METALLOTHIONEIN-2A (RS1610216&RS28366003) GENE POLYMORPHISMS AND THE RISK OF STOMACH ADENOCARCINOMA

Abstract

BACKGROUND: Gastric cancer is the fourth most common cause of worldwide cancer. Also in contrast to the huge advances in curing, the chance of living is very low even in surgery cases. Having a genetic predisposition plays an important role in cancer development. The association between Metallothionein-2A gene polymorphisms and the risk of adenocarcinoma has been widely studied, yet there is only one study on stomach diseases. OBJECTIVE: In this study, we aimed to investigate the association between 2 (MT-2A) polymorphisms and adenocarcinoma. METHODS: This cross-sectional case control study was performed between Mach 2014 and January 2015 at the Tuba Hospital of Sari, Iran. Peripheral blood samples were collected in EDTA tube. DNA extraction was performed using the spin column procedure. The MT-2A polymorphisms MT-2A (rs1610216), (rs28366003) were determined by polymerase chain reaction-restriction fragment length polymorphism analysis in 95 a topic adenocarcinoma patients and 90 healthy individuals from Iranian population. RESULTS: The MT-2A rs1610216 polymorphism increased the risk of adeno carcinoma in our Iranian population [OR: 3.8533; 95%CI, 1.3155-11.2869; P=0.0139] and rs28366003 [OR: 4.0978; 95%CI, 1.2521-13.4108; P=0.0197]. CONCLUSION: The MT-2A gene polymorphism was associated with the risk of adenocarcinoma in the Iranian population.

Quantitative assessment of the association of polymorphisms in the metallothionein 2A gene with cancer risk

“…found that, compared with individuals with the AA genotype at rs28366003, individuals with the GG genotype had a significantly increased risk of gastric cancer, based on 95 patients with gastric cancer and 90 healthy individuals in Iran. 10 In the current pooled analysis, we confirmed that MT2A rs28366003 was associated with the risk of cancer, including breast cancer and prostate cancer. Furthermore, we observed this association of rs28366003 and cancer risk in both Asian and Caucasian subpopulations.…”

“…Sensitivity analysis for rs28366003 showed that no individual study significantly changed the pooled ORs. However, sensitivity analysis for rs1610216 showed a significant change in the pooled ORs after the study of Shokrzadeh et al 10 was removed (Figure 3). Potential publication bias was evaluated by two test methods TSA showed that sample size was insufficient to obtain a significant result; thus, more studies are required ( Figure 5).…”

“…found that compared with the TT genotype, the CC genotype increased the risk of gastric cancer in an Iranian population. 10 Starska et al. found that rs1610216 was not associated with the risk of cancer, including inverted papilloma and squamous cell laryngeal cancer, in a Polish population.…”

“…Moreover, rs28366003 A>G might be associated with the risk of multiple cancers, including stomach adenocarcinoma, breast cancer, inverted papilloma, laryngeal cancer, and prostate cancer. [10][11][12][13][14][15] The rs1610216 T>C SNP might only be associated with the risk of stomach adenocarcinoma. 10 However, previous casecontrol studies had small sample sizes and each focused on only a single type of cancer, and lacked comprehensive assessment of the association of MT2A rs28366003 and rs1610216 SNPs with cancer risk.…”

“…[10][11][12][13][14][15] The rs1610216 T>C SNP might only be associated with the risk of stomach adenocarcinoma. 10 However, previous casecontrol studies had small sample sizes and each focused on only a single type of cancer, and lacked comprehensive assessment of the association of MT2A rs28366003 and rs1610216 SNPs with cancer risk. Hence, in this study, our aim was to quantitatively assessed these associations by meta-analysis as a means to identify genetic biomarkers for predicting cancer risk.…”