Metallothionein, a marker of antiapoptosis, is associated with clinical forms of oral lichen planus

Allon, Irit; Ofir, Merav; Vered, H.; Hirshberg, Abraham

doi:10.1111/jop.12188

Cited by 4 publications

(11 citation statements)

References 29 publications

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“…In the present study, we observed that MT expression is mainly observed in basal and parabasal layers of the oral epithelium, as previously described ( 11 , 19 , 20 ). In 1992, Hanada and collaborators explained that reactivity for MT in cutaneous lesions of lichen planus was concentrated in the granular layer of the epidermis, in the same way of healthy skin, but was less evident in the former ( 10 ).…”

Section: Discussionsupporting

confidence: 90%

“…Regarding to oral lesions, it was only in 2014 that Allon and co-workers described that epithelial MT expression was significantly higher in keratotic OLP than in atrophic and erosive forms ( 11 ). In our work, we also observed that MT expression was less evident in red than white lesions of OLP.…”

Section: Discussionmentioning

confidence: 99%

“…Allon et al described MT expression in the lowest layers of the oral epithelium and in the inflammatory infiltrate of OLP-affected patients ( 11 ). Higher MT expression has been observed in hyperkeratotic (white) rather than in atrophic/erosive (red) lesions of OLP, presumably due to the more pronounced anti-apoptotic response in reticular forms of the disease ( 11 ). In contrast, amalgam, which is one of the most frequent precipitating factors for OLL, can stimulate MT expression ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

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Differential metallothionein expression in oral lichen planus and amalgam-associated oral lichenoid lesions

Mendes

Servato²,

Borges³

et al. 2018

Med Oral

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BackgroundOral lichen planus (OLP) is a chronic inflammatory disease mediated by T cells, which manifests as reticular (white) or erosive (red) lesions, that are eventually painful. Oral lichenoid lesion (OLL) are distinguished from OLP by the presence of precipitating factors. The aim of this study was to evaluate whether the presence of metallothionein, which is involved in anti-apoptotic pathways and the anti-oxidative response, could serve as a differential diagnostic for OLP and OLL.Material and MethodsWe evaluated the expression of metallothionein in 40 cases of OLP and 20 cases of OLL using immunohistochemistry.Results and ConclusionsWhite OLP has higher concentrations of metallothionein than red OLP in basal and parabasal layers. Moreover, metallothionein was more frequently observed in the cytoplasm and nuclei of basal cells in OLP patients compared to the same regions of OLL cases. Metallothionein levels are related to OLP severity and may contribute to a differential diagnosis between OLP and OLL. Key words:Oral lichen planus, oral lichenoid lesions, autoimmune disorders, metallothionein, immunohistochemistry.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential metallothionein expression in oral lichen planus and amalgam-associated oral lichenoid lesions

Mendes

Servato²,

Borges³

et al. 2018

Med Oral

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“…The results of the present study are in line with our previous study that showed a significant increased expression of metallothionein in the keratotic form of OLP. Metallothionein as an anti‐apoptotic marker suggested an extensive anti‐apoptotic response in the keratotic form of the disease (Allon et al , ), and CRT as a PCR marker (therefore postapoptotic marker) was elevated in symptomatic patients.…”

Section: Discussionmentioning

confidence: 99%

“…The antibodies used for immunohistochemistry including positive and negative controls are depicted in Table . The immunomorphometric analysis was carried out as previously described (Allon et al , ). First, the entire slide was screened at ×40 magnification and the region of interest (ROI) was located.…”

Section: Methodsmentioning

confidence: 99%

Programmed cell removal biomarkers calreticulin and CD47 implicated in oral lichen planus

2015

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Estudo imuno-histoquímico da expressão de metalotioneína e proteína p16 em líquen plano e reações liquenóides orais

Mendes¹

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Lichen planus is a chronic inflammatory disease mediated by T cells of unknown cause. Skin, nails and mucous membranes can be affected, and the disease is more common in middleaged adults, especially in women. Oral lichen planus (OLP) manifests as reticular (white) and erosive (red) lesions that are eventually painful. Histologically, it is characterized by dense lymphocytic infiltrate beneath the epithelium, apoptosis of keratinocytes, liquefaction of the basal layer, epithelial hyperplasia and hyperkeratosis. Oral lichenoid reactions (OLR) are distinguished from OLP by the presence of precipitating factors, unilateral lesions and diffuse inflammatory infiltrate. Metallothionein is a protein involved in anti-oxidative response and anti-apoptotic pathways. Ki-67 is a cellular proliferation marker and is expressed only in cells that are in cell division (phases G1, S, G2 and M of the cell cycle). The tumor suppressor gene p16 is involved in the pRb pathway and participates in the regulation of the cell cycle, repairing possible damage to DNA. The aim of this study was to evaluate possible molecular differences, related to oxidative stress and cell proliferation between OLP and OLR, and the two major forms of OLP in order to better understand the pathogenesis of these lesions and facilitate the differential diagnosis between them. Immunohistochemistry for metallothionein, Ki-67 and p16 detection was performed in 42 and 23 cases of OLP and OLR, respectively.Reactivity for metallothionein was more frequently observed in OLP cases than in OLR cases (p = 0,01; Mann-Whitney U test). Significant difference was found between the reticular and atrophic lesions of OLP for p16 protein (p = 0,04; Mann-Whitney U test). There was no significant difference between the lesions in relation to Ki-67 antigen. The results of this study suggest that metallothionein protein may be a useful marker of differential diagnosis between OLP and OLR, and that p16 protein may be a differential marker of reticular and atrophic/erosive lesions of OLP.

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Metallothionein, a marker of antiapoptosis, is associated with clinical forms of oral lichen planus

Abstract: The pattern of expression of MT and caspase-2 in OLP suggests an extensive antiapoptotic response in the keratotic form of the disease. Symptomatic patients may benefit from therapy targeted to apoptosis in the future.

Cited by 4 publications

References 29 publications

Differential metallothionein expression in oral lichen planus and amalgam-associated oral lichenoid lesions

Differential metallothionein expression in oral lichen planus and amalgam-associated oral lichenoid lesions

Programmed cell removal biomarkers calreticulin and CD47 implicated in oral lichen planus

Estudo imuno-histoquímico da expressão de metalotioneína e proteína p16 em líquen plano e reações liquenóides orais

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