Metallothionein 1 (CcMT1) of pigeonpea (Cajanus cajan, L.) confers enhanced tolerance to copper and cadmium in Escherichia coli and Arabidopsis thaliana

Sekhar, Kondapalli Venkata Gowri Chandra; Priyanka, Bhyri; Reddy, V.; Rao, K. V.

doi:10.1016/j.envexpbot.2011.02.017

Cited by 90 publications

(24 citation statements)

References 39 publications

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“…The present study confirmed that transgenic tobacco plants expressing EhMT1 exhibit enhanced Cu tolerance and significantly improved growth of seedlings in the presence of excess Cu. Similar results have been reported previously: overexpression of CcMT1 from Cajanus cajan increased Cu and Cd tolerance in E. coli and Arabidopsis [38]; expression of the seed-specific mt4a gene in Arabidopsis vegetative tissues increased Cu and Zn tolerance [39]; and overexpression of BrMT1 from B. rapa in both the chloroplasts and cytosol resulted in enhanced Cd resistance in transgenic Arabidopsis [24].…”

Section: Discussionsupporting

confidence: 88%

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Xia

Yuan

et al. 2012

Journal of Hazardous Materials

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Section: Discussionsupporting

confidence: 88%

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Xia

Yuan

et al. 2012

Journal of Hazardous Materials

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“…E. coli P4 could resist Cd +2 up to 10.6 mM. Several investigations have showed resistance in E. coli against Cd +2 (Ansari and Malik 2007;Helbig et al 2008;Sekhar et al 2011). Kafilzadeh et al (2013) reported E. coli which could resist Cd +2 up to 3 mM whereas Brocklehurst and Morby (2000) reported resistance against Cd +2 in E. coli up to 1.2 mM.…”

Section: Discussionmentioning

confidence: 96%

Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium

Khan

Nisar

Hussain

et al. 2015

Appl Microbiol Biotechnol

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A cadmium-resistant bacterium was isolated from industrial wastewater and identified as Escherichia coli (dubbed as P4) on the basis of morphological, biochemical tests and 16S rRNA ribotyping. It showed optimum growth at 30 °C and pH 7. E. coli P4 found to resist Cd(+2) (10.6 mM) as well as Zn(+2) (4.4 mM), Pb(+2) (17 mM), Cu(+2) (3.5 mM), Cr(+6) (4.4 mM), As(+2) (10.6 mM), and Hg(+2) (0.53 mM). It could remove 18.8, 37, and 56 % Cd(+2) from aqueous medium after 48, 96, and 144 h, respectively. Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and Energy-dispersive X-ray (EDX) analysis also confirmed the biosorption of Cd(+2) by E. coli P4. However, temperature and pH were found to be the most critical factors in biosorption of Cd(+2) by E. coli P4. Cd(+2) stress altered E. coli P4 cell physiology analyzed by measuring glutathione (GSH) and non-protein thiol (cysteine) levels which were increased up to 130 and 48 %, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) showed alteration in the expression levels of ftsZ, mutS, clpB, ef-tu, and dnaK genes in the presence of Cd(+2). Total protein profiles of E. coli P4 in the absence and presence of Cd(+2) were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), which showed remarkable difference in the banding pattern. czcB gene, a component of czcCBA operon, was amplified from genomic DNA which suggested the chromosomal-borne Cd(+2) resistance in E. coli P4. Furthermore, it harbors smtAB gene which plays a significant role in Cd(+2) resistance.

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“…This is supported by overexpression experiments, e.g., Vicia faba guard cells overexpressing A. thaliana AtMT2a and AtMT3 can tolerate higher levels of Cd than untransformed cells (Lee et al 2004). Similarly, the overexpression of CcMT2 from legume Cajanus cajan in A. thaliana induces both Cd and Cu tolerance and allows both metals to accumulate without affecting the expression of endogenous transporters (Sekhar et al 2011).…”

Section: Metallothioneins and Ferritinsmentioning

confidence: 96%

Plant Responses to Heavy Metal Toxicity

Manara

2012

SpringerBriefs in Molecular Science

188

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Plants, like all other organisms, have evolved different mechanisms to maintain physiological concentrations of essential metal ions and to minimize exposure to non-essential heavy metals. Some mechanisms are ubiquitous because they are also required for general metal homeostasis, and they minimize the damage caused by high concentrations of heavy metals in plants by detoxification, thereby conferring tolerance to heavy metal stress. Other mechanisms target individual metal ions (indeed some plants have more than one mechanism to prevent the accumulation of specific metals) and these processes may involve the exclusion of particular metals from the intracellular environment or the sequestration of toxic ions within compartments to isolate them from sensitive cellular components. As a first line of defense, many plants exposed to toxic concentrations of metal ions attempt to prevent or reduce uptake into root cells by restricting metal ions to the apoplast, binding them to the cell wall or to cellular exudates, or by inhibiting long distance transport. If this fails, metals already in the cell are addressed using a range of storage and detoxification strategies, including metal transport, chelation, trafficking, and sequestration into the vacuole. When these options are exhausted, plants activate oxidative stress defense mechanisms and the synthesis of stress-related proteins and signaling molecules, such as heat shock proteins, hormones, and reactive oxygen species.

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Metallothionein 1 (CcMT1) of pigeonpea (Cajanus cajan, L.) confers enhanced tolerance to copper and cadmium in Escherichia coli and Arabidopsis thaliana

Cited by 90 publications

References 39 publications

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Overexpression of Elsholtzia haichowensis metallothionein 1 (EhMT1) in tobacco plants enhances copper tolerance and accumulation in root cytoplasm and decreases hydrogen peroxide production

Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium

Plant Responses to Heavy Metal Toxicity

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