Metallothionein determination in the liver of the sea bass Dicentrarchus labrax treated with copper and B(a)P

Roméo, Michèle; Cosson, Richard P.; Gnassia‐Barelli, M.; Risso, Christine; Stien, Xavier; Lafaurie, M.

doi:10.1016/s0141-1136(97)00007-x

Cited by 45 publications

(17 citation statements)

References 18 publications

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“…MT induction is known to be modulated by both metals and organic contaminants but previous findings are sometimes contradictory. Simultaneous exposure to Cu and benzo[a]pyrene (B[a]P), was found to greatly increase MT induction in fish liver even when exposure to the individual contaminants reduced MT expression (Roméo et al 1997). On the other hand, MT induction caused by a mixture of metals (Cd, Cu, Pb and Zn) has been found to be suppressed by PAHs (Risso-de Faverney et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

et al. 2009

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Juvenile Solea senegalensis were exposed to fresh sediments from three stations of the Sado estuary (Portugal) in 28-day laboratory assays. Sediments revealed distinct levels of total organic matter, fine fraction, redox potential, trace elements (arsenic, cadmium, chromium, copper, nickel, lead and zinc) and organic contaminants (polycyclic aromatic hydrocarbons, polychlorinated biphenyls and a pesticide: dichloro diphenyl trichloroethane). Organisms were surveyed for contaminant bioaccumulation and induction of two hepatic biochemical biomarkers: metallothionein (MT) and cytochrome P450 (CYP1A), as potential indicators of exposure to metallic and organic contaminants, respectively. Using an integrative approach it was established that, although bioaccumulation is in general accordance with sediment contamination, lethality and biomarker responses are not linearly dependent of the cumulative concentrations of sediment contaminants but rather of their bioavailability and synergistic effects in organisms. It is concluded that metals and organic contaminants modulate both MT and CYP1A induction and it is suggested that reactive oxygen species may be the link between responses and effects of toxicity.

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Section: Discussionmentioning

confidence: 99%

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

et al. 2009

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“…The concentrations used for cadmium and copper were chosen as suggested in some published works and were very much lower than DL50 (Castano et al 1996;Roméo et al 1997). A dose of 500 lg/kg cadmium or copper was sublethal (DL 50 of Cd = 3 mg/kg and DL 50 of Cu = 2.5 mg/kg for Dicentrarchus labrax and Sparus aurata) and usually used in studies of acute toxicity in different fish species in our laboratory (D. labrax and S. aurata).…”

Section: Animal Treatmentmentioning

confidence: 99%

“…MTs act as free radical scavengers and particularly the MT20 isoform (Dondero et al 2005). Benzo-(a)-pyrene at sublethal doses (20 and 18 mg/kg) provokes a repression of MT level by disturbing the structure of hepatocytes of fish (Roméo et al 1997; Van den Hurk et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Metallothionein and metal levels in liver, gills and kidney of Sparus aurata exposed to sublethal doses of cadmium and copper

Ghedira¹,

Jebali²,

Bouraoui³

et al. 2008

Fish Physiol Biochem

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The levels of metallothionein (MT), a biomarker of metal exposure, and of Cd and Cu, known as MT inducers, were investigated in Sparus aurata intraperitoneally injected with 500 microg/kg of Cu and Cd for 2 days. MT and metal concentrations (Cd and Cu) were determined in liver, gills and kidney. MT levels were significantly increased in all investigated tissues, with the highest value in liver of Cu as Cd-treated fishes (3.56-fold and 3.3- fold, respectively). Metal concentrations were statistically different between all tissues. Highest metal concentrations were in the liver. The higher metal concentrations and MT induction levels support the main role of MT in metal homeostasis and detoxification.

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“…On the other hand, the decreased MT level in fish from Sidi Saâd site can be caused by the degeneration of tissue under high pollutant levels (Paris-Palacios et al 2003). Heavy metals such as Cu may provoke a toxic effect on hepatic MT synthesis via oxidative stress generation (Roméo et al 1997). It is not excluded that the exposure to various contaminants determines a decrease of reduced GSH concentration affecting the biosynthesis of hepatic MTs.…”

Section: Discussionmentioning

confidence: 99%

Multimarker approach analysis in common carp Cyprinus carpio sampled from three freshwater sites

Tlili¹,

Jebali²,

Bannı³

et al. 2009

Environ Monit Assess

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The aim of this study is to assess the response of a multimarker approach in common carp Cyprinus carpio sampled from three Tunisian dam lakes selected according to different environmental and ecological characteristics. Glutathione-S-transferase (GST) activity was analyzed in carp liver and used as a phase II detoxification enzyme, hepatic metallothionein content (MTs) was used as a metallic stress indicator, and cholinesterase activities were analyzed in muscle and brain and used as neurotoxicity biomarker. Micronucleus frequency test (MN) as a genotoxicity marker. GST and MT levels showed an increase in fish from the Bir Mcherga site and a decrease in Sidi Saâd site with respect to fish from Nebhana site. Results showed a strong inhibition of cholinesterase activities in fish from Bir Mcherga and Sidi Saâd sites compared to Nebhana site. Relatively high level of MN is reported specially in fish blood from the Bir Mcherga site.

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Metallothionein determination in the liver of the sea bass Dicentrarchus labrax treated with copper and B(a)P

Cited by 45 publications

References 18 publications

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

Metallothionein and metal levels in liver, gills and kidney of Sparus aurata exposed to sublethal doses of cadmium and copper

Multimarker approach analysis in common carp Cyprinus carpio sampled from three freshwater sites

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