Metallosphaera javensis sp. nov., a novel species of thermoacidophilic archaea, isolated from a volcanic area

Hofmann, Marika; Norris, Paul R.; Malik, Luise; Schippers, Axel; Schmidt, Gert; Wolf, Jacqueline L.; Neumann‐Schaal, Meina; Hedrich, Sabrina

doi:10.1099/ijsem.0.005536

Cited by 4 publications

(3 citation statements)

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“…The combined DCM phases were dried under a stream of nitrogen. Dried extracts were resuspended in hexane:isopropanol:water (718:271:10 v/v/v) (150 µL/20 mg biomass) and analyzed by HPLC-MS, as described previously [21].…”

Section: Methodsmentioning

confidence: 99%

Thiorhodovibrio frisius and Trv. litoralis spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities

Methner,

Kuzyk,

Petersen

et al. 2023

Microorganisms

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In the pursuit of cultivating anaerobic anoxygenic phototrophs with unusual absorbance spectra, a purple sulfur bacterium was isolated from the shoreline of Baltrum, a North Sea island of Germany. It was designated strain 970, due to a predominant light harvesting complex (LH) absorption maximum at 963–966 nm, which represents the furthest infrared-shift documented for such complexes containing bacteriochlorophyll a. A polyphasic approach to bacterial systematics was performed, comparing genomic, biochemical, and physiological properties. Strain 970 is related to Thiorhodovibrio winogradskyi DSM 6702T by 26.5, 81.9, and 98.0% similarity via dDDH, ANI, and 16S rRNA gene comparisons, respectively. The photosynthetic properties of strain 970 were unlike other Thiorhodovibrio spp., which contained typical LH absorbing characteristics of 800–870 nm, as well as a newly discovered absorption band at 908 nm. Strain 970 also had a different photosynthetic operon composition. Upon genomic comparisons with the original Thiorhodovibrio strains DSM 6702T and strain 06511, the latter was found to be divergent, with 25.3, 79.1, and 97.5% similarity via dDDH, ANI, and 16S rRNA gene homology to Trv. winogradskyi, respectively. Strain 06511 (=DSM 116345T) is thereby described as Thiorhodovibrio litoralis sp. nov., and the unique strain 970 (=DSM 111777T) as Thiorhodovibrio frisius sp. nov.

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Section: Methodsmentioning

confidence: 99%

Thiorhodovibrio frisius and Trv. litoralis spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities

Methner,

Kuzyk,

Petersen

et al. 2023

Microorganisms

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“…Intact polar lipids were extracted from freeze-dried material (approx. 30 mg) using a modified Bligh and Dyer extraction as described previously (28)(29)(30)(31). Briefly, two extractions each were performed using methanol/dichloromethane (DCM)/50 mM phosphate buffer pH 7-8 (2:1:0.8 v/v/v) and methanol/DCM/0.3 M trichloroacetic acid pH 2-3 (2:1:0.8 v/v/v).…”

Section: Lipid and Carbohydrate Profile Analyses By Mass Spectrometrymentioning

confidence: 99%

“…Archaeal lipids were separated on a YMC-Triart Diol column (150 x 2.0 mm, 1.9 µm particles) and analyzed in positive ESI mode by mass spectrometry on an Agilent 6545 Q-ToF mass spectrometer (Agilent, Waldbronn, Germany) as described previously (28,32). Mass spectra were recorded in the mass range of m/z 300-2000.…”

Section: Lipid and Carbohydrate Profile Analyses By Mass Spectrometrymentioning

confidence: 99%

Expanding the cultivable human archaeome:Methanobrevibacter intestinisp. nov. and strainMethanobrevibacter smithii “GRAZ-2”from human feces

Weinberger,

Mohammadzadeh,

Blohs

et al. 2024

Preprint

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Two mesophilic, hydrogenotrophic methanogens, WWM1085 and M. smithii GRAZ-2 were isolated from human fecal samples. WWM1085 was isolated from an individual in the USA, and represents a novel species with in the genus Methanobrevibacter. M. smithii GRAZ-2 (= DSM 116045) was retrieved from fecal samples of a European, healthy female and represents a novel strain within this genus. Both Methanobrevibacter representatives form non-flagellated, short rods with variable morphologies and the capacity to form filaments. Both isolates showed the typical fluorescence of F420 and methane production. Compared to M. smithii GRAZ-2, WWM1085 did not accumulate formate when grown on H2 and CO2. The optimal growth conditions were at 37C, and pH 7. Full genome sequencing revealed a genomic difference of WWM1085 to the type strain of M. smithii PS (type strain; DSM 861), with 93.55% ANI and major differences in the sequence of its mcrA gene (3.3% difference in nucleotide sequence). Differences in the 16S rRNA gene were very minor and thus distinction based on this sequence might not be possible. M. smithii GRAZ-2 was identified as a novel strain within the Methanobrevibacter genus (ANI 99.04 % to M. smithii PS). Due to the major differences of WWM1085 and M. smithii type strain PS in phenotypic, genomic and metabolic features, we propose M. intestini sp. nov. as a novel species with WWM1085 as the type strain (DSM 116060T = CECT 30992).

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Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation – part A

Sand

Schippers

Hedrich

et al. 2022

Appl Microbiol Biotechnol

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Bioleaching of metal sulfides is performed by diverse microorganisms. The dissolution of metal sulfides occurs via two chemical pathways, either the thiosulfate or the polysulfide pathway. These are determined by the metal sulfides’ mineralogy and their acid solubility. The microbial cell enables metal sulfide dissolution via oxidation of iron(II) ions and inorganic sulfur compounds. Thereby, the metal sulfide attacking agents iron(III) ions and protons are generated. Cells are active either in a planktonic state or attached to the mineral surface, forming biofilms. This review, as an update of the previous one (Vera et al., 2013a), summarizes some recent discoveries relevant to bioleaching microorganisms, contributing to a better understanding of their lifestyle. These comprise phylogeny, chemical pathways, surface science, biochemistry of iron and sulfur metabolism, anaerobic metabolism, cell–cell communication, molecular biology, and biofilm lifestyle. Recent advances from genetic engineering applied to bioleaching microorganisms will allow in the future to better understand important aspects of their physiology, as well as to open new possibilities for synthetic biology applications of leaching microbial consortia. Key points • Leaching of metal sulfides is strongly enhanced by microorganisms • Biofilm formation and extracellular polymer production influences bioleaching • Cell interactions in mixed bioleaching cultures are key for process optimization

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Metallosphaera javensis sp. nov., a novel species of thermoacidophilic archaea, isolated from a volcanic area

Cited by 4 publications

References 50 publications

Thiorhodovibrio frisius and Trv. litoralis spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities

Thiorhodovibrio frisius and Trv. litoralis spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities

Expanding the cultivable human archaeome:Methanobrevibacter intestinisp. nov. and strainMethanobrevibacter smithii “GRAZ-2”from human feces

Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation – part A

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