Metaplasia of Chondrocytes into Osteoblasts

Włodarski, K; Włodarski, Paweł; Brodzikowska, Aniela

doi:10.3409/173491606778557491

Cited by 10 publications

(13 citation statements)

References 41 publications

(34 reference statements)

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“…Terminal differentiation of chondrocytes without cell enlargement has been suggested previously,15 though not in relation to bony spurs. Furthermore, even where chondrocytic hypertrophy does occur during ossification, cell enlargement is not necessarily followed by cell death 16.…”

Section: Discussionmentioning

confidence: 78%

Evidence for a distinctive pattern of bone formation in enthesophytes

Benjamin

Toumi

Suzuki

et al. 2008

Annals of the Rheumatic Diseases

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Section: Discussionmentioning

confidence: 78%

Evidence for a distinctive pattern of bone formation in enthesophytes

Benjamin

Toumi

Suzuki

et al. 2008

Annals of the Rheumatic Diseases

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“…In fact, earlier morphological, histological [147], and cell culture experiments [158, 160–162] all observed the presence of potential chondrocyte-derived osteoblasts and likely transdifferntiation events. It was suggested that hypertrophic chondrocytes to osteoblasts transdifferentiation might have occurred through either asymmetrical division or direct transdifferentiation [147] [162] [163] [164]. However, most of such evidence came from in vitro studies and was not fully conclusive.…”

Section: Chondrocyte Terminal Fates and Transdifferentiationmentioning

confidence: 99%

Signaling pathways regulating cartilage growth plate formation and activity

Samsa

Zhou

2017

Seminars in Cell & Developmental Biology

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The growth plate is a highly specialized and dynamic cartilage structure that serves many essential functions in skeleton patterning, growth and endochondral ossification in developing vertebrates. Major signaling pathways initiated by classical morphogens and by other systemic and tissuespecific factors are intimately involved in key aspects of growth plate development. As a corollary of these essential functions, disturbances in these pathways due to mutations or environmental factors lead to severe skeleton disorders. Here, we review these pathways and the most recent progress made in understanding their roles in chondrocyte differentiation in growth plate development and activity. Furthermore, we discuss newly uncovered pathways involved in growth plate formation, including mTOR, the circadian clock, and the COP9 signalosome.

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“…However, it is unclear whether transdifferentiation of chondrocytes into mature osteoblasts is fundamental to the process of fracture repair (Wlodarski et al . ).…”

Section: Introductionmentioning

confidence: 97%

Inducible cell labeling and lineage tracking during fracture repair

Seime

Kolind

Mikulec

et al. 2014

Develop. Growth Differ.

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Mouse models incorporating inducible Cre-ER T2 /LoxP recombination coupled with sensitive fluorescent reporter lines are being increasingly used to track cell lineages in vivo. In this study we use two inducible reporter strains, Ai9 iCol2a1 (Ai9 9 Col2a1-creER T2 ) to track contribution of chondrogenic progenitors during bone regeneration in a closed fracture model and Ai9 iUBC (Ai9 9 UBC-creER T2 ) to examine methods for inducing localized recombination. By comparing with Ai9 littermate controls as well as inducible reporter mice not dosed with tamoxifen, we revealed significant leakiness of the CreER T2 system, particularly in the bone marrow of both lines. These studies highlight the challenges associated with highly sensitive reporters that may be activated without induction in tissues where the CreER T2 fusion is expressed. Examination of the growth plate in the Ai9 iCol2a1 strain showed cells of the osteochondral lineage (cell co-staining with chondrocyte and osteoblast markers) labeled with the tdTom reporter. However, no such labeling was noted in healing fractures of Ai9 iCol2a1 mice. Attempts to label a single limb using intramuscular injection of 4-hydroxytamoxifen in the Ai9 iUBC strain resulted in complete labeling of the entire animal, comparable to intraperitoneal injection. While a challenge to interpret, these data are nonetheless informative regarding the limitations of these inducible reporter models, and justify caution and expansive controls in future studies using such models.

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Metaplasia of Chondrocytes into Osteoblasts

Cited by 10 publications

References 41 publications

Evidence for a distinctive pattern of bone formation in enthesophytes

Evidence for a distinctive pattern of bone formation in enthesophytes

Signaling pathways regulating cartilage growth plate formation and activity

Inducible cell labeling and lineage tracking during fracture repair

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