Metarhiziumanisopliae (Metchnikoff) Sorokin as an alternative to chemical pesticides against Odontotermesobesus (Rambur) in the era of organic agriculture

Abstract

Odontotermesobesus (Rambur) is one of the most destructive termite species to most of the cultivated crops. Against which a bioassay test with Metarhiziumanisopliae (Metchnikoff) Sorokin was conducted in the laboratory at nine different concentrations ranging from 1×10 3 to 1×10 11 conidia/ml. Three different medium that is termitorium soil, sterilised soil and Whatman No.1 filter paper based medium were used for the bioassay. Conidial suspension of M. anisopliae when treated to termite on termitorium soil based medium was more pathogenic and could cause highest mortality (83.88 %) of the termites with a week. Conidia of the fungus were found to burst out from the host body after 7th day of treatment. Infected termites showed progressive symptoms of sluggishness as compared with control. Mortality of termites showed presence of black melanisation spot on the cuticle.

Efficacy of Three Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii Isolates against Black Bean Aphid, Aphis fabae (Scop.) (Hemiptera: Aphididae) on Faba bean (Vicia faba L.)

“…Every plant infected with aphids was sprayed with an aqueous suspension containing 1  10 10 spores conidia ml -1 per EPF isolates by a hand atomize. Dead aphids were collected daily and transferred to a moist filter paper in a self-sealed Petri dish; once the dead aphid produced mycelial growth, they were considered for the mortality count (Pegu et al, 2017). The mortality data were then corrected using Abbott's formula (Abbott 1925).…”