Metarhizium humberi sp. nov. (Hypocreales: Clavicipitaceae), a new member of the PARB clade in the Metarhizium anisopliae complex from Latin America

Luz, Christian; Rocha, Luiz F.N.; Montalva, Cristian; Souza, Daniela Aguiar; Botelho, Ana Beatriz Riguetti Zanardo; Lopes, Rogério Biaggioni; Faria, Marcos; Delalibera, Ítalo

doi:10.1016/j.jip.2019.107216

Cited by 35 publications

(37 citation statements)

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“…The DR5 system has been widely used, as it provides a high level of accuracy concerning the sensitivity of auxin concentration and is a reliable evaluation for the presence of auxin (Chen et al, 2013). Although M. humberi is a sister lineage of the M. anisopliae s.str (Luz et al, 2019), the results obtained indicated that its performance is more similar to M. robertsii than to M. anisopliae in many aspects, including the production of key biomolecules that could be associated to endophytic colonization of plants and the antagonism of plant pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…One isolate of each of the endophytic entomopathogenic fungi M. robertsii, M. humberi, and M. anisopliae (Table 1) were used in this study. The isolates were deposited in "Prof. Sérgio Batista Alves" collection at ESALQ/USP and had been previously identified to species level by molecular techniques (Rezende et al, 2015;Botelho et al, 2019;Luz et al, 2019). M. robertsii and M. humberi, which are registered as biopesticides in Brazil, were selected for their effects on growth promotion of sugarcane plants (length of plants, increased roots, increase in chlorophyll content, and antagonism to sugarcane phytopathogens, and insect pests), which were demonstrated in our previous greenhouse and field studies (A.C.O.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…isolates, including the production of IAA, siderophores, chitinases, phytases, and phosphatases for phosphorus solubilization, alongside the effects on tomato miniature cultivar Micro-Tom (MT), inoculated with two root colonizers, M. robertsii and M. humberi sp. nov. Luz et al (2019), on growth promotion, fruit yield, and auxin accumulation in roots. The MT transgenic reporter tomato line is a useful phenotype to investigate IAA signaling through visualization of root parts based on the activity of β-glucuronidase (Blanco et al, 1982), confirming that both M. humberi and M. robertsii induced endogenous IAA production.…”

Section: Introductionmentioning

confidence: 99%

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Multi-Trait Biochemical Features of Metarhizium Species and Their Activities That Stimulate the Growth of Tomato Plants

Siqueira

Mascarin

Gonçalves

et al. 2020

Front. Sustain. Food Syst.

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The interaction between Metarhizium spp. and plant roots may induce host plant growth and help control below-and above-ground pests and diseases. We conducted in vivo and in vitro bioassays using native Brazilian isolates of Metarhizium robertsii (ESALQ 1635), M. humberi (ESALQ 1638), and M. anisopliae (ESALQ 1669) to better understand the possible mechanisms related to plant growth promotion traits and colonization of the root system in the model pot-grown tomato (Solanum lycopersicum L.) miniature cultivar "Micro-Tom." In vivo trials revealed that M. robertsii (ESALQ 1635) or M. humberi (ESALQ 1638) inoculated in tomato seedlings improved vegetative and reproductive traits. Inoculation with M. robertsii yielded taller plants, longer roots, and more shoot and root dry mass than M. humberi. The number of flowers and the fresh weight of fruits was significantly increased by M. robertsii and M. humberi inoculation, in relation to uninoculated control plants. Both fungal species endophytically colonized all tomato tissues within 30 days of inoculation. Plants inoculated with either M. robertsii or M. humberi increased auxin-induced GUS expression in the roots for up to 30 days after inoculation, confirming that Metarhizium induces auxin-regulated gene expression. We also explored the production of key compounds including enzymes, hormones, and metabolites involved in plant growth promotion. The three Metarhizium species grown with or without exogenous tryptophan were able to produce indole-3-acetic acid (IAA) at different titers. All Metarhizium isolates produced phosphatases, phytases, siderophores, and chitinases. Of particular importance, the M. robertsii and M. humberi isolates exhibited similar in vitro biochemical profiles, whereas M. anisopliae and Trichoderma harzianum isolates demonstrated distinct traits from the others. Taken together, we argue that the M. robertsii isolate is more efficient than the M. humberi isolate to endophytically colonize tomato plants resulting in improved growth. However, M. humberi (ESALQ 1638) yielded a slightly better production of some metabolites in vitro. Thus, Siqueira et al. Endophytic Multifunctional Metarhizium-Based Bioproduct we propose that the isolates of M. robertsii and M. humberi could be explored as complementary plant growth promoters.

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Section: Discussionmentioning

confidence: 99%

Section: Fungal Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multi-Trait Biochemical Features of Metarhizium Species and Their Activities That Stimulate the Growth of Tomato Plants

Siqueira

Mascarin

Gonçalves

et al. 2020

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

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“…Characterisation was based on sequencing of the ITS region and two genes, consisting of the internal transcribed spacer (ITS) (primers ITS1and ITS4), the partial tubulin (BtuB) (primers Bt2a and Bt2b) and the partial EF-1α (primers EF1F and EF2R) [12,13]. The PCR thermocycle conditions accorded with the technique used by [14].…”

Section: Molecular Identificationmentioning

confidence: 99%

“…Phylogenetic analyses were conducted using the dataset from Rehner and Kepler (2017) [13] and Luz et a. (2019) [12] , combining sequences of three loci (ITS, BT, tef1), so as to identify the species concerned. The alignments were done employing ClustalX, using the L-INS-I 6 option.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Characterisation ofMetarhizium majus(Hypocreales: Clavicipitaceae) isolated from the Western Cape province, South Africa

Mathulwe

Jacobs

Malan

et al. 2020

Preprint

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Entomopathogenic fungi (EPF) are important soil-dwelling entomopathogens, which can be used as biocontrol agents against pest insects. During a survey of the orchard soil at an organic farm, the EPF were identified to species level, using both morphological and molecular techniques. The EPF were trapped from soil samples, taken from an apricot orchard, which were baited in the laboratory, using susceptible host insects. The identification of Metarhizium majus from South African soil, using both morphological and molecular techniques, is verified. The occurrence of M. majus in the South African soil environment had not previously been reported.

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Optimization of granular formulations of Metarhizium humberi microsclerotia with humectants

et al. 2021

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Granular microsclerotial formulations of entomopathogenic fungi deserve attention because of their post-application, in situ production of new conidia that enhance and prolong mycoinsecticidal efficacy against a target pest insect. Because high ambient moisture is a crucial condition to induce fungal development and conidiogenesis on granules, we tested the impacts of the additions of three humectants -glycerin, propylene glycol, and polyethylene glycol 400-on water absorption by pellets incorporating microsclerotia of Metarhizium humberi IP 46 with microcrystalline cellulose or vermiculite carriers, and on the production of infective conidia of IP 46 microsclerotia in ambient humidities suboptimal for routine conidiogenesis. Glycerin facilitated greater and faster absorption of water than the other humectants. Microcrystalline cellulose absorbed low quantities of water without any added humectant whereas vermiculite did not. IP 46 did not grow or sporulate on pellets prepared with or without glycerin at 86% relative humidity (RH) or on control pellets without glycerin at 91% RH; conidial production on pellets prepared with vermiculite or microcrystalline cellulose and 10% glycerin reached 1.1 × 10 5 conidia/mg and 1 × 10 5 conidia/mg, respectively, after 20 days of exposure at 91% RH. Hence, these results strongly support glycerin as a suitable humectant for granular microsclerotial formulations of this fungus.

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Metarhizium humberi sp. nov. (Hypocreales: Clavicipitaceae), a new member of the PARB clade in the Metarhizium anisopliae complex from Latin America

Cited by 35 publications

References 50 publications

Multi-Trait Biochemical Features of Metarhizium Species and Their Activities That Stimulate the Growth of Tomato Plants

Multi-Trait Biochemical Features of Metarhizium Species and Their Activities That Stimulate the Growth of Tomato Plants

Characterisation ofMetarhizium majus(Hypocreales: Clavicipitaceae) isolated from the Western Cape province, South Africa

Optimization of granular formulations of Metarhizium humberi microsclerotia with humectants

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