Metallothionein Genes and Expression for Heavy Metal Resistance

Nedkovska, M.; Atanassov, A.

doi:10.1080/13102818.1998.10818980

Cited by 12 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These inorganic, nonbiodegradable pollutants include lead, chromium, cadmium, mercury, copper, selenium, zinc, manganese, cobalt, nickel, silver, and so on [4]. Different organisms have various mechanisms for removing heavy metals and reducing their toxicity that one of them is MT protein [4,27]. MT is a widespread, small, and cysteine‐rich protein that binds to essential and nonessential heavy metals [28,29].…”

Section: Discussionmentioning

confidence: 99%

Expression regulation of chitin‐binding protein and metal‐binding peptide in new Bacillus velezensis: MALDI‐TOF MS/MS analysis

Etemadzadeh

Emtiazi

2021

J Basic Microbiol

View full text Add to dashboard Cite

Metallothionein and metal‐binding peptides are small cysteine‐rich proteins produced by different organisms in stress conditions. In this study, the metal‐binding peptide was detected in extracellular proteins of a new Bacillus velezensis strain, isolated from metal contaminated soil, and grown on the lead‐enriched medium, for the first time. The presence of sulfide peptide was assayed by two simple tests (lead sulfide and Ellman's reagent test) for preliminary, and subsequently confirmed using polyacrylamide gel electrophoresis at media with different lead concentrations that the low‐molecular‐weight protein fragments (≈10 kDa) were observed while none were detected in the medium containing sodium chloride or calcium salt. The amino acids of the observed fragments were analyzed by matrix‐assisted laser desorption ionization time‐of‐flight tandem mass spectrometry (MALDI‐TOF MS/MS). Also, the metal adsorption was confirmed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) by staining with chromium solution. The results showed that the putative sulfide peptide is metallothionein, which is induced in stress conditions. It was interesting that in all SDS profiles, one protein fragment (≈18 kDa) was inhibited in lead‐enriched media. The data from MALDI‐TOF MS/MS analysis showed that this fraction was a chitin‐binding protein whose production was regulated by metal contamination. It is anticipated that these two proteins regulate the toxicity of lead.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression regulation of chitin‐binding protein and metal‐binding peptide in new Bacillus velezensis: MALDI‐TOF MS/MS analysis

Etemadzadeh

Emtiazi

2021

J Basic Microbiol

View full text Add to dashboard Cite

show abstract

“…Microbes display various approaches to overcome the hazardous effects of metals. The metal resistance mechanism are mainly avoidance and tolerance ( Fouda et al., 2018 ; Nedkovska and Atanassov, 1998 ). Fungal tolerance mechanisms involve mobilization, immobilization, and biotransformation.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation and tolerance of zinc ions using Fusarium solani

Sayed

El-Sayed

2020

Heliyon

View full text Add to dashboard Cite

Evaluating the mechanism of tolerance and biotransformation Zn(II) ions by Fusarium solani based on the different physiological was the objective of this work. The physical properties of synthesized ZnONPs was determined by UV-spectroscopy, transmission electron microscope, and X-ray powder diffraction. The structural and anatomical changes of F. solani in response to Zn(II) was examined by TEM and SEM. From the HPLC profile, oxalic acid by F. solani was strongly increased by about 10.5 folds in response to 200 mg/l Zn(II) comparing to control cultures. The highest biosorption potential were reported at pH 4.0 (alkali-treated biomass) and 5.0 (native biomass), at 600 mg/l Zn(II) concentration, incubation temperature 30 °C, and contact time 40 min (alkali-treated biomass) and 6 h (native biomass). From the FT-IR spectroscopy, the main functional groups implemented on this remediation were C–S stretching, C=O C=N, C–H bending, C–N stretching and N–H bending. From the EDX spectra, fungal cellular sulfur and phosphorus compounds were the mainly compartments involved on ZN(II) binding.

show abstract