Metallotolerant Bacteria: Insights into Bacteria Thriving in Metal-Contaminated Areas

Barman, Dina; Jha, Dhruva Kumar; Bhattacharjee, Kaushik

doi:10.1007/978-981-15-3028-9_9

Cited by 9 publications

(5 citation statements)

References 108 publications

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“…Similarly, Actinobacteria have been reported in other ARD environments, including 90 microbial communities in a copper tailing impoundment in Anhui Province, China [ 50 , 52 , 53 ]. Both Proteobacteria and Actinobacteria thrive in diverse sediments and have evolved mechanisms to inhabit metal-rich environments [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of an acid rock drainage microbiome and transcriptome at the Ely Copper Mine Superfund site

et al. 2020

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The microbial oxidation of metal sulfides plays a major role in the formation of acid rock drainage (ARD). We aimed to broadly characterize the ARD at Ely Brook, which drains the Ely Copper Mine Superfund site in Vermont, USA, using metagenomics and metatranscriptomics to assess the metabolic potential and seasonal ecological roles of microorganisms in water and sediment. Using Centrifuge against the NCBI "nt" database,~25% of reads in sediment and water samples were classified as acid-tolerant Proteobacteria (61 ± 4%) belonging to the genera Pseudomonas (2.6-3.3%), Bradyrhizobium (1.7-4.1%), and Streptomyces (2.9-5.0%). Numerous genes (12%) were differentially expressed between seasons and played significant roles in iron, sulfur, carbon, and nitrogen cycling. The most abundant RNA transcript encoded the multidrug resistance protein Stp, and most expressed KEGG-annotated transcripts were involved in amino acid metabolism. Biosynthetic gene clusters involved in secondary metabolism (BGCs, 449) as well as metal-(133) and antibiotic-resistance (8501) genes were identified across the entire dataset. Several antibiotic and metal resistance genes were colocalized and coexpressed with putative BGCs, providing insight into the protective roles of the molecules BGCs produce. Our study shows that ecological stimuli, such as metal concentrations and seasonal variations, can drive ARD taxa to produce novel bioactive metabolites.

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Section: Resultsmentioning

confidence: 99%

Characterization of an acid rock drainage microbiome and transcriptome at the Ely Copper Mine Superfund site

et al. 2020

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“…Previous studies have reported that microorganisms from heavy-metal-contaminated areas contain metal-resistant genes, which express numerous proteins to hydrolyze and utilize heavy metal to survive (Barman et al, 2020). Moreover, studies have revealed that gram-negative and gram-positive bacteria exhibit distinct mechanisms to cope with high organic solvent contamination.…”

Section: Discussionmentioning

confidence: 99%

Plastic-degrading bacteria isolated from contaminated mangrove sediment in Wonorejo, Surabaya

Shovitri,

Hefdiyah,

Antika

et al. 2023

Appl Env Biotechnol

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Plastics have become inevitable needs in modern society due to their attractive properties, including thermostability, lightweight, flexibility, superior insulation, and low cost, which have led to massive production. Their persistence and challenges in disposal have detrimental effects on the environment leading to the development of a promising degradation process which is efficient, time-saving, and cost-effective. This study focused on discovering the potential plastic-degrading bacteria isolated from plastic-contaminated mangrove sediment in the Wonorejo area. We buried a commercially available plastic bag in the polluted mangrove sediment for 16 weeks. Our results showed that indigenous bacteria formed a biofilm on the plastic surface leading to a plastic dry weight loss of up to 12%. FTIR analysis revealed obvious transmittance attenuations in the buried plastic polymer, suggesting that their chemical properties may have been interrupted due to bacterial activity. Further, bacteria isolation and biochemical screening revealed that they were primarily dominated by Bacillus. According to 16S rRNA sequencing, they were identified as Brevibacilllus (BIO-B), Stenotrophomonas (BIO-G), and Lysinibacillus (SOI-C). The three genera mentioned earlier exhibited a detectable level of plastic-degrading activity and possessed lipolytic, ligninolytic, and alkane-degrading activities. Stenotrophomonas (BIO-G) showed a degradation activity on low-density polyethylene (LDPE) represented by a plastic dry weight loss of up to 8.9% within 4 weeks. As expected, plastic treated with BIO-G showed transmittance attenuation in FTIR analysis, albeit with a lower percentage than that treated with indigenous bacteria. Moreover, SEM analysis reveals changes in the morphological surface of plastic. Together, FTIR and SEM analysis indicated that bacteria disrupt both the chemical structure and morphological appearance of plastic polymer upon degradation process. These results denote that BIO-G indeed composes the aforementioned indigenous bacteria from polluted mangrove sediment. Thus, our study suggests the indigenous bacteria isolated from contaminated areas produced plastic-degrading enzymes and secreted to the environment to break down plastic compounds.

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“…Plasmids comprise up to 4.8% of LAB total gene content and are important for growth in the diverse, yet specific environments where these bacteria are found (Makarova et al 2006). Lactobacillus have been shown to be metallotolerant and have the ability to bind heavy metals and protect against metal-induced oxidative stress (Li et al 2017; Liu et al 2019; Barman et al 2020). Their abundance in serpentine soil treatments may reflect these phenotypic properties as serpentine soils have high concentrations of heavy metals.…”

Section: Discussionmentioning

confidence: 99%

Plant phenology influences rhizosphere microbial community and is accelerated by serpentine microorganisms inPlantago erecta

Igwe

Quasem

Liu

et al. 2021

Preprint

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Serpentine soils are drought-prone and rich in heavy metals, and plants growing on serpentine soils host distinct microbial communities that may affect plant survival and phenotype. However, whether the rhizosphere communities of plants from different soil chemistries are initially distinct or diverge over time may help us understand drivers of microbial community structure and function in stressful soils. Here, we test the hypothesis that rhizosphere microbial communities will converge over time (plant development), independent of soil chemistry and microbial source. We grew Plantago erecta in serpentine or nonserpentine soil, with serpentine or nonserpentine microbes and tracked plant growth and root phenotypes. We used 16S rRNA barcoding to compare bacterial species composition at seedling, vegetative, early-, and late-flowering phases. Plant phenotype and rhizosphere bacterial communities were mainly structured by soil type, with minor contributions by plant development, microbe source and their interactions. Serpentine microorganisms promoted early flowering in plants on non-serpentine soils. Despite strong effects of soil chemistry, the convergence in bacterial community composition across development demonstrates the importance of the plant-microbe interactions in shaping microbial assembly processes across soil types.

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Metallotolerant Bacteria: Insights into Bacteria Thriving in Metal-Contaminated Areas

Cited by 9 publications

References 108 publications

Characterization of an acid rock drainage microbiome and transcriptome at the Ely Copper Mine Superfund site

Characterization of an acid rock drainage microbiome and transcriptome at the Ely Copper Mine Superfund site

Plastic-degrading bacteria isolated from contaminated mangrove sediment in Wonorejo, Surabaya

Plant phenology influences rhizosphere microbial community and is accelerated by serpentine microorganisms inPlantago erecta

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