effective material transport characteristics and biofilm specificity. This review reports the clinical implications and challenges of exosomes in cancer progression, therapy resistance, metastasis and immune escape, and underlying cancerogenic pathological phenotypes including fibrosis and viral infection.

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Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10

Cited by 172 publications

References 50 publications

MicroRNA‐103 promotes nasopharyngeal carcinoma through targeting TIMP‐3 and the Wnt/β‐catenin pathway

MicroRNA‐103 promotes nasopharyngeal carcinoma through targeting TIMP‐3 and the Wnt/β‐catenin pathway

Gastric Cancer Cell-Derived Exosomal microRNA-23a Promotes Angiogenesis by Targeting PTEN

The cancer exosomes: Clinical implications, applications and challenges

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